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All Journal JURNAL PEMULIAAN TANAMAN HUTAN
ILG Nurtjahjaningsih
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Pemuliaan Tanaman Hutan Yogyakarta
Agriculture, Biological Sciences & Forestry

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KEKERABATAN GENETIK ANAKAN ALAM ULIN (Eusideroxylon zwageri TEIJSM. & BINN.) MENGGUNAKAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA ILG Nurtjahjaningsih; Sukartiningsih Sukartiningsih; Anna Puspa Amarta Saranti; Purnamila Sulistyawati; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 11, No 1 (2017): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (411.592 KB) | DOI: 10.20886/jpth.2017.11.1.25-32

Abstract

The study aimed to assess genetic diversity and genetic relationship of ulin wildlings randomly collected from a nursery and originated from Bukit Soeharto natural forest, East Kalimantan. DNA templates were extracted from leaf samples of 1.5 years old wildings. Five RAPD primers consisted 55 polymorphic loci were used for genetic studies. Genetic diversity and relationship were analyzed using GenAlex software. The results showed moderate mean value of genetic diversity (HE=0,345, SE 0,015) of the wildings. Forty eight wildings were clustered in only 3 groups; almost all wildings (65%) were clustered in one main cluster. Moreover, 4 wildlings were clones (8%). In conclusion, the 48 wildings of ulin consisted high genetic relationship and individual clones that reflects the low genetic diversity of this species.
KERAGAMAN GENETIK Taxus Sumatrana DARI POPULASI ALAM DI JAMBI MENGGUNAKAN PENANDA RAPD Istiana Prihatini; Dodi Frianto; Eka Novriyanti; ILG Nurtjahjaningsih; AYPBC Widyatmoko
Jurnal Pemuliaan Tanaman Hutan Vol 15, No 2 (2021): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2021.15.2.95-104

Abstract

The natural population of Taxus sumatrana in several regions in southern Asia including Indonesia has experienced a decline in number. In Sumatra, the population with very few trees was found in Gunung Kerinci and Gunung Tujuh, Kerinci Regency, Jambi. Conservation efforts to protect the extinction of this species are being carried out, one of which is by exploring seeds from natural populations to be planted ex situ. Information on the genetic diversity of Taxus is needed to determine a conservation strategy for this species. This researchwas conducted to obtain information on genetic diversity of Taxus sumatrana. Ten RAPD markers were used to analyze the genetic diversity of 44 individuals from two natural populations, namely Gunung Tujuh and Gunung Kerinci. These markers resulted in 104 loci which were used in the RAPD data analysis using GenAlex ver.6.5 program. The results of the data analysis showed that heterozigosity which showed the level of genetic diversity of the populations of the two populations observed was in moderate level and similar, namely 0.33±0.14 (Gunung Tujuh) and 0.35±0.14 (Gunung Kerinci). The genetic distance between the two populations was also in a moderate value (0.097) and the similarities in genetic structure based on the RAPD markers were showinggenetic variety in moderate level (0.908). The results indicate that the two populations, although geographically close have moderate level of genetic structure. Genetic conservation efforts for future Taxus species could increase the number of individuals from both locations and separate them as two distinct populations.
STRUKTUR GENETIK Calliandra calothyrsus DI INDONESIA MENGGUNAKAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA (RAPD) ILG Nurtjahjaningsih; Purnamila Sulistyawati; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 10, No 1 (2016): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2016.10.1.31-38

Abstract

Calliandra calothyrsus was introduced to Indonesia from Guatemala in 1930s and had been planted widely throughout Indonesia for fuel wood. Genetic diversity within and among population is an important factor for initiating breeding program. Aim in this study was to evaluate genetic structure of C. calothyrsus in Indonesia, to obtain a better understanding of the history of the distribution and efficiency of breeding strategy of this species. Leaf samples were collected in a seedling seed orchard plot from 10 populations. Six RAPD markers consisted of 34 loci were used to analyze genetic diversity and genetic structure. The results showed that genetic diversity was in low to moderate level (mean HE= 0.321). The AMOVA analysis showed that genetic differentiation among geographical sources was significant, also among populations within geographical sources and among individual within populations as well. It was revealed that the 10 populations originated from only two ancestors. The limited number of ancestors resulted low to moderate genetic distances among populations (mean Da=0.070). In conclusion, C. calothyrsus has moderate genetic diversity within population and weak genetic structure among populations. Genetic diversity in population/individual level should be considered as a selection unit in the breeding strategies.
Co-Authors Anna Puspa Amarta Saranti Anto Rimbawanto AYPBC Widyatmoko Dodi Frianto Eka Novriyanti Istiana Prihatini Purnamila Sulistyawati Sukartiningsih Sukartiningsih