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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Buletin Penelitian Tanaman Rempah dan Obat BERITA BIOLOGI Buletin Penelitian Tanaman Rempah dan Obat Manilkara: Journal of Bioscience
Fajarudin Ahmad
Lembaga Ilmu Pengetahuan Indonesia (LIPI), Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Immunology & microbiology Medicine & Pharmacology Neuroscience Veterinary

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Journal : BERITA BIOLOGI

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ANALISIS KERAGAMAN GENETIK Musa balbisiana Colla BERDASARKAN MARKA RAPD DAN ISSR Yuyu Suryasari Poerba; Fajarudin Ahmad
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i2.540

Abstract

Wild Musa balbisiana Colla is one of the progenitors of cultivated bananas and plantains. It is originated in Asia, and distributed from India to Papua New Guinea. This study was conducted to assess the molecular diversity of 25 accessions of M. balbisiana based on Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) analyses. RAPD and ISSR fingerprints of these banana varieties were detected by amplifications of nine primers of RAPDs and six primers of ISSRs. RAPD primers produced 84 amplified fragments varying from 150 bp to 2300 bp in size. 21.43 % of the amplification bands were polymorphic. ISSR primers produced 61 amplified fragments varying from 250 bp to 2200 bp in size. 29.30 % of the amplification bands were polymorphic. Based on these results, the 25 accessions of Indonesian M. balbisiana showed a low genetic variation, with coefficient of similarity ranged from 0.81 to 0.99.
Co-Authors Albertus H. Wawo Diyah Martanti Herlina L. Agus Sukamto Tri Handayani Yuyu Suryasari Poerba Yuyu Suryasari Poerba Yuyu Suryasari Poerba