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All Journal AQUATIC SCIENCE & MANAGEMENT CHEMISTRY PROGRESS BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Aquaculture Journal Journal of Fisheries & Marine
Emma Suryati
Balai Penelitian dan Pengembangan Budidaya Air Payau
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Veterinary

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The effects of stimulant growth hormones on tissue culture of seaweed Kappaphycus alvarezii in vitro Fadel, Ariyati H; Gerung, Grevo S; Suryati, Emma; Rumengan, Inneke F.M
AQUATIC SCIENCE & MANAGEMENT Edisi Khusus 1 (2013): Mei
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jasm.0.0.2013.2282

Abstract

In order to anticipate the qualified and sustainable seed requirement for seaweed culture, it is necessary to conduct tissue culture for vegetative cultivation of isolated leaves, bud, and stemin an artificial medium enriched with nutrient and growth regulator. The purpose of this study is to obtain newly grown plant in a big quantity in relatively short period of time, with physiological and morphological properties similar to the stocks. Culture media used were Grund Medium and PES with an addition of a growth regulator, IAA (Indol acetic acid) and BAP (Benzil amino purin). The buds produced were buds with similar properties as the parent. The longest bud (1,851 mm) was obtained in Grund Medium with IAA treatment, while the length of bud in PES medium was only 0.612 mm. The number of buds was highest (10,6)  in Grund media with IAA+BAP (1:1) treatment, and 6,82 with IAA treatment in PES media. The survival rate of explants was highest in media enriched with 0.5 mg/L IAA (indol acetic acid). The best media for growing seaweed Kappaphycus alvarezii was Grund Medium© Untuk mengantisipasi kebutuhan bibit yang berkualitas dan tersedia  secara kontinyu, diperlukan suatu upaya kultur jaringan untuk perbanyakan tanaman secara vegetatif dengan mengisolasi bagian tanaman seperti daun, mata tunas, serta batang dalam media buatan secara aseptik yang diperkaya dengan nutrien dan zat perangsang tumbuh. Tujuannya untuk mendapatkan tanaman baru dalam jumlah banyak dalam waktu yang relatif singkat, yang mempunyai sifat fisiologi dan morfologis sama dengan tanaman induknya. Media kultur yang digunakan adalah media Grund Medium dan PES dengan penambahan zat perangsang tumbuh yaitu IAA (Indol acetic acid) dan BAP (Benzil amino purin). Tunas yang dihasilkan merupakan anakan yang mempunyai sifat yang sama dengan induknya.  Panjang tunas tertinggi dicapai pada media Grund Medium dengan perlakuan IAA (1,851 mm) dan media PES sebesar 0,612 mm. Sedangkan jumlah tunas tertinggi dicapai perlakuan IAA+BAP (1:1) sebesar 10,6 pada media Grund dan perlakuan IAA sebesar 6,82 pada media PES. Untuk tingkat kelangsungan hidup (sintasan) eksplan yang paling baik pada media yang diberikan pupuk IAA (indol acetic acit) dengan kosentrasi 0,5 mg/L. sedangkan media yang baik untuk pertumbuhan rumput laut Kappaphycus alvarezii adalah media Grund Medium©
BIOSORPSI CAMPURAN LOGAM PB2+ DAN ZN2+ OLEH CHAETOCEROS CALCITRANS Hala, Yusafir; Suryati, Emma; Taba, Paulina
CHEMISTRY PROGRESS Vol 5, No 2 (2012)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.5.2.2012.772

Abstract

Penelitian ini bertujuan untuk menentukan dampak jerapan campuran ion logam Pb(II) dan Zn(II) terhadap pertumbuhan mikroalga Chaetoceros calcitrans dan menentukan efisiensi penjerapan kedua ion logam dalam campuran. Pemaparan ion logam dengan berbagai variasi konsentrasi Pb(II) terhadap Zn(II)dilakukan setelah diperoleh pertumbuhan optimum C. calcitrans, yakni pada hari ke-9. Konsentrasi ion logam Pb(II) dan Zn(II) setelah pemaparan ditentukan dengan spektrofotometer serapan atom. Penambahan ion Pb(II) ke dalam Zn(II)membuat penjerapan ion Zn(II) oleh C. calcitrans turun dibandingkan dengan penjerapan ion tunggal Zn(II). Hal yang sama juga terjadi pada penambahan ion Zn(II)ke dalam ion Pb(II). Hasil penelitian menunjukkan bahwa ion Pb(II) lebih banyak terjerap oleh C. calcitrans dibanding ion Zn(II). Effisiensi penjerapan optimum ion Pb(II) sebesar 64,44% pada perbandingan konsentrasi Pb(II) terhadap Zn(II) 45 : 30 ppm sedangkan penjerapan ion Zn(II) yaitu 56,33% pada perbandingan konsentrasi Zn(II) terhadap Pb(II) 15 : 30 ppm.The main purpose of this research was to determine the adsorption effect of the mixture of Pb(II) and Zn(II) on the growth of Chaetoceros calcitrans and to determine the adsorption efficiency of the ions in the mixture. Exposure of Pb(II) and Zn(II)ions was conducted after the optimum growth of C. calcitrans was obtained, that is at the ninth day with the variation of the concentration ratio of Pb(II) to Zn(II). Concentration of Pb(II) and Zn(II) ions after exposure was determined by atomic absorption spectrophotometer. Addition of Pb(II) ion in solution Zn(II) ion resulted in the decrease of the Zn(II)ion adsorbed by C. calcitrans compared to the adsorption of the single ion of Zn(II). The same result was obtained when Zn(II) ion was added in Pb(II) solution. Results showed that the adsorption of Pb2+ ion by C. calcitrans was higher than that of Zn(II) ion. The maximum adsorption efficiency of Pb(II) ion was 64.44% at the Pb(II):Zn(II) ratio of 45:30, whereas that of Zn(II)ion was 56.33% at the Zn(II):Pb(II) ratio of 15:30.
Assessing various administration strategies for dsRNA vaccine delivery: a concise review of VP15-WSSV research progress in tiger shrimp Penaeus monodon Parenrengi, Andi; Lante, Samuel; Suryati, Emma; Rosmiati, Rosmiati; Sulaeman, Sulaeman; Herlinah, Herlinah; Andriani, Yosie
Jurnal Ilmiah Perikanan dan Kelautan Vol. 17 No. 3 (2025): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v17i3.73580

Abstract

Graphical Abstract     Highlight Research 1. The dsRNA application should correspond to the developmental stages of shrimp2. Immersion method was commonly used for larvae for handling many individuals3. Injection technique effectively delivered dsRNA to cells but was unsuitable for large-scale4. Oral administration of pellet-enriched dsRNA was applicable to apply on shrimp larvae, juveniles, and broodstocks     Abstract RNAi technology offers a novel powerful approach to silence gene expression by introducing double-stranded RNA (dsRNA) into the cell to degrade the mRNA at the post-transcriptional stage. An administration method of dsRNA delivery is one of the main considerations in applying the dsRNA vaccine for controlling pathogen infections. This mini-review was focused on the evaluation of three different methods (immersion, injection, and oral administration) of VP15-dsRNA vaccine delivery to the tiger shrimp post-WSSV challenge test. The immersion method was generally applied for the larval stage of tiger shrimp and seemed to be a simple technique for a large number of individuals in a small tank. The VP15-dsRNA application by immersion improved the survival of tiger shrimp larvae by 3.9% compared to the control groups. The injection technique was an effective way to deliver dsRNA to the cell, but it is difficult to apply in a large number of individuals or populations. The injection of VP15-dsRNA increased significantly the survival rate, proPO, and THC of tiger shrimp. A higher survival rate (75%) was exhibited in tiger shrimp injected with in vivo and in vitro VP15-dsRNA than in the control. Oral administration by pellet-enriched VP15-dsRNA was a useful way for larvae, juveniles, and broodstocks, but it has limitations since the pellet leaches into the water. The application of the VP15-dsRNA vaccine on the feed significantly enhanced the 26.7% higher survival rate compared to the control. The higher survival was also supported by a higher number of THC. The three VP15-dsRNA delivery methods provide potential approaches to increase tiger shrimp resistance to control pathogen infection
ANTI-BIOFOULING ACTIVITY OF SPONGE Callyspongia pseudoreticulata COMPONENTS EXTRACT AGAINST Balanus amphitrite Rosmiati, Rosmiati; Harlina, Harlina; Parenrengi, Andi; Suryati, Emma; Irmawati, Irmawati
Indonesian Aquaculture Journal Vol 19, No 2 (2024): (December, 2024)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.19.2.2024.147-156

Abstract

Biofouling attached to floating net cages and other aquaculture containers is an obstacle in aquaculture. The presence of biofouling can reduce the quality of the environment and interfere with the health of farmed animals, resulting in large losses. This study assessed the anti-biofouling activity of the Callyspongia pseudoreticulata extract component (EMCp) against the Barnacle Balanus amphitrite. For this purpose, C. pseudoreticulata was extracted with 80% methanol using the maceration method at <40°C to obtain EMCp. B. amphitrite was cultured in an aquarium at 25 ppt. The anti-biofouling activity of EMCp was tested against B. amphitrite larvae using asbestos plates. The study used a Complete Randomized Design with three treatments, namely, A) asbestos plate smeared with EMCp + varnish, B) asbestos plate smeared with varnish, and C) asbestos plate not smeared with EMCp and varnish (control). EMCp toxicity test on the larvae used clear bottles, and the adhesion test using 24-well polystyrene plates. Toxicity observations were done after 24 hours, and the number of dead larvae was calculated. The attachment of the larvae was calculated under a microscope at 10× magnification. Identification of groups of chemical compounds using a thin-layer chromatography chromatogram detected under UV lamps and spraying of reagents. The study found that EMCp effectively inhibited the attachment and growth of B. amphitrites with the LC50 of 150 mg/L. Studies of chemical constituents identified various compounds in the extract, including alkaloids, flavonoids, and terpenoids. These results suggest that C. pseudoreticulata has potential as a natural alternative to chemical-based antifouling agents.
Binary Vector Construction and Agrobacterium tumefaciens-mediated Transformation of Lysozyme Gene in Seaweed Kappaphycus alvarezii handayani, Tri; Alimuddin, Alimuddin; Widyastuti, Utut; Suryati, Emma; Parenrengi, Andi
BIOTROPIA Vol. 21 No. 2 (2014): BIOTROPIA Vol. 21 No. 2 December 2014
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2014.21.2.348

Abstract

Ice-ice disease is the biggest problem in the cultivation of seaweed Kappaphycus alvarezii. The disease is caused by bacterial infection and induced by drastic changes of water quality. Lysozyme has the ability to break down bacterial cell wall. The purpose of this research was to construct of a binary vector pMSH1-Lys carrying chicken Lysozyme (Lis) gene and introduce pMSH1-Lis on K. alvarezii. The binary vector expression was transformed into Agrobacterium tumefaciens LBA4404 by triparental mating. Thallus was inoculated with A. tumefaciens carrying pMSH1-Lis and then the transformed thallus was selected by adding 20 mg/L hygromycin to the culture medium. PCR analysis showed that the construction of the binary plasmid pMSH1-Lys was established. Percentage of transformation of pMSH1-Lis on K. alvarezii was 23.56%, while the efficiency of regeneration was 11.32%. PCR analysis showed that three of the regenerated thallus contained Lysozyme gene. Thus, transgenic K. alvarezii was produced successfully and this can be useful for studying the mechanisms of seaweed defense against bacterial infection.
Co-Authors Andi Parenrengi Ariyati H Fadel Grevo S Gerung Harlina Harlina Herlinah Herlinah Inneke F. M Rumengan Irmawati Irmawati Paulina Taba Rosmiati Rosmiati Samuel Lante, Samuel Sulaeman Sulaeman Tri Handayani UTUT WIDYASTUTI Yosie Andriani Yusafir Hala