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The Effect of Stage of Estrous Cycle on The Development of Bovine Embryo Matured and Fertilized in Vitro I. Djuwita; B. Purwantara; Y. Sukra; M. Fahrudin; A. Winarto
Media Veteriner Vol. 3 No. 1 (1996): Media Veteriner
Publisher : Media Veteriner

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The objective ofthis study is to examine the effect of stage of estrous cycle on in vitro development of bovine embryo matured and fertilized in vitro. Cow ovaries were collected from slaughter house and were kept in a physiological solution. Oocytes were aspirated using GI8 neddle connected to 10 mL syringe containing phosphate buffer saline (PBS). After being washed, oocytes were matured in vitro in Tissue Culture Medium (TCM) 199 a physiological in 5% CO2 incubator at 39°C for 20-22 hours. In vitro fertilization was done in BO (Brackett and Oliphant) solution for 8 hrs, using frozen semen. Embryos were further cultured in either TCM-199 supplemented with 5% cow superovulated serum or chemically defined-serum free medium. If ovaries were classified due to their estrous stages, i.e. luteal and follicular, both cleavage (fertilization) and development rates did not show any differences (p < 0,5) in both treatment. The cleavage rate of oocytes collected from the follicular and luteal stages were 75.0% and 75.6%. respectively even though was lower if compared to that withaout classification (83.5 %). The development rate were 20,0% and 23,0%, higher, compare to that without classification, i.e. 13.0%. Nevertheless, if chemically defined-serum free medium was used, the two treatments showed differences (p < 0,5) in both cleavage and development rates. The ratio between luteal and follicular stages were 68,0% : 80,0% and 53,6%: 65,7% for cleavage and development rates, respectively.

Pemberian Literasi Digital Marketing dan Pendampingan Penjualan Produk UMKM di Lampung melalui Aplikasi Platform Digital, Periklanan dan Konten Marketing Mahrinasari MS; Remy Yudha Saputra; Ayu Gita Permata; Apridalia Apridalia; Haysi Muliyana; Lusia Dewi Sulistiati; M. Fahrudin
J-ABDIPAMAS (Jurnal Pengabdian Kepada Masyarakat) Vol 8, No 1 (2024): April 2024
Publisher : IKIP PGRI Bojonegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30734/j-abdipamas.v8i1.4018

ABSTRACTThe Community Service Activities carried out are Providing Digital Marketing Literacy and Product Sales Assistance to MSMEs through the Digital Platform Application, Advertising, and Marketing Content with the aim of providing the community, especially MSME business actors, with literacy related to digital marketing and can increase sales of their products using the Digital Platform so that they can open up opportunities to increase customer potential with "suspect" customer level and increase the opportunity for many customers to appear at the "active” customer level because of the ease of ordering on the digital platform used. The Digital Marketing literacy provided can be the basis for MSME players to always innovate in marketing their products.Keywords: MSMEs, Digital Marketing, Digital Platforms, Advertising, Content Marketing ABSTRAKKegiatan Pengabdian Masyarakat yang dilakukan adalah Pemberian Literasi Digital Marketing dan Pendampingan Penjualan Produk pada UMKM melalui Aplikasi Platform Digital, Periklanan dan Konten Marketing bertujuan agar masyarakat khususnya pelaku usaha UMKM mendapatkan literasi terkait digital marketing dan dapat meningkatkan penjualan produknya menggunakan Platform Digital sehingga dapat membuka peluang untuk menambah potensi pelanggan dengan level “suspect” dan memperbesar peluang munculnya banyak pelanggan dengan level “active customer” karena kemudahan pemesanan pada platform digital yang digunakan. Literasi Digital Marketing yang diberikan dapat menjadi dasar pelaku UMKM untuk selalu berinovasi dalam pemasaran produknya.Kata Kunci: UMKM, Digital Marketing, Platform Digital, Periklanan, Konten Marketing

The potency of sericin as an alternative protein in collection and maturation media to support in vitro bovine oocyte maturation Karja, N. W. K.; Satrio, F. A.; Setiyono, A.; Prasetyaningtyas, W. E.; Fahrudin, M.
Journal of the Indonesian Tropical Animal Agriculture Vol 49, No 3 (2024): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.49.3.224-230

The purpose of this study was to evaluate the potency of sericin as a substitute for BSA (bovine serum albumin) to support the in vitro maturation of bovine oocytes. Cumulus-oocytes complexes were collected with BSA or 0,1% sericin, and then matured either in media with BSA or sericin. The maturation rate was evaluated based on the meiotic status of oocytes. The incidence of DNA fragmentation in oocytes was assessed by TUNEL. The percentage of oocytes reaching the MII stage in the media either with BSA or sericin was significantly higher than those of oocytes collected and matured in BSA only. Although the maturation rate of oocytes collected with BSA and then matured with sericin was comparable to oocytes in BSA-BSA groups (P>0.05) the rate was similar to oocytes collected with sericin and then even matured with BSA or sericin (P>0.05). There was no significant difference in the incidence of DNA fragmentation among the treatment groups (P>0.05), the indices of DNAfragmented oocytes were found around 17-20 %. In conclusion, Sericin has the potency to replace BSA as a source of protein either in collection media and/or in maturation media as well as potentially preventing the incidence of DNA fragmentation in oocytes.

Pemberian Literasi Digital Marketing dan Pendampingan Penjualan Produk UMKM di Lampung melalui Aplikasi Platform Digital, Periklanan dan Konten Marketing MS, Mahrinasari; Saputra, Remy Yudha; Permata, Ayu Gita; Apridalia, Apridalia; Muliyana, Haysi; Sulistiati, Lusia Dewi; Fahrudin, M.
J-ABDIPAMAS (Jurnal Pengabdian Kepada Masyarakat) Vol. 8 No. 1 (2024): April 2024
Publisher : IKIP PGRI Bojonegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30734/j-abdipamas.v8i1.4018

ABSTRACTThe Community Service Activities carried out are Providing Digital Marketing Literacy and Product Sales Assistance to MSMEs through the Digital Platform Application, Advertising, and Marketing Content with the aim of providing the community, especially MSME business actors, with literacy related to digital marketing and can increase sales of their products using the Digital Platform so that they can open up opportunities to increase customer potential with "suspect" customer level and increase the opportunity for many customers to appear at the "active” customer level because of the ease of ordering on the digital platform used. The Digital Marketing literacy provided can be the basis for MSME players to always innovate in marketing their products.Keywords: MSMEs, Digital Marketing, Digital Platforms, Advertising, Content Marketing ABSTRAKKegiatan Pengabdian Masyarakat yang dilakukan adalah Pemberian Literasi Digital Marketing dan Pendampingan Penjualan Produk pada UMKM melalui Aplikasi Platform Digital, Periklanan dan Konten Marketing bertujuan agar masyarakat khususnya pelaku usaha UMKM mendapatkan literasi terkait digital marketing dan dapat meningkatkan penjualan produknya menggunakan Platform Digital sehingga dapat membuka peluang untuk menambah potensi pelanggan dengan level “suspect” dan memperbesar peluang munculnya banyak pelanggan dengan level “active customer” karena kemudahan pemesanan pada platform digital yang digunakan. Literasi Digital Marketing yang diberikan dapat menjadi dasar pelaku UMKM untuk selalu berinovasi dalam pemasaran produknya.Kata Kunci: UMKM, Digital Marketing, Platform Digital, Periklanan, Konten Marketing

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