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Correlation Between Cholinesterase Enzyme Levels and Leukocyte Differential Count in Farmers Exposed to Pesticides Rochmad Agung Purnomo; Alya Sabrina Putri Kalista; Ameilisa Eka Nurriani; Septiani Tri Wulandhari; Mastuti Widi Lestari
Jurnal Biologi Tropis Vol. 26 No. 2 (2026): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i2.11467

Abstract

Pesticide exposure in farmers has the potential to cause health problems, one of which indicated by decreased cholinesterase enzyme levels and changes in the leukocyte profile as part of the immune response. This study aimed to determine the correlation between cholinesterase enzyme levels and leukocyte differential count, including eosinophils, lymphocytes, monocytes, and neutrophils, among vegetable farmers exposed to pesticides in Matesih Village, Matesih District. The study used an observational analytical design with a cross-sectional approach. The study population consisted of 29 male vegetable farmers, from which 22 respondents were selected via purposive sampling based on specific inclusion criteria. Cholinesterase enzyme levels were measured using the Cobas Integra 400 Plus photometric method, while leukocyte differential counts were analyzed flow cytometry with tri-angle laser scatter on the Mindray BC-5130 hematology analyzer. Data analysis was performed using Pearson and Spearman correlation tests adjusted to the distribution of the study data. The results showed that most respondents had cholinesterase enzyme levels that were still within the normal range. The correlation analysis indicated no significant correlation between cholinesterase enzyme levels and leukocyte differential count (eosinophils, lymphocytes, monocytes, or neutrophils) (p > 0.05).
Analisis Perlindungan Hukum Terhadap Etika Pengambilan Sampel Urin Mahasiswa untuk Isolasi DNA Dahlan Sitohang; Fitria Diniah Janah Sayekti; Mastuti Widi Lestari
Khatulistiwa: Jurnal Pendidikan dan Sosial Humaniora Vol. 5 No. 2 (2025): Juni : Khatulistiwa: Jurnal Pendidikan dan Sosial Humaniora
Publisher : AMIK Veteran Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/khatulistiwa.v2i4.5839

Abstract

Vulnerability to violations of patient rights as research subjects is the background for the formation of an effort to protect humans as research subjects. The purpose of this study is to analyze how Legal Protection is related to the collection of student urine samples in the DNA Isolation context and what ethics must be adhered to in taking student urine samples for DNA isolation at Stikesnasional. The research method uses a normative legal research type by examining various types of materials originating from several laws and regulations. Research involving humans as subjects, such as urine sampling for DNA isolation, must be carried out with strict ethical principles, including voluntary consent (informed consent), protection of data confidentiality, and respect for the dignity and rights of subjects. Researchers are required to ensure that the procedure does not endanger the physical and psychological well-being of the subject and minimize the risks that may arise. All research involving humans must obtain approval from an ethics committee to ensure that the research protocol meets applicable ethical and legal standards.