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All Journal JURNAL PEMULIAAN TANAMAN HUTAN
Anto Rimbawanto
Balai Besar Penelitian Bioteknologi dan Pemuliaan Tanaman Hutan
Agriculture, Biological Sciences & Forestry

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Karakterisasi Morfologi dan verifikasi DNA Ganoderma philippii penyebab busuk akar Acacia mangium Desy Puspitasari; Anto Rimbawanto; Nur Hidayati
Jurnal Pemuliaan Tanaman Hutan Vol 3, No 2 (2009): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1342.018 KB) | DOI: 10.20886/jpth.2009.3.2.83-94

Abstract

The incidence of rot disese has inflicted substantial damage to some plantation of Acacia mangium in Sumatera and Kalimantan. As soil-borne pathogen, Ganoderma is commonly found in the tropic and in Indonesia the disease also couse significacant losses to rubber and soil palm plantation. To help control the disease, appropriate identification of the fungi cousing the disease and other field characteritics are critical. Morphological characteistics applied in this study has proved to be effective to identify the pathogen. The disease is predominantly caused by G. philippii. The result of morphological identicication was verified with the DNA sequence of the same fungus and confirm the accuracy of the morphological characteristics. Other fungi that attack root of Acacia mangium are G. australe and Phellinus spp.
KARAKTERISTIK PEMBUNGAAN DAN SISTEM PERKAWINAN NYAMPLUNG (Calophyllum inophyllum) PADA HUTAN TANAMAN DI WATUSIPAT, GUNUNG KIDUL ILG Nurtjahjaningsih; Purnamila Sulistyawati; AYPBC Widyatmoko; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 6, No 2 (2012): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2012.6.2.65-78

Abstract

Flowering are influenced by internal factors, such as genetic and phytohormone, and environment factors, such as sunlight and nutrition intake. The flowering characteristics influence fruiting and genetic diversity seedlings through mating systems. This study aims to assess flowering and fruiting characteristics and to determine pattern of mating system of a Calophyllum inophyllum plantation at Watusipat, Gunung Kidul. Flowering and fruiting were observed at 4 locations, 3 parts of crown, and 4 main directions to know the effects of sunlight, nutrition intake and phytohormone in the flowering process. Mating system was assessed by comparing genetic diversity values between parent trees and offsprings. The values of genetic diversity were analyzed using 5 RAPD primers with 17 polymorphic loci. Analysis of variant showed that the locations, crown parts, directions and interaction between a location and direction significantly affected to differences number of flowers and fruits. Values of genetic diversity (h) of parent trees ranged between 0.1471 and 0.3056. The values increased at almost overall offsprings; it ranged between 0.2864 and 0.3750. Values of genetic distance (Da) between parent trees were high and very high (0.197 – 0.364), but the values was decreased between parent trees and their offspring, even between offspring populations. A dendrogram showed two main clusters; first cluster consisted parent trees at up edge with rare trees and second cluster consisted sub cluster parent trees at up edge; sub cluster parent trees at down middle; and sub cluster parent trees at down edge and overall offsprings. Flowering/ fruiting characteristics and pattern of mating systems of C. inophyllum were briefly discussed. Key words : Flowering characteristic, genetic diversity, Calophyllum inophyllum, RAPD analysis
Variasi Genetik Pinus merkusii Menggunakan Penanda Mikrosatelit Kloroplas ILG Nurtjahjaningsih; AYPBC Widyatmoko; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 5, No 3 (2011): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2011.5.3.119-128

Abstract

Using two chloroplast universal primers, haplotipic within and among populations of Pinus merkusii had been evaluated, in order to access paternal variation of this species. The aim of this study was to investigate variation of chloroplast microsatellite in P. merkusii. Using two universal chloroplast microsatelites, a total of 18 chloroplast haplotypes were found among 730 individuals surveyed in 10populations in Java. Private haplotypes mostly found at eastern Java populations. The number ofdetected haplotypes (Na) within populations ranged between 5 and 14. Variation of haplotypes (h) was relatively hight; it ranged between 0.727 and 0.858. Nei’s genetic distance among populations was very low (Da=0.030). Moreover, analysis of molecular variance (AMOVA) showed genetic variation originated from within population but the value was insignificant.It was concluded that private haplotypes found in eastern Java might show gene pool of P. merkusii in Java. These detected haplotypes are of important information for genetic resources conservation anf breeding strategy of P. merkusii.
IDENTIFIKASI Aquilaria malaccensis DAN A. microcarpa MENGGUNAKAN PENANDA RAPD Anto Rimbawanto; AYPBC Widyatmoko
Jurnal Pemuliaan Tanaman Hutan Vol 5, No 1 (2011): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2011.5.1.23-30

Abstract

Aquilaria sp, belong to Family Thymelaeaceae, is one of several species whose can produce agarwood or gaharu. Quality of gaharu of this species is better than the other species. With high economic value, demand of Aquilaria sp increase and also increase exploitation of the species without counterbalanced by enough planting activity. Therefore, conservation and breeding program of the species become important to carry out. Two important Aquilaria sp.  are A. malaccensis and A. microcarpa. However, both species only can be distinguished by generative characteristics such as flower and fruit. The most appropriate and rapid method to identify species is DNA marker, especially using RAPD markers. In order to obtain DNA marker to species identification, 2 selection steps should be carried out. The first step was using 2-3 samples per species. From 180 RAPD primers, only 5 primers produced loci those can identify both species, those are D-20, A-17, G-12, R-15 and U-13. The second selection step was carried out by increasing number of samples per species (24 samples in total). From the five primers obtained from first selection, only U-13 700 basepairs that can be selected as species-specific DNA markers to identify both Aquilaria sp.
Co-Authors AYPBC Widyatmoko AYPBC Widyatmoko Desy Puspitasari ILG Nurtjahjaningsih ILG Nurtjahjaningsih Nur Hidayati Purnamila Sulistyawati