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All Journal Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) INTEGRITAS : Jurnal Pengabdian Community Development Journal: Jurnal Pengabdian Masyarakat Nursing and Health Sciences Journal (NHSJ) Journal of Ocean, Mechanical and Aerospace -science and engineering- (JOMAse) Jurnal Informatika Progres Riset Informasi Kesehatan JPLED
Hidayatul Fajri
Program Studi Agroteknologi, Fakultas Pertanian, Universitas Syiah Kuala
Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Engineering Health Professions Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : Riset Informasi Kesehatan

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The effectiveness of sickle cell anemia treatment using CRISPR Fahira, Adinda Putri Awalia; Widowati, Retno; Fajri, Hidayatul
Riset Informasi Kesehatan Vol 14 No 1 (2025): Riset Informasi Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Harapan Ibu Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30644/rik.v14i1.879

Abstract

The rapid advancement of medical biotechnology plays a crucial role in healthcare practices, particularly in genetic engineering for sickle cell anemia. Currently, there is no definitive cure for sickle cell anemia, but in recent years, gene therapy has been extensively researched as an alternative solution. Gene therapy trials on sickle cell anemia patients have been conducted using CRISPR technology, and its effectiveness is still under investigation. The principle involves manipulating the abnormal hemoglobin gene in erythrocytes, which causes sickle cell disease, by extracting stem cells from the patient’s bone marrow and replacing the faulty gene with a normal one using CRISPR (Clustered Regulatory Interspaced Short Palindromic Repeats). The critical components required are Cas9, an endonuclease restriction enzyme, and gRNA, which guides Cas9 to the target gene for manipulation. Once the target gene is successfully cut by Cas9, DNA polymerase naturally initiates a gene repair mechanism through the HDR (Homologue-directed repair) pathway, a process of repairing damaged DNA sequences in CRISPR. Based on tests conducted on 44 patients, 28 patients showed promising results, with their bone marrow producing functional, normal erythrocytes.
Co-Authors , Gunawarman Akmal, Artha Dini Anggraini, Ezi Baharuddin, Suardi Hi Fahira, Adinda Putri Awalia Fifi Yasmi Hanoselina, Yulia Iip Permana jimi ronald, jimi Jon Affi, Jon Nila Wahyuni Nurjanah, Isnaeni Putra, Rezky Syahemi Retno Widowati Sarumpaet, Calvin Bonar Yuliarti Yuliarti