Fitriana Fitriana
Fakultas Farmasi, Universitas Muslim Indonesia, Makassar

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ISOLASI FUNGI ENDOFIT KULIT BUAH MERAH (Pandanus conoideus Lam.) YANG BERPOTENSI SEBAGAI ANTIBAKTERI SECARA METODE KLT - BIOAUTOGRAFI Reza Ariyadi Deponda; Fitriana Fitriana; Siska Nuryanti; Herwin Herwin
As-Syifaa Jurnal Farmasi Vol 11, No 2 (2019): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.544 KB) | DOI: 10.33096/jifa.v11i2.583

Abstract

Red fruit (Pandanus conoideus Lam.) belongs to genus Pandanus. Red fruit oil has the activity as an antibacterial. The research aimed to obtain endophytic fungi isolates on the red fruit peel and assay the antibacterial activity by TLC-Bioautography. The research was experimentally conducted including the process of isolation of endophytic fungi, purification, macroscopic examination, screening for antibacterial activity against Staphylococcus aureus and Salmonella typhi, fermentation for 7, 14 and 21 days, separation and antibacterial assay by TLC-Bioautography against Staphylococcus aureus and Salmonella typhi, the isolate fermentate of endophytic fungi for 7 days showed an Rf value of 0.49 against Staphylococcus aureus and an 0.47 against Salmonella typhi. The isolate fermentate of endophytic fungi for 14 days showed an Rf value of 0.58 against Staphylococcus aureus and an 0.69 against Salmonella typhi. The isolate fermentate of endophytic fungi for 21 days showed an Rf value of 0.69 against Staphylococcus aureus and an 0.67 against Salmonella typhi. In conclusion, red fruit peel had endophytic fungi isolate with the potency as an antibacterial.
AKTIVITAS ANTIOKSIDAN EKSTRAK FERMENTAT ISOLAT FUNGI ENDOFIT BIJI BUAH KENARI (Canarium indicum L.) SECARA KLT-AUTOGRAFI Nurul Khatimah Naim; Herwin Herwin; Fitriana Fitriana; Ayyub Harly Nurung
As-Syifaa Jurnal Farmasi Vol 13, No 2 (2021): AS-SYIFAA JURNAL FARMASI
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/jifa.v13i2.780

Abstract

Walnut seeds (Canarium indicum L.) contain tannin, flavonoids, carotenoids which is potentially fungtioning as antioxidants. The research aimed to examine the antioxidant activity of fermentate extract of walnut seeds endophytic fungi by autography-TLC. The result of the endophytic fungi isolation in Walnut seeds (Canarium indicum L.) obtained 5 isolates with characteristics. The test result of screening free antiradical activity obtained that isolate 4th was active as an antioxidant indicated by the presence of brown spots with the addition of 1,1-diphenyl-2-picrylhydrazyl 0.004%. Isolate 4th was fermented using Maltosa Yeast Broth (MYB) medium to obtain fermentate supernatant and mycelia. The Fermentate of supernantant of isolate 4th was extracted to obtained supernantant of ethyl acetate extract. Based on the result of free antiradical activity test of ethyl acetate fermentate extract isolate 4th by autography-TLC using ethyl acetate: methanol: water eluent (6:2:1), obtained Rf 0.78 value active as free antiradical by spraying 1,1diphenyl-2-picrylhydrazil 0.004%.
KARAKTERISASI ISOLAT BAKTERI PENGHASIL SELULOSA DARI BUAH NAGA MERAH (Hylocereus polyrhizus) Siska Nuryanti; Fitriana Fitriana; A. Rini Pratiwi
As-Syifaa Jurnal Farmasi Vol 13, No 1 (2021): AS-SYIFAA JURNAL FARMASI
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/jifa.v13i1.770

Abstract

Cellulose is a natural polymer widely used for general industrial purposes. The development of science utilizes bacteria from certain fruits to produce biosellulose. Red dragon fruit (Hylocereus polyrhizus) contains a lot of sugar which can be bioconverted to cellulose by cellulose- producing lines. The research aimed to obtain bacteria from red dragon fruit with the potential to produce cellulose, then conduct morphological and biochemical characterization. The research was conducted by the isolation of bacteria from the fruit, purification, and screening tests using Hestrin-Schramm medium. The isolates obtained were characterized morphologically through macroscopic and microscopic observation and the biochemically through catalase test, carbohydrate fermentation, starch and gelatin hydrolysis, motility, and IMViC (Indole, Methyl red, Voges- Proskauer, Citrate). The results showed that 4 bacterial isolates had the potential to produce cellulose. Based on Bergey's Manual of Systematic Bacteriology, 3 bacterial genera were obtained, namely Acetobacter, Gluconacetobacter, and Azotobacter. The results of the characteristics of the isolates obtained at IBBN 2-2 were positive in the catalase test, sucrose and lactose fermentation, and methyl red. IBBN 3-1 was positive in the catalase test, sugar fermentation, starch hydrolysis, methyl red, and citrate. IBBN 3-2 was positive in the sucrose and glucose fermentation test, gelatin hydrolysis and 4-1 IBBN was positive in the sucrose fermentation test and gelatin hydrolysis.
PROFIL BIOAUTOGRAM EKSTRAK FERMENTAT ISOLAT FUNGI ENDOFIT DARI DAUN GALING-GALING (Cayratia trifolia L) SEBAGAI ANTIBAKTERI Fitriana Fitriana; As Adi Abdullah; Annisa Almagfirah Achmar
As-Syifaa Jurnal Farmasi Vol 11, No 1 (2019): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (109.159 KB) | DOI: 10.33096/jifa.v11i1.494

Abstract

The research bioautogram profile of fermentate extract endophytic fungi from galing-galing (Cayratia trifolia L) leaf as an antibacterial has been to find the activity of the extract fermentat endophytic fungi isolates against bacterial test using TLC Bioautography. This research begins with the isolation of leaves galing-galing using Potato Dextrose Agar Chloramfenicol (PDAC) media, isolates do purification and macroscopic test. Subsquently isolates were followed by screening endophytic fungi isolates against test bacteria. Isolates that give the most excellent activity followed by fermentation with Maltose Yeast Broth (MYB) media using a shaker speed of 200 rpm at room temperature for 21 days. After the fermented liquid is separated to obtain fermentat and  mycelia. The fermented liquid was then extracted using a liquid-liquid extraction method using ethyl acetate solvents after which it was evaporated with a rotary evaporator until a thick extract was obtained. Extracts obtained followed by TLC Bioautography method using ethyl acetate : n-hexane (4: 1) eluent.  Endophytic fungi were obtained as many as 6 isolates and the best activity was endophytic fungi with IDGG-03 code against test bacteria. The results from the TLC Bioautography profile 2 Bioautography provide active spot with Rf values of 0.09 and 0.54 against the bacteria B. subtilis, V. cholerae and P. aeruginosa.
AKTIVITAS ANTIMIKROBA EKSTRAK ETANOL BATANG WOLE WOE ASAL HALMAHERA TENGAH TERHADAP BAKTERI GRAM POSITIF Staphylococcus epidermidis DAN Staphylococcus aureus Fitriana Fitriana; Sitti Amirah; Safriani Rahman
As-Syifaa Jurnal Farmasi Vol 14, No 1 (2022): AS-SYIFAA JURNAL FARMASI
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jifa.v14i1.793

Abstract

The wole woe plant is one of the plants empirically used by the Weda community in Central Halmahera district as a traditional medicine to treat various diseases such as breast cancer, cysts, vaginal discharge, diabetes mellitus, wounds, dysentery, cholesterol, and gout. Therefore, a study was conducted on the antimicrobial activity of the ethanolic extract of wole woe stems against gram-positive bacteria S. epidermidis and S. aureus with the aim of determining the concentration that provides antimicrobial activity of the ethanolic extract of wole woe stems by using the agar diffusion method. The results obtained in the screening test gave activity at a concentration of 0.1% and the results of the minimum inhibitory concentration test (MIC) and the minimum killing concentration test (MBC) obtained a MIC value of 1.6% for S. epidermidis bacteria and 0.8% for S. aureus bacteria, while the MBC value is 1.6% for both bacteria. The results of statistical tests on the diameter of the inhibition zone from the antimicrobial activity test against S. epidermidis and S. aureus, showed that there was a difference in the ability to inhibit bacterial growth between the positive control and the test concentration group of 2.5%, 5% and 10%. In the ratio of the inhibitory ability of bacteria at a spesific concentration, the test results showed that S. epidermidis bacteria had different bacterial growth inhibition abilities with S. aureus bacteria. Based on the results obtained, the ethanol extract of wole woe stems has potential as an antimicrobial.
POTENSI ANTIBAKTERI EKSTRAK ETANOL BATANG WOLE WOE ASAL HALMAHERAH TENGAH TERHADAP BAKTERI Pseudomonas aeruginosa dan Bacillus subtilis MENGGUNAKAN METODE DIFUSI AGAR Fitriana Fitriana; Sitti Amirah; Safriani Rahman; Bayu Putra
As-Syifaa Jurnal Farmasi Vol 14, No 2 (2022): AS-SYIFAA JURNAL FARMASI
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jifa.v14i2.904

Abstract

The wole woe plant empirically in the community in Central Halmaherah district is used to treat various diseases, such as breast anticancer, cysts, vaginal discharge, diabetes mellitus, wounds, dysentery, cholesterol, and uric acid, which is thought to have bioactive compounds, that is antibacterial compounds. Therefore, a study was conducted to determine the antibacterial potential of the ethanol extract of wole woe stems against Pseudomonas aeruginosa and Bacillus subtilis bacteria using the agar diffusion method. The results of the screening test showed activity at a concentration of 0.1% and the results of the minimum inhibitory concentration test (MIC) and the minimum killing concentration test (MBC) obtained 1.6% for Pseudomonas aeruginosa bacteria. For Bacillus subtilis bacteria, the MIC value is 0.2% and the MBC value is 3.2%. The statistical test results of the inhibition zone diameter data from the antibacterial activity test against Pseudomonas aeruginosa and Bacillus subtilis bacteria showed that there was a difference in the ability to inhibit bacterial growth between the positive control and the test concentration group of 2.5%, 5% and 10%. The results obtained from the ethanol extract of wole woe stems have potential as antibacterial.