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All Journal AQUATIC SCIENCE & MANAGEMENT Jurnal Biologi Tropis
Ariyati H Fadel
Program Magister Ilmu Perairan, Program Pascasarjana, Universitas Sam Ratulangi
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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The effects of stimulant growth hormones on tissue culture of seaweed Kappaphycus alvarezii in vitro Fadel, Ariyati H; Gerung, Grevo S; Suryati, Emma; Rumengan, Inneke F.M
AQUATIC SCIENCE & MANAGEMENT Edisi Khusus 1 (2013): Mei
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jasm.0.0.2013.2282

Abstract

In order to anticipate the qualified and sustainable seed requirement for seaweed culture, it is necessary to conduct tissue culture for vegetative cultivation of isolated leaves, bud, and stemin an artificial medium enriched with nutrient and growth regulator. The purpose of this study is to obtain newly grown plant in a big quantity in relatively short period of time, with physiological and morphological properties similar to the stocks. Culture media used were Grund Medium and PES with an addition of a growth regulator, IAA (Indol acetic acid) and BAP (Benzil amino purin). The buds produced were buds with similar properties as the parent. The longest bud (1,851 mm) was obtained in Grund Medium with IAA treatment, while the length of bud in PES medium was only 0.612 mm. The number of buds was highest (10,6)  in Grund media with IAA+BAP (1:1) treatment, and 6,82 with IAA treatment in PES media. The survival rate of explants was highest in media enriched with 0.5 mg/L IAA (indol acetic acid). The best media for growing seaweed Kappaphycus alvarezii was Grund Medium© Untuk mengantisipasi kebutuhan bibit yang berkualitas dan tersedia  secara kontinyu, diperlukan suatu upaya kultur jaringan untuk perbanyakan tanaman secara vegetatif dengan mengisolasi bagian tanaman seperti daun, mata tunas, serta batang dalam media buatan secara aseptik yang diperkaya dengan nutrien dan zat perangsang tumbuh. Tujuannya untuk mendapatkan tanaman baru dalam jumlah banyak dalam waktu yang relatif singkat, yang mempunyai sifat fisiologi dan morfologis sama dengan tanaman induknya. Media kultur yang digunakan adalah media Grund Medium dan PES dengan penambahan zat perangsang tumbuh yaitu IAA (Indol acetic acid) dan BAP (Benzil amino purin). Tunas yang dihasilkan merupakan anakan yang mempunyai sifat yang sama dengan induknya.  Panjang tunas tertinggi dicapai pada media Grund Medium dengan perlakuan IAA (1,851 mm) dan media PES sebesar 0,612 mm. Sedangkan jumlah tunas tertinggi dicapai perlakuan IAA+BAP (1:1) sebesar 10,6 pada media Grund dan perlakuan IAA sebesar 6,82 pada media PES. Untuk tingkat kelangsungan hidup (sintasan) eksplan yang paling baik pada media yang diberikan pupuk IAA (indol acetic acit) dengan kosentrasi 0,5 mg/L. sedangkan media yang baik untuk pertumbuhan rumput laut Kappaphycus alvarezii adalah media Grund Medium©
Analysis of Availability and Size Distribution of Giant Shrimp (Macrobranchium sp) in River Togafo, Ternate City, North Mollucas Province Abubakar, Yuyun; Sunarti, Sunarti; Salim, Fajria Dewi; Rina, Rina; Abubakar, Salim; Serosero, Rugaya; Fadel, Ariyati H
Jurnal Biologi Tropis Vol. 24 No. 2 (2024): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2.7005

Abstract

Giant prawns (Macrobrachium sp) are one of the freshwater aquaculture commodities that have high economic value (Murtidjo, 1992). The demand for giant prawns in Indonesia has only been met by 40% of all existing demand (Tambunan, 2009. The aim of the research was to analyze the abundance of giant prawns and the size distribution of giant prawns in Togafo Village, West Ternate District, Ternate City. The purposive sampling method was used in this research. Then the sampling points that had been determined using purposive sampling were marked with coordinates with the help of the Global Positioning System (GPS). For the research location, it was divided into 3 observation stations. First, the shrimp body length and weight were measured using calipers and analytical scales. Abundance relative = individual/trip and Distribution of length measurements and distribution of shrimp weight measurements can be grouped using the equation (Effendie, 1979) as follows: K = 1 + 3.3 log n. Note: K = Number of Interval Classes, n = Number of Observation Data, log = Logarithm. Based on the results, the highest abundance of giant prawns (Macrobrachium sp) is at station 3 with a value of 0.575 ind., station 1 is 0.336, the lowest is at station 2 with a value of 0.088 ind. Size Distribution The smallest ranges between 40.17-52.19 mm and the largest ranges between 105.25 – 117.27 mm.
Co-Authors Abubakar, Salim Abubakar, Yuyun Emma Suryati Grevo S Gerung Inneke F. M Rumengan Rina Rina Salim, Fajria Dewi Serosero, Rugaya Sunarti