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All Journal JURNAL PEMULIAAN TANAMAN HUTAN
Istiana Prihatini
Center for Forest Biotechnology and Tree Improvement Research and Development Yogyakarta
Agriculture, Biological Sciences & Forestry

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KERAGAMAN GENETIK Taxus Sumatrana DARI POPULASI ALAM DI JAMBI MENGGUNAKAN PENANDA RAPD Istiana Prihatini; Dodi Frianto; Eka Novriyanti; ILG Nurtjahjaningsih; AYPBC Widyatmoko
Jurnal Pemuliaan Tanaman Hutan Vol 15, No 2 (2021): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2021.15.2.95-104

Abstract

The natural population of Taxus sumatrana in several regions in southern Asia including Indonesia has experienced a decline in number. In Sumatra, the population with very few trees was found in Gunung Kerinci and Gunung Tujuh, Kerinci Regency, Jambi. Conservation efforts to protect the extinction of this species are being carried out, one of which is by exploring seeds from natural populations to be planted ex situ. Information on the genetic diversity of Taxus is needed to determine a conservation strategy for this species. This researchwas conducted to obtain information on genetic diversity of Taxus sumatrana. Ten RAPD markers were used to analyze the genetic diversity of 44 individuals from two natural populations, namely Gunung Tujuh and Gunung Kerinci. These markers resulted in 104 loci which were used in the RAPD data analysis using GenAlex ver.6.5 program. The results of the data analysis showed that heterozigosity which showed the level of genetic diversity of the populations of the two populations observed was in moderate level and similar, namely 0.33±0.14 (Gunung Tujuh) and 0.35±0.14 (Gunung Kerinci). The genetic distance between the two populations was also in a moderate value (0.097) and the similarities in genetic structure based on the RAPD markers were showinggenetic variety in moderate level (0.908). The results indicate that the two populations, although geographically close have moderate level of genetic structure. Genetic conservation efforts for future Taxus species could increase the number of individuals from both locations and separate them as two distinct populations.
KAJIAN KERAGAMAN GENETIK ANDALAS (MORUS MACROURA) UNTUK KONSERVASI GENETIK FLORA ASLI SUMATERA BARAT Istiana Prihatini; AYPBC Widyatmoko; Maryatul Qiptiyah; Dodi Frianto; Eka Novriyanti
Jurnal Pemuliaan Tanaman Hutan Vol 15, No 2 (2021): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2021.15.2.65-76

Abstract

Conservation management strategies for native Sumatran species such as andalas (Morus macroura) are currently being developed through genetic diversity studies. This genetic diversity information will be used to enhance ongoing conservation and regeneration programs and to control logging of this high value plant species. The aims of this initial study were to develop a method for extracting DNA from andalas cambium, to select polymorphic RAPD primers and to study genetic diversity using selected RAPD primers. DNA was successfully extracted using the CTAB method from all 32 cambium samples collected from Jambi and West Sumatra. Eighteen RAPD primers were screened and six polymorphic primers selected (OPO-6, OPY-6, OPY15, OPW-1, OPW-3 and OPW-4). RAPD analysis using the six selected primers resulted in 52 polymorphic loci and the value of genetic diversity within the population (Hs) of 0.308 and the value of genetic diversity among populations (DST) of 0.091. The highest genetic diversity was observed in Batipuah, Tanah Datar (0.351) and the lowest was observed in Halaban, Lima Puluh Kota (0.205). A test with AMOVA shows that the genetic diversity between individuals is greater than the diversity between populations. The dendogram based on the genetic distance between populations shows that the andalas population in West Sumatra is separated into two groups and both are separated from the Jambi population. The results of this study can be used as a basis for further research on several other andalas populations as well as the basic information for conservation strategies of andalas.
Co-Authors AYPBC Widyatmoko Dodi Frianto Dodi Frianto Eka Novriyanti Eka Novriyanti ILG Nurtjahjaningsih Maryatul Qiptiyah