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All Journal JURNAL PEMULIAAN TANAMAN HUTAN
AYPBC Widyatmoko
Balai Besar Penelitian Bioteknologi dan Pemuliaan Tanaman Hutan
Agriculture, Biological Sciences & Forestry

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Variasi Genetik Pinus merkusii Menggunakan Penanda Mikrosatelit Kloroplas ILG Nurtjahjaningsih; AYPBC Widyatmoko; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 5, No 3 (2011): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2011.5.3.119-128

Abstract

Using two chloroplast universal primers, haplotipic within and among populations of Pinus merkusii had been evaluated, in order to access paternal variation of this species. The aim of this study was to investigate variation of chloroplast microsatellite in P. merkusii. Using two universal chloroplast microsatelites, a total of 18 chloroplast haplotypes were found among 730 individuals surveyed in 10populations in Java. Private haplotypes mostly found at eastern Java populations. The number ofdetected haplotypes (Na) within populations ranged between 5 and 14. Variation of haplotypes (h) was relatively hight; it ranged between 0.727 and 0.858. Nei’s genetic distance among populations was very low (Da=0.030). Moreover, analysis of molecular variance (AMOVA) showed genetic variation originated from within population but the value was insignificant.It was concluded that private haplotypes found in eastern Java might show gene pool of P. merkusii in Java. These detected haplotypes are of important information for genetic resources conservation anf breeding strategy of P. merkusii.
IDENTIFIKASI Aquilaria malaccensis DAN A. microcarpa MENGGUNAKAN PENANDA RAPD Anto Rimbawanto; AYPBC Widyatmoko
Jurnal Pemuliaan Tanaman Hutan Vol 5, No 1 (2011): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2011.5.1.23-30

Abstract

Aquilaria sp, belong to Family Thymelaeaceae, is one of several species whose can produce agarwood or gaharu. Quality of gaharu of this species is better than the other species. With high economic value, demand of Aquilaria sp increase and also increase exploitation of the species without counterbalanced by enough planting activity. Therefore, conservation and breeding program of the species become important to carry out. Two important Aquilaria sp.  are A. malaccensis and A. microcarpa. However, both species only can be distinguished by generative characteristics such as flower and fruit. The most appropriate and rapid method to identify species is DNA marker, especially using RAPD markers. In order to obtain DNA marker to species identification, 2 selection steps should be carried out. The first step was using 2-3 samples per species. From 180 RAPD primers, only 5 primers produced loci those can identify both species, those are D-20, A-17, G-12, R-15 and U-13. The second selection step was carried out by increasing number of samples per species (24 samples in total). From the five primers obtained from first selection, only U-13 700 basepairs that can be selected as species-specific DNA markers to identify both Aquilaria sp.
Co-Authors Anto Rimbawanto ILG Nurtjahjaningsih