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All Journal International Journal of Public Health Science (IJPHS) Indonesian Journal of Applied Sciences At-Taqaddum Meditory : The Journal of Medical Laboratory AL-HAYAT: Journal of Biology and Applied Biology Cendekia Journal of Pharmacy JURNAL ANALIS LABORATORIUM MEDIK Indonesian Journal of Global Health research Journal of Nursing and Health (JNH) Akper Yakpermas Banyumas MAHESA : Malahayati Health Student Journal Indonesian Journal of Global Health Research
Fitria Diniah Janah Sayekti
Sekolah Tinggi Ilmu Kesehatan Nasional
Religion Biochemistry, Genetics & Molecular Biology Dentistry Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health Social Sciences

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Hepatotoxicity effect of alcoholic beverages on histology and IL-6 gene detection in Rattus norvegicus using polymerase chain reaction Fitria Diniah Janah Sayekti; Vector Stephen Dewangga; Muhammad Taufiq Qurrohman
International Journal of Public Health Science (IJPHS) Vol 15, No 1: March 2026
Publisher : Intelektual Pustaka Media Utama

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijphs.v15i1.27001

Abstract

The liver is an organ that has an important role in metabolic and detoxification processes. Alcohol consumption will cause metabolic disorders in the liver and can cause changes in histological structures. Interleukin-6 has a profound role in liver pathology and plays an important role in the body's defense mechanism, and contributes to the manifestation of tissue damage. This study was an experimental study using 20 Rattus norvegicus treated with 25% alcohol, and a normal treatment only given distilled water. Histological observations were carried out on each preparation in 5 fields of view. The hepatotoxicity effect of alcohol was observed based on the presence of cell degeneration and necrosis in histological preparations stained with hematoxylin eosin. IL-6 detection with polymerase chain reaction was analyzed qualitatively using electrophoresis. Based on the statistical test results, the sig. (2 tailed) of 0.00 < 0.05 so that it can be seen that there is a significant difference in histological results between the normal control group without treatment and the group induced by alcohol. The administration of alcoholic beverages with 25% ethanol content in vivo for 21 days causes necrosis and bleeding around the hepatocyte cells. IL-6 overview appeared to be stronger in the group with alcohol-induced drinking, it could indicate that giving alcohol causes a higher inflammatory response. This study provides scientific evidence that alcohol consumption significantly damages the liver. Further research can be conducted to increase the sample size and quantitatively assess IL-6 expression.
Detection of adeB and basD Genes in Lung Tissue Samples of Rattus Norvegicus Injected with Acinetobacter Baumannii Augy Sausan Jannati Damartiya; Desi Nur Hasanah; Fitria Diniah Janah Sayekti
Indonesian Journal of Global Health Research Vol. 8 No. 2 (2026): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v8i2.752

Abstract

Acinetobacter baumannii is an opportunistic pathogen and a major cause of nosocomial infections, characterized by high resistance to multiple antibiotics. The success of its colonization and virulence is influenced by resistance factors such as the adeB gene and nutrient acquisition factors such as the basD gene. This study aimed to detect the presence of adeB and basD genes in lung tissue of Rattus norvegicus intravenously injected with clinical isolates of Acinetobacter baumannii. Six healthy female rats were divided into treatment groups with observation periods of 10 hours and 18 hours post-infection. Lung tissues were collected for DNA extraction, followed by PCR amplification using specific primers for adeB (549 bp) and basD (533 bp). Gel electrophoresis results showed that both genes were clearly detected in the bacterial isolate positive control, but not in lung tissue samples post-infection. The failure of detection in tissue samples is likely influenced by the low bacterial load, host-dependent regulation of gene expression, and the presence of PCR inhibitors derived from lung tissue. These findings indicate that although adeB and basD play crucial roles in resistance and virulence, their presence in host tissues is not always detectable by conventional PCR. Therefore, more sensitive detection methods are required for molecular analysis in in vivo models.
Detection of NFKB1 Gene in Patients with Type 2 Diabetes Mellitus and Non Diabetics Using Polymerase Chain Reaction Method Priska Putri Cahyaningtyas; Fitria Diniah Janah Sayekti
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 2 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i2.20856

Abstract

A metabolic condition called type 2 diabetes mellitus is characterized by elevated blood sugar levels because of reduced insulin release by β pancreatic cells. The primary form of NF-kB is Nuclear Factor Kappa Beta subunit-1 (NFKB1), which is a gene that encodes the DNA binding protein (p50). The NFKB1 gene contributes to the oxidative stress and mild inflammatory processes that might exacerbate diabetes. The enzymatic procedure known as Polymerase Chain Reaction (PCR), multiplies a nucleotide sequence in order to identify if type 2 diabetes mellitus or non-diabetic has the NFKB1 gene. The purpose of this study was to detect the presence of the NFKB1 gene in type 2 diabetes mellitus and non-diabetes. The study used a descriptive research method using a purposive sampling technique conducted at the molecular biology laboratory of the Sekolah Tinggi Ilmu Kesehatan Nasional. The respondents in this study were 9 patients with type 2 diabetes mellitus who were prolanes of Puskesmas Wonosari I Klaten and 9 non-diabetic patients in PKK Kadilangu RT 2/RW 1, Baki, Sukoharjo. From the results of the study, it can be seen that the NFKB1 gene was detected after electrophoresis and visualized at 176 bp (base pair). The qualitative presence of the NFKB1 gene in DNA is still detectable, but the level of gene expression in the soil of transcription and translation is not yet known.
The Protective Effect Of Rambutan Honey On Macroscopic And Microscopic Examination Of Pancreas Rattus norvegicus Induced By Traditional Alcoholic Beverages Ciu Fitria Diniah Janah Sayekti; Pramesti Kirana Putri
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 2 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i2.26971

Abstract

The traditional ciu alcoholic beverages is a drink made from distilled sap and sticky tape. Ciu is a type of drink that has an alcohol content of 25%. Alcoholic beverages will be toxic if consumed in excess. The pancreas is one of the organs that undergoes a process of damage when toxicmaterials or metabolites accumulate in the organ. Rambutan honey is a natural ingredient that contains flavonoid compounds. Flavonoids act as antioxidants which can reduce damage to pancreatic cells due to toxic substances. The aim of this study was to determine the protective effect of rambutan honey on the macroscopic and microscopic examination of white rats (Rattus norvegicus) induced by traditional ciu alcohol. This research is experimental with 5 treatment groups including 1 control normal, 1 control negative and 4 ciu and honey treatments. Pancreatic tissue was observed macroscopically for color and texture. Microscopic observations were observed in the form of degeneration and necrosis. The microscopic results showed that there was a color difference between the control group and the alcohol-induced honey treatment group. The results of microscopic observations were known to show degeneration and necrosis in the positive group and the treatment group. The results of the study based on the ANOVA test obtained <0.05. Based on the Anova test and Duncan test it can be concluded that rambutan honey has a protective effect on the pancreatic histopathology of white rats (Rattus norvegicus) in the form of normal cells and degenerated cells in groups K2 and K3
Hubungan Viral Load HIV1-RNA terhadap Jumlah Trombosit Pada Pasien yang Terdeteksi HIV di Surabaya Ardhiyani Ardhiyani; Fitria Diniah Janah Sayekti
MAHESA : Malahayati Health Student Journal Vol 6, No 3 (2026): Volume 6 Nomor 3 (2026)
Publisher : Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/mahesa.v6i3.21004

Abstract

ABSTRACT HIV (Human Immunodeficiency Virus) is a virus that attacks the immune system and can lead to AIDS. Thrombocytopenia, which is a reduction in platelet count, is frequently found in HIV patients and may impact their prognosis. This study aims to analyze the relationship between platelet count and HIV-1 RNA viral load in HIV patients detected at Prodia Surabaya. This study aims to determine the relationship between platelet count and HIV-1 RNA viral load in HIV patients detected at Prodia Surabaya Clinical Laboratory. This study uses an analytical observational design with a cross-sectional approach. The sample consists of 31 HIV patients who underwent HIV-1 RNA testing at Prodia Surabaya Laboratory from December 2024 to February 2025. Data on HIV-1 RNA viral load and platelet count were analyzed using Spearman’s statistical test. The results show that the majority of patients had an HIV-1 RNA viral load of 4.0 x 10¹ (51.61%) and normal platelet counts (83.88%). Normality tests indicated that both variables (viral load and platelet count) were not normally distributed (p 0.001). Spearman’s test yielded a correlation coefficient of -0.157 with a significance value of p = 0.400, indicating that there is no significant relationship between platelet count and HIV-1 RNA viral load. There is no significant relationship between platelet count and HIV-1 RNA viral load in HIV patients detected at Prodia Surabaya. Other factors such as nutritional status, antiretroviral therapy, and co-morbid infections may contribute to influencing platelet count. Keywords: HIV, Platelet, Thrombocytopenia, Viral Load.  ABSTRAK HIV (Human Immunodeficiency Virus) adalah virus yang menyerang sistem kekebalan tubuh dan dapat menyebabkan AIDS. Trombositopenia, yaitu penurunan jumlah trombosit dalam darah, sering kali ditemukan pada pasien HIV dan dapat mempengaruhi prognosis mereka. Penelitian ini bertujuan untuk menganalisis hubungan antara kadar trombosit dengan viral load HIV-1 RNA pada pasien HIV yang terdeteksi di Prodia Surabaya. Penelitian ini bertujuan untuk mengetahui hubungan antara kadar trombosit dengan viral load HIV-1 RNA pada pasien yang terdeteksi HIV di Laboratorium Klinik Prodia Surabaya. Penelitian ini menggunakan desain analitik observasional dengan pendekatan cross-sectional. Sampel penelitian terdiri dari 31 pasien HIV yang menjalani pemeriksaan HIV-1 RNA di Laboratorium Prodia Surabaya pada periode Desember 2024 hingga Februari 2025. Data viral load HIV-1 RNA dan kadar trombosit dianalisis dengan uji statistik Spearman. Hasil penelitian menunjukkan bahwa mayoritas pasien memiliki viral load HIV-1 RNA 4,0 x 10¹ (51,61%) dan kadar trombosit dalam batas normal (83,88%). Uji normalitas menunjukkan bahwa kedua variabel (viral load dan trombosit) tidak terdistribusi normal (p 0,001). Uji Spearman menghasilkan koefisien korelasi -0,157 dengan nilai signifikansi p = 0,400, yang mengindikasikan bahwa tidak terdapat hubungan yang signifikan antara kadar trombosit dan viral load HIV-1 RNA. Tidak terdapat hubungan yang signifikan antara kadar trombosit dan viral load HIV-1 RNA pada pasien yang terdeteksi HIV di Prodia Surabaya. Faktor-faktor lain seperti status gizi, terapi antiretroviral, dan infeksi ko-morbid mungkin berperan dalam mempengaruhi kadar trombosit. Kata Kunci: HIV, Trombosit, Trombositopenia, Viral Load.
Co-Authors Alfian Marfianto Annisa Banowati Ardhiyani Ardhiyani Augy Sausan Jannati Damartiya Chris Srikandini Damar Ajeng Priyandari Desi Nur Hasanah Dwi Haryami Dwi Haryatmi Exyananda Clorinda Arabella Indah Rismarwati Muhammad Taufiq Qurrahman Muhammad Taufiq Qurrohman Muhammad Taufiq Qurrohman Pramesti Kirana Putri Priska Putri Cahyaningtyas Vector Stephen Dewangga