Article Per Year (5 Year)
p-Index From 2020 - 2025
0.61
P-Index
This Author published in this journals
All Journal JURNAL PEMULIAAN TANAMAN HUTAN
AYPBC Widyatmoko
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Pemuliaan Tanaman Hutan Yogyakarta
Agriculture, Biological Sciences & Forestry

Published : 5 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 5 Documents
Search

 1 
KERAGAMAN GENETIK Taxus Sumatrana DARI POPULASI ALAM DI JAMBI MENGGUNAKAN PENANDA RAPD Istiana Prihatini; Dodi Frianto; Eka Novriyanti; ILG Nurtjahjaningsih; AYPBC Widyatmoko
Jurnal Pemuliaan Tanaman Hutan Vol 15, No 2 (2021): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2021.15.2.95-104

Abstract

The natural population of Taxus sumatrana in several regions in southern Asia including Indonesia has experienced a decline in number. In Sumatra, the population with very few trees was found in Gunung Kerinci and Gunung Tujuh, Kerinci Regency, Jambi. Conservation efforts to protect the extinction of this species are being carried out, one of which is by exploring seeds from natural populations to be planted ex situ. Information on the genetic diversity of Taxus is needed to determine a conservation strategy for this species. This researchwas conducted to obtain information on genetic diversity of Taxus sumatrana. Ten RAPD markers were used to analyze the genetic diversity of 44 individuals from two natural populations, namely Gunung Tujuh and Gunung Kerinci. These markers resulted in 104 loci which were used in the RAPD data analysis using GenAlex ver.6.5 program. The results of the data analysis showed that heterozigosity which showed the level of genetic diversity of the populations of the two populations observed was in moderate level and similar, namely 0.33±0.14 (Gunung Tujuh) and 0.35±0.14 (Gunung Kerinci). The genetic distance between the two populations was also in a moderate value (0.097) and the similarities in genetic structure based on the RAPD markers were showinggenetic variety in moderate level (0.908). The results indicate that the two populations, although geographically close have moderate level of genetic structure. Genetic conservation efforts for future Taxus species could increase the number of individuals from both locations and separate them as two distinct populations.
KAJIAN KERAGAMAN GENETIK ANDALAS (MORUS MACROURA) UNTUK KONSERVASI GENETIK FLORA ASLI SUMATERA BARAT Istiana Prihatini; AYPBC Widyatmoko; Maryatul Qiptiyah; Dodi Frianto; Eka Novriyanti
Jurnal Pemuliaan Tanaman Hutan Vol 15, No 2 (2021): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2021.15.2.65-76

Abstract

Conservation management strategies for native Sumatran species such as andalas (Morus macroura) are currently being developed through genetic diversity studies. This genetic diversity information will be used to enhance ongoing conservation and regeneration programs and to control logging of this high value plant species. The aims of this initial study were to develop a method for extracting DNA from andalas cambium, to select polymorphic RAPD primers and to study genetic diversity using selected RAPD primers. DNA was successfully extracted using the CTAB method from all 32 cambium samples collected from Jambi and West Sumatra. Eighteen RAPD primers were screened and six polymorphic primers selected (OPO-6, OPY-6, OPY15, OPW-1, OPW-3 and OPW-4). RAPD analysis using the six selected primers resulted in 52 polymorphic loci and the value of genetic diversity within the population (Hs) of 0.308 and the value of genetic diversity among populations (DST) of 0.091. The highest genetic diversity was observed in Batipuah, Tanah Datar (0.351) and the lowest was observed in Halaban, Lima Puluh Kota (0.205). A test with AMOVA shows that the genetic diversity between individuals is greater than the diversity between populations. The dendogram based on the genetic distance between populations shows that the andalas population in West Sumatra is separated into two groups and both are separated from the Jambi population. The results of this study can be used as a basis for further research on several other andalas populations as well as the basic information for conservation strategies of andalas.
KERAGAMAN GENETIK POPULASI KAYU KUKU (Pericopsis mooniana, (THWAITES)THWAITES) DI HUTAN LAMEDAI BERDASARKAN PENANDA RAPD ILG Nurtjahjaningsih; AYPBC Widyatmoko; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 13, No 1 (2019): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.414 KB) | DOI: 10.20886/jpth.2019.13.1.25-32

Abstract

Vulnerable status of kayu kuku (Pericopsis mooniana) is due to heavily wood harvesting. Aim in this study is to asses genetic diversity and structure of kayu kuku at Lamedai forest, Southeast Sulawesi using RAPD markers. Leaves samples were collected from 4 natural populations at Lamedai forest i.e. Lamedai natural reserve, Lamedai village, Balijaya and Tangketada. The 22 polymorphic loci of 5 RAPD markers were subjected to the genetic analysis, binary data were calculated by GenAlex software to obtain parameters in genetic diversity within population and analysis molecular variant (AMOVA), while PopTree software was used to construct a dendrogram. Results showed that genetic diversity of the 4 populations was in moderate level (mean He: 0.361 ± 0.017) and the values was similar each other. There was no private allele at any populations, indicating that there was no genetic specification. A dendrogram analysis showed that Lamedai natural reserve genetically closed to Tangketada population and Balijaya, while Lamedai was separated into another cluster. AMOVA analysis showed 13% genetic diversity were observed among populations. In conclution, kayu kuku at Lamedai forest might be a large and continuous population in ancient period.
KERAGAMAN GENETIK Aquilaria malaccensis DARI BANGKA BARAT PROVINSI KEPULAUAN BANGKA BELITUNG DAN IMPLIKASINYA UNTUK PENGELOLAAN TEGAKAN BENIH I. L. G. Nurtjahjaningsih; AYPBC Widyatmoko; Liliek Haryjanto; Yuliah Yuliah; Yayan Hadiyan
Jurnal Pemuliaan Tanaman Hutan Vol 14, No 2 (2020): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2020.14.2.121-128

Abstract

Aquilaria malaccensis (agarwood) is a native species to Indonesia, it produces fragrance. Aim in this study was to assess genetic diversity and structure of A. malaccensis in order to manage seed stand to produce high quality and quantity seeds/seedlings. Leaf samples for DNA template were collected from  populations at western Bangka i.e. Air Gantang, Pelangas, and Simpang Gong. Amplifications of 14 RAPD markers were confirmed; 42 DNA samples of A. malaccensis were analysed using RAPD markers. Parameters of genetic diversity within population i.e. private alleles (PA) and expected heterozygosity (H) were calculated using GeneAlex software. Genetic structure among populations were determined as genetic distance (Da), Analysis Molecular of Variance (AMOVA), a neighbor-joining tree, were calculated using GeneAlex and PopTrew software. Eleven out of 14 RAPD markers were produced 104 stable and polymorphic alleles; PE were found at Air Gantang and Pelangas, 1 and 2 alleles, respectively. The H ranged between 0.133 (Simpang Gong) and 0.328 (Pelangas). The Da was moderate (0.099). AMOVA showed significant genetic diversity among populations (14%). A neighbor-joining tree showed the populations divided into two clusters with high boostrap value, and concordance with their geographical positions. Restricted gene flow might cause a high genetic relationship among populations. Low HEE in Simpang Gong caused this population was priored to being conserved; Air Gantang and Pelangas should be designed as different conservation units due to the population contained different private alleles.
KERAGAMAN GENETIK POPULASI KAYU MERAH (Pterocarpus indicus Willd) MENGGUNAKAN PENANDA RAMDOM AMPLIFIED POLYMORPHISM DNA Purnamila Sulistyawati; AYPBC Widyatmoko
Jurnal Pemuliaan Tanaman Hutan Vol 11, No 1 (2017): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (436.383 KB) | DOI: 10.20886/jpth.2017.11.1.67-76

Abstract

Kayu merah ( Pterocarpus indicus Willd) which listed as one of the IUCN Red List of Threatened Species in the category Vulnerable (VU A1D) is naturally distributed throughout Indonesia included Java, Sulawesi, Maluku, Bali, East Nusa Tenggara, West Nusa Tenggara and Papua. Random Amplified Polymorphic DNA (RAPD) molecular markers have been used to analyze the genetic diversity of six (6) natural populations of kayu merah from Seram Island, Manggarai Timur-Flores, Ngada-Flores, Soe-Timor Tengah Selatan, Kefamenanu-Timor Tengah Utara and Kupang. Ninety-nine (99) polymorphic loci were obtained from twelve RAPD primers. The mean of genetic diversity was 0.2024. Population with the highest genetic diversity was Soe (0.2925) and the lowest genetic diversity was Ngada (0.1212). The highest genetic distance among the populations was between Ngada and Kefamenanu (0.376), while the nearest genetic distance was between Ngada and Manggarai Timur (0.060). The informations resulted from this study are important to support the tree improvement and conservation programme of kayu merah.
Co-Authors Anto Rimbawanto Dodi Frianto Dodi Frianto Eka Novriyanti Eka Novriyanti I. L. G. Nurtjahjaningsih ILG Nurtjahjaningsih ILG Nurtjahjaningsih Istiana Prihatini Liliek Haryjanto Maryatul Qiptiyah Purnamila Sulistyawati Yayan Hadiyan Yuliah Yuliah