Article Per Year (5 Year)
p-Index From 2020 - 2025
0.23
P-Index
This Author published in this journals
All Journal JURNAL PEMULIAAN TANAMAN HUTAN
Toni Herawan
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Pemuliaan Tanaman Hutan Yogyakarta
Agriculture, Biological Sciences & Forestry

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
PENGARUH BERBAGAI KONSENTRASI GLUTAMIN TERHADAP PERTUMBUHAN TUNAS Aquilaria malaccensis Lamk. SECARA KULTUR JARINGAN Chrisnanda Ayu Melati; Etty Handayani; Toni Herawan
Jurnal Pemuliaan Tanaman Hutan Vol 15, No 2 (2021): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2021.15.2.145-151

Abstract

Aquilaria malaccensis Lamk. is a type of plant that is threatened with extinction due to the high level of illegal collection from the forest. Tissue culture techniques provide an alternative to vegetative propagation on a large scale for conservation efforts. The addition of glutamine to the culture media was intended to increase the growth of A. malaccensis Lamk shoots. The research was conducted with quantitative research methods using single factor laboratory experiments with four (4) treatments arranged in a completely randomized design (CRD). The treatments were differentiated based on the addition of glutamine, namely, G0: 0 mg / l; G1: 10 mg / l; G2: 20 mg / l; G3: 30 mg / l. Data analysis used Analysis of Variance (Annova) with a level of α = 5%. The results showed that Glutamine could't affect the growth of A. malaccensis Lamk, but there was the best trend in the addition of Glutamine 20 mg/l.
PENGUJIAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA UNTUK MENGETAHUI KESTABILAN GENETIK KLON JATI (Tectona grandis) ILG Nurtjahjaningsih; Toni Herawan; Reza Permatasari Rachma; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 12, No 2 (2018): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2018.12.2.127-134

Abstract

This study aimed to test RAPD markers to assess genetic stability of teak clones. Two experimental steps were carried out. First, nine RAPD markers were screened to verify the level of polymorphic loci; second, the polymorphic loci were applied to test genetic stability of clones. To test polymorphism levels of the primers, DNA was isolated from eight leaf samples that were collected from a seed orchard located at Watusipat, Gunung Kidul. To verify genetic stability of clones, DNA was isolated from leaf samples of 24 ramets of 3 clones after second sub-culturing. Results showed that amplification of 5 out of 9 RAPD primers were be consisten and produced 12 polymorphic loci. The number of polymorphic alleles per locus ranged between 1 and 3; the allele sizes were between 400 and 1,050 base pairs (bps). The percentage of polymorphic loci was 100%; it meant that overall loci have high polymorphism level. Based on these loci showed that the 24 ramets are clones; there was no somaclonal variation or high genetic stability. However, these loci need to be validated using more stable DNA markers.
REGENERASI PERAKARAN PLANTLET IN VITRO DAN EX VITRO PADA KULTUR JARINGAN CENDANA (Santalum album) Asri Insiana Putri; Toni Herawan
Jurnal Pemuliaan Tanaman Hutan Vol 12, No 2 (2018): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2018.12.2.143-150

Abstract

Cendana (Santalum album Linn.) is one of the important hemiparasite species due to its high value essential oil for pharmaceutical industries. However, since1998 this species has been categorized as vulnerable by the IUCN Red List. The propagation of cendana has been hampered by inadequacy in regeneration, either through sexual or vegetative propagation. Regeneration of cendana through in vitro technique is still limited due to the difficulty in rooting and acclimatization. The purpose of this study is to observe the effect of clones, in vitro and ex vitro techniques on the primary and secondary root development. Two clones of cendana: Clone A.III.4.14 and WS28 were tested in Gresshoff & Doy culture media enriched by IBA 20 mg/l; IAA 0.15 mg/l and kinetin 0.15 mg/l. Root development was observed for six months of culture for in vitro and three months after acclimatization in a greenhouse for ex vitro. The results of this study showed that Clone A.III.4.14 formed primary root in lower percentage rate (41.85%) than Clones WS28 (60,44%), on the contrary it grew secondary root in higher percentage rate (58.15%) than Clone WS28 (39,56%). The ex vitro following the acclimatization showed that the root hairs grew only in the plantlets which formed secondary root during in vitro. This result indicates an important of clone’s selection for secondary root development during in vitro to obtain a better root system in the success of acclimatization of cendana.
Co-Authors Anto Rimbawanto Asri Insiana Putri Chrisnanda Ayu Melati Etty Handayani ILG Nurtjahjaningsih Reza Permatasari Rachma