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All Journal JURNAL PEMULIAAN TANAMAN HUTAN
Yuliah Yuliah
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Pemuliaan Tanaman Hutan Yogyakarta
Agriculture, Biological Sciences & Forestry

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PENGARUH POHON INDUK DAN BAHAN STEK TERHADAP PERTUMBUHAN STEK CABANG TIMOHO (Kleinhovia hospita L) Lukman Hakim; Yuliah Yuliah; Hamdan Adma Adinugraha
Jurnal Pemuliaan Tanaman Hutan Vol 13, No 2 (2019): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (820.089 KB) | DOI: 10.20886/jpth.2019.13.2.123-130

Abstract

Timoho (Kleinhovia hospita L) is a rare plant that has high economic and cultural value for the Javanese people, especially in Yogyakarta. Timoho wood has a beautiful pattern called pelet and is used as a raw material for keris sheath. In Javanese culture, the keris is a masterpiece culture that is used as a weapon, spiritual object and complementary traditional events such as marriage. In Yogyakarta, Timoho trees are difficult to find, currently, so that plant propagation in a generative way also has difficulty due to scarcity of seeds. Therefore, it is necessary to conduct Timoho propagation research through vegetative methods. The purpose of this study was to determine the relationship between the origin of stem cuttings from three Timoho mother trees and the stem section (base, middle, and end) to the success of Timoho stem cuttings. Stem cuttings come from three Timoho trees in the Arboretum of the Center for Forest Research and Development of Forest Biotechnology and Tree Improvement Yogyakarta. The research method used a nested experimental design using 3 mother trees and 3 stem parts (base, middle and end) which were nested in the mother tree. The characters observed were percentage of shoots, number of shoots, length of shoots, and number of leaves. The results showed the percentage of live cuttings varied from 10.9-60.0%, number of shoots 1-7 pieces, number of leaves 3-26 strands and length of shoots 2.00-17.7 cm. The mother tree treatment has a significant effect on the percentage of sprouts and shoot length, while the cuttings material has a significant effect on the length of shoots at the cuttings measurements of the age of 2 months since planted in polybags.
KERAGAMAN GENETIK KAYU KUKU (Pericopsis mooniana Thw) PROVENANS SULAWESI TENGGARA PADA TINGKAT SEMAI Tri Pamungkas Yudohartono; Ari Fiani; Yuliah Yuliah
Jurnal Pemuliaan Tanaman Hutan Vol 13, No 1 (2019): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (334.629 KB) | DOI: 10.20886/jpth.2019.13.1.11-17

Abstract

High demand of kayu kuku (Pericopsis mooniana Thw) has threatened the existence of its populations in natural distribution. Therefore, ex-situ conservation need to be carried out to protect them from extinction. Preparation genetic materials in nursery stage is very important to support the success of establishing ex-situ conservation plot. The objective of this research is to observe the role of genetic factor in the growth characteristics of kayu kuku seedlings in which the seed was originally collected from South East Sulawesi provenance. This research was arranged in Completely Randomized Complete Bock Design with 40 families in three replications. The result showed that genetic difference among the tested families affected the growth characteristics of seedling for height, diameter and sturdiness index. Genotype and phenotype correlation between height and diameter traits were 0.95 and 0.74 respectively. It revealed that high genetic diversity of kayu kuku could be observed in seedling stage, and indicated an important of genetic materials collected from Southeast Sulawesi provenance for further conservation and tree improvement program.
KERAGAMAN GENETIK Aquilaria malaccensis DARI BANGKA BARAT PROVINSI KEPULAUAN BANGKA BELITUNG DAN IMPLIKASINYA UNTUK PENGELOLAAN TEGAKAN BENIH I. L. G. Nurtjahjaningsih; AYPBC Widyatmoko; Liliek Haryjanto; Yuliah Yuliah; Yayan Hadiyan
Jurnal Pemuliaan Tanaman Hutan Vol 14, No 2 (2020): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2020.14.2.121-128

Abstract

Aquilaria malaccensis (agarwood) is a native species to Indonesia, it produces fragrance. Aim in this study was to assess genetic diversity and structure of A. malaccensis in order to manage seed stand to produce high quality and quantity seeds/seedlings. Leaf samples for DNA template were collected from  populations at western Bangka i.e. Air Gantang, Pelangas, and Simpang Gong. Amplifications of 14 RAPD markers were confirmed; 42 DNA samples of A. malaccensis were analysed using RAPD markers. Parameters of genetic diversity within population i.e. private alleles (PA) and expected heterozygosity (H) were calculated using GeneAlex software. Genetic structure among populations were determined as genetic distance (Da), Analysis Molecular of Variance (AMOVA), a neighbor-joining tree, were calculated using GeneAlex and PopTrew software. Eleven out of 14 RAPD markers were produced 104 stable and polymorphic alleles; PE were found at Air Gantang and Pelangas, 1 and 2 alleles, respectively. The H ranged between 0.133 (Simpang Gong) and 0.328 (Pelangas). The Da was moderate (0.099). AMOVA showed significant genetic diversity among populations (14%). A neighbor-joining tree showed the populations divided into two clusters with high boostrap value, and concordance with their geographical positions. Restricted gene flow might cause a high genetic relationship among populations. Low HEE in Simpang Gong caused this population was priored to being conserved; Air Gantang and Pelangas should be designed as different conservation units due to the population contained different private alleles.
Co-Authors Ari Fiani AYPBC Widyatmoko Hamdan Adma Adinugraha I. L. G. Nurtjahjaningsih Liliek Haryjanto Lukman Hakim Tri Pamungkas Yudohartono Yayan Hadiyan