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All Journal Vegetalika JURNAL PEMULIAAN TANAMAN HUTAN Tropical Plantation Journal
Etty Handayani
Universitas Muhammadiyah Yogyakarta
Agriculture, Biological Sciences & Forestry

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PENGARUH BERBAGAI KONSENTRASI GLUTAMIN TERHADAP PERTUMBUHAN TUNAS Aquilaria malaccensis Lamk. SECARA KULTUR JARINGAN Chrisnanda Ayu Melati; Etty Handayani; Toni Herawan
Jurnal Pemuliaan Tanaman Hutan Vol 15, No 2 (2021): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2021.15.2.145-151

Abstract

Aquilaria malaccensis Lamk. is a type of plant that is threatened with extinction due to the high level of illegal collection from the forest. Tissue culture techniques provide an alternative to vegetative propagation on a large scale for conservation efforts. The addition of glutamine to the culture media was intended to increase the growth of A. malaccensis Lamk shoots. The research was conducted with quantitative research methods using single factor laboratory experiments with four (4) treatments arranged in a completely randomized design (CRD). The treatments were differentiated based on the addition of glutamine, namely, G0: 0 mg / l; G1: 10 mg / l; G2: 20 mg / l; G3: 30 mg / l. Data analysis used Analysis of Variance (Annova) with a level of α = 5%. The results showed that Glutamine could't affect the growth of A. malaccensis Lamk, but there was the best trend in the addition of Glutamine 20 mg/l.
Keragaman Genetik 27 Aksesi Kedelai (Glycine max L. Merr.) Introduksi Subtropis berdasarkan Marka SSR Puji Lestari; Rizki Eka Putri; Innaka Ageng Rineksane; Etty Handayani; Kristianto Nugroho; Rerenstradika Tizar Terryana
Vegetalika Vol 10, No 1 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.58418

Abstract

Karakterisasi aksesi kedelai (Glycine max L. Merr.) dalam koleksi perlu dilakukan berdasarkan marka molekuler untuk mendukung karakter morfologi. Penelitian ini bertujuan untuk menganalisis keragaman genetik aksesi kedelai yang diintroduksi dari daerah subtropis menggunakan marka simple sequence repeats (SSR) yang didukung melalui informasi karakter morfologi. Hasil penelitian menunjukkan bahwa sebanyak 15 SSR berhasil dideteksi polimorfismenya pada 27 aksesi kedelai dari daerah subtropis dengan total 158 alel berukuran antara 100-368 bp dengan kisaran jumlah 4-18 alel per lokus. Rata-rata polymorphism information content (PIC) ditemukan sebesar 0,92 dengan nilai tertinggi 0,96 (SATT463, SATT249, SATT063) dan nilai terendah 0,87 (SATT038). Semua marka SSR memiliki nilai PIC >0,8 yang mengindikasikan sebagai marka sangat informatif, artinya mampu mendiferensiasi antar aksesi dan dapat diaplikasikan dalam mendeteksi keragaman genetik plasma nutfah kedelai lainnya. Keragaman genetik aksesi kedelai terebut sangat tinggi seperti yang direfleksikan oleh rata-rata indeks diversitas gen sebesar 0,93. Analisis klaster dengan marka SSR berhasil membagi 27 aksesi kedelai menjadi dua kelompok utama yang sebagian besar dalam kelompok I (26 aksesi) dan kelompok II khusus aksesi D76-8070. Karakterisasi molekuler dengan SSR tersebut mendukung keragaman yang tinggi karakter morfologi yang memisahkan total aksesi menjadi empat kuadran. Informasi keragaman genetik berdasarkan marka S yang didukung karakter morfologi tersebut dapat menjadi dasar awal seleksi tetua persilangan aksesi dari daerah subtropis untuk pengembangan varietas baru melalui pemuliaan kedelai di iklim tropis Indonesia.
In Vitro Culture of Orchids Grammatophyllum scriptum lindl. from Ambonese banana Peel in Foliar Fertilizer Medium Etty Handayani; Agung Astuti; Yogawati Printarani Yahwidhi
Tropical Plantation Journal Vol 2, No 2 (2023): TROPICAL PLANTATION JOURNAL
Publisher : Akademi Komunitas Perkebunan Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56125/tpj.v2i2.31

Abstract

The sustainability of Grammatophyllum scriptum  orchids in nature is threatened due to excessive harvesting which results in changes and damage to the orchid's growing habitat. G. scriptum  orchid is one of the endemic orchids that has uniqueness and high economic value. In vitro culture propagation  of G. scriptum orchids  is carried out to multiply and maintain the existence of the orchid. The use of alternative media  in vitro culture  is carried out to minimize the cost of making the medium and utilize materials available in nature. The utilization of Ambonese banana peel waste that has not been carried out optimally is considered to be one of the choices as an alternative medium in propagation  of G. scriptum  orchids This study aims to determine the most appropriate combination of foliar fertilizer and banana peel concentration for in vitro multiplication of orchid explants and to  examine the effect of the combination of leaf fertilizer and banana peel concentration on the multiplication of orchid explantsVitro. The study will be carried out using a single-factor experimental method with 8 treatments, arranged according to the Complete Randomized Design (RAL). The treatment tried was in vitro culture of G. scriptum  orchids  on Growmore media  and Ambonese banana peels including inner banana peels of 50g/L and 100g/L; outer banana peel 50 g/L and 100 g/L; banana peel combined 50 g / L and 100 g / L. The results showed the addition of Ambon banana peel had an influence on the percentage of life and growth of shoots,   leaves and roots of G. scriptum  orchid plants Treatment with the addition of a combined banana peel of 50 g / L in leaf fertilizer medium provides the best growth in terms of the percentage of life, plant height growth, leaves and plant roots.
Substitution Media by Utilizing Banana Peel for In Vitro Culture of Chrysanthemum morifolium Etty Handayani; Innaka Ageng Rineksane; Supriyatin Kartika Sari; Fany Permatasari
Tropical Plantation Journal Vol 5, No 1 (2026): TROPICAL PLANTATION JOURNAL
Publisher : Akademi Komunitas Perkebunan Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56125/tpj.v5i1.68

Abstract

The use of organic matter in the in vitro culture medium can reduce costs for micropropagation and multiplication of Chrysanthemum in mass production. This study aims to examine the use of banana peel waste in the in vitro culture of Chrysanthemum. The research used an experimental method with single factor and eight treatments with 3 samples and 3 replications. The treatments were: (1) MS medium +  BAP 1 ppm + NAA 0.5 ppm, (2) Foliar fertilizer 3 g/L +  coconut water 150 mL/L, (3) banana endocarp puree 50 g/L, (4)  banana endocarp puree 100 g/L, (5)  banana exocarp puree 50 g/L, (6) banana exocarp puree 100 g/L, (7) banana peel puree 100 g/L, and (8) banana peel puree 100 g/L. All banana peel puree treatments were combined with foliar fertilizer 3g/L + coconut water 150 mL/L. The explant used a single nodus of Chrysanthemum with one leaf resulting from the previous in vitro culture. This study used Chrysanthemum varieties Naweswari and Tadasita from Ornamental Plants Research Center, West Java, Indonesia. The results showed that foliar fertilizer medium added by banana peel puree and coconut water has the good potential to be used as a substitution medium in the in vitro culture of Chrysanthemum varieties Naweswari and Tadasita. The banana peel puree  50 g/L in the foliar fertilizer medium shows the best growth in Chrysanthemum in vitro culture in both Naweswari and Tadasita varieties.
Co-Authors Agung Astuti Chrisnanda Ayu Melati Fany Permatasari Innaka Ageng Rineksane Kristianto Nugroho Puji Lestari Rerenstradika Tizar Terryana Rizki Eka Putri Supriyatin Kartika Sari Toni Herawan Yogawati Printarani Yahwidhi