Yayan Hadiyan
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Pemuliaan Tanaman Hutan Yogyakarta

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SELEKSI DAN PEROLEHAN GENETIK PADA UJI KETURUNAN NYAWAI (Ficus variegata Blume) DI BANTUL Liliek Haryjanto; Prastyono Prastyono; Yayan Hadiyan
Jurnal Pemuliaan Tanaman Hutan Vol 12, No 2 (2018): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (757.764 KB) | DOI: 10.20886/jpth.2018.12.2.95-104

Abstract

Nyawai (Ficus variegata Blume) is a fast growing species which is promising for forest industrial plantation. Tree improvement of nyawai was then initiated through some progeny trials involving wide range of genetic base in Bantul. Study on initial growth reported that nyawai originated from Cilacap-Pangandaran showed higher genetic variation than those from other locations. However, further growth performance including the effect of selection in the progeny trial was not reported. This study was aimed to observe the growth and genetic parameter of nyawai in the progeny trial at advanced age. The genetic gain resulted from series of within plot selection was also estimated. The design of progeny trial was a randomized complete block with 19 families from Cilacap-Pangandaran, 4 non-contiguous tree-plot, 7 blocks at spacing of 5 m × 5 m. The observed traits were height, diameter at breast height and volume at four years of age. The results of study showed that survival rate was high at 89%. The mean annual increment for height, diameter and volume were 1.52 m/yr; 2.35 cm/yr and 8.65(×10-3) m3/yr, respectively. The proportion of variance to the total variance for family, plot and within plot ranged from 0.06% to 2.10%, 25.54% to 27.50% and 70.57% to 73.91%, respectively. In general, narrow-sense heritability for individual, family and within family were low. Genetic gain from within family selection that was practiced as within plot selection using selection ratio 25% were also low ranging from 0.19% to1.91% for all traits.
VARIASI KANDUNGAN KIMIA MINYAK CENDANA (Santalum album Linn) DARI BERBAGAI PROVENANS DI INDONESIA Liliek Haryjanto; Titis Budi Widowati; Sumardi Sumardi; Ari Fiani; Yayan Hadiyan
Jurnal Pemuliaan Tanaman Hutan Vol 11, No 1 (2017): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (603.027 KB) | DOI: 10.20886/jpth.2017.11.1.77-85

Abstract

The quality of sandalwood (Santalum album Linn) oil primarily depends on the compound of two major sesquiterpenes (α-santalol and β-santalol). The aim of this research was to identify variation of chemical compounds of sandalwood oil from various provenances. Fifty four samples from 8 provenances and one land race taken from 13th years old sandalwood planted at Gunungkidul, Yogyakarta Indonesia. Sandalwood powder were obtained by drilling tree at 10 cm above the ground horizontally. The samples were extracted with n-hexane solvent. Identification of chemical compounds of this essential oils were conducted by gas chromatography mass spectrometry analysis (GCMS). The GCMS investigation of sandalwood oils showed that variation compounds both among individual within provenance and between provenances were found in 11 individual from 6 provenances. Compounds such as sesquiterpene, monoterpenes, and others, were found in variying amount. The results showed that α-santalol (32.38-78.29%), β-santalol (5.45%-37.83%) were the major compounds with varying quantities of compounds range of epi-β-santalol (5.97-17.16%), P-Menth-2-En-9-OL-Trans (2.87%-8.53%), and α-sinensal (3.83-9.39%).
KERAGAMAN GENETIK Aquilaria malaccensis DARI BANGKA BARAT PROVINSI KEPULAUAN BANGKA BELITUNG DAN IMPLIKASINYA UNTUK PENGELOLAAN TEGAKAN BENIH I. L. G. Nurtjahjaningsih; AYPBC Widyatmoko; Liliek Haryjanto; Yuliah Yuliah; Yayan Hadiyan
Jurnal Pemuliaan Tanaman Hutan Vol 14, No 2 (2020): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2020.14.2.121-128

Abstract

Aquilaria malaccensis (agarwood) is a native species to Indonesia, it produces fragrance. Aim in this study was to assess genetic diversity and structure of A. malaccensis in order to manage seed stand to produce high quality and quantity seeds/seedlings. Leaf samples for DNA template were collected from  populations at western Bangka i.e. Air Gantang, Pelangas, and Simpang Gong. Amplifications of 14 RAPD markers were confirmed; 42 DNA samples of A. malaccensis were analysed using RAPD markers. Parameters of genetic diversity within population i.e. private alleles (PA) and expected heterozygosity (H) were calculated using GeneAlex software. Genetic structure among populations were determined as genetic distance (Da), Analysis Molecular of Variance (AMOVA), a neighbor-joining tree, were calculated using GeneAlex and PopTrew software. Eleven out of 14 RAPD markers were produced 104 stable and polymorphic alleles; PE were found at Air Gantang and Pelangas, 1 and 2 alleles, respectively. The H ranged between 0.133 (Simpang Gong) and 0.328 (Pelangas). The Da was moderate (0.099). AMOVA showed significant genetic diversity among populations (14%). A neighbor-joining tree showed the populations divided into two clusters with high boostrap value, and concordance with their geographical positions. Restricted gene flow might cause a high genetic relationship among populations. Low HEE in Simpang Gong caused this population was priored to being conserved; Air Gantang and Pelangas should be designed as different conservation units due to the population contained different private alleles.
KERAGAMANAN GENETIK DAN PERMUDAAN ALAM PUSPA (Schima wallichii (DC.) Korth.) DI TAMAN NASIONAL GUNUNG MERAPI PASCA ERUPSI MERAPI 2010 Bangun Baramantya; Sapto Indrioko; Lies Rahayu Wijayanti Faida; Yayan Hadiyan
Jurnal Pemuliaan Tanaman Hutan Vol 10, No 2 (2016): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (832.061 KB) | DOI: 10.20886/jpth.2016.10.2.111-121

Abstract

Schima wallichii (DC.) Korth. (puspa) is a native vegetation of Gunung Merapi National Park (GMNP). This species has an important function for soil and water conservation in GMNP area, especially in the area with high slope. Puspa is also one of prioritised species planted in the forest ecosystem restoration project of GMNP after 2010 volcano eruption. Information on genetic diversity and natural regeneration potential of each puspa stand groups is then very essential for the success of forest ecosystem restoration in GMNP. Genetic diversity parameters of puspa is assessed by isozyme analysis, while its natural regeneration is evaluated by vegetation analysis. Selection of samples on stand groups of puspa for genetic diversity and natural regeneration analysis is based on levels of vegetation damage (low and moderate) and the planting plots of ecosystem restoration area. The results showed that the stands with low damage level located at Gunung Malang and Mriyan have higher genetic diversity than those with moderate damage level (Balerante and Tlogolele). Stand with the highest genetic diversity is Gunung Malang, with the mean observed heterozigosities (Ho) of 0.284 and minimum fixation index value (FIS) of 0.0508. Stand in the Ecosystem Restoration Demonstration Plot has the lowest level of genetic diversity (Ho = 0.1936; FIS = 0.1127). Regarding natural regeneration potential, the stand group of Gunung Malang is the highest.