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Teuku Fadrial Karmil
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Journal : Jurnal Veteriner

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Penggunaan Protein Membran Stadium Bradizoit Toxoplasma gondii untuk Mendiagnosis Toksoplasmosis dengan Metode Intradermal Test Muhammad Hanafiah; Wisnu Nurcahyo; Dwinna Aliza; Teuku Fadrial Karmil
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A research was conducted to find out an alternative diagnose in detecting toxoplasmosis in livestock/animal using intradermal test  from protein membrane of  T. gondii bradizoite stage. Local isolate ofmembrane T. gondii bradizoite stage  was used in the research. Ten of domestic sheep with the age of ± 1year and 10 mice strain Balb/c with the age ± 2 month were used in this research. The reaction ofhypersensitivity on the skin post protein membrane bradyzoite injection was indicated by the process ofskin thickening. The diameter skin thickening was measured using cutimeter, in which diameter e” 10 mmindicated positive diagnose. The result showed that optimal dosage of membran protein bradyzoite thatcould be applied to detect toxoplasmosis in livestock and animal using intradermal test were 0,6 ml and0,2 ml for sheep and mice respectively. The sensitivity and specificity level of antigen use (protein membrane)of T. gondii bradizoite stage from local isolate to diagnose toxoplasmosis in mice using intradermal testwere: 85.0 %  and 66.6 % respectively, while in sheep the sensitivity and specificity level were 85.0 % and66.6 % respectively. It can be concluded that  intradermal  test was appropriate to be implemented fordetecting toxoplasmosis in sheep and mice induced with tachyzoite T. gondii.
AKTIVITAS HIALURONIDASE BAKTERI STREPTOKOKUS GRUP B PADA SUBSTRAT ASAM HIALURONAT THE HYALURONIDASE ACTIVITY OF GROUP B STREPTOCOCCUS IN HYALURONIC ACID SUBSTRATE Zinatul Hayati; Wendy Setiyadi Putranto; Teuku Fadrial Karmil; I Wayan Teguh Wibawan; Sri Budiarti Poerwanto
Jurnal Veteriner Vol 8 No 2 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Streptococcus agalactiae atau yang juga dikenal dengan Streptokokus grup B (SGB) telah diketahui sebagai agen penyebab pneumonia, septikemia dan meningitis neonatal pada manusia dan hewan. Hialuronidase merupakan produk ekstraseluler dari SGB yang menentukan virulensinya. Penelitian ini dilakukan untuk menguji akitvitas hialuronidase dari 10 isolat SGB yang diisolasi dari kasus komplikase obstetri, serta memurnikan dan mengkarakterisasi hialuronidase yang diisolasi dari SGB SV-14 pada substrat asam hialuronat.
Patogenisitas Streptokokus Grup B pada Mencit Neonatus Zinatul HayatiO; Teuku Fadrial Karmil
Jurnal Veteriner Vol 10 No 4 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Group B Streptococci (GBS) are the major cause of serious infections in neonates, including pneumonia,septicemia and meningitis. Considering that GBS bacteria founded in Indonesia in our previous study isdissimilar as what have been found in foreign country particularly in its serotype distribution, thereforethis research is extremely essential to be conducted in Indonesia in order to searching the vaccine candidateto be used in Indonesia. This research focused in pathogenicity test of each GBS isolates on animal model.Pathogenicity experiment has been done by early onset septicemia model approach to neonatal mouse.Pathogenicity test result that SR-7 isolates can cause the death of neonatal mouse 100% in early-onsetinfection. Whereas, SV-2 isolates cannot cause the neonatal mouse neither in early-onset nor in late-onset.From the serotype determination result at previous research found that SR-7 isolates is serotype isolatesVI whilst SV-2 isolates is serotype III. This result has shown that SR-7 (serotype VI) isolates is the mostpathogen and the SV-2 isolates is less pathogen.
Co-Authors Dwinna Aliza I wayan Teguh Wibawan Muhammad Hanafiah Sri Budiarti Poerwanto Wendy Setiyadi Putranto Wisnu Nurcahyo Zinatul Hayati Zinatul HayatiO