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SKRINING FITOKIMIA EKSTRAK JAMUR KANCING (Agaricus bisporus) Asriani Suhaenah; Siska Nuryanti
Jurnal Fitofarmaka Indonesia Vol 4, No 1 (2017): JURNAL FITOFARMAKA INDONESIA
Publisher : Faculty of Pharmacy, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (815.888 KB) | DOI: 10.33096/jffi.v4i1.228

Abstract

The phytochemical screenings and analysis of chemical compounds in n-hexan extract, ethyl acetat extract and ethanol extract, of Agaricus bisporus with Thin Layer Chromatography (TLC) has been carried out. Extraction process was performed with terraced maceration, identification of bioactive compound with thin layer chromatography. The result of Thin Layer Chromatography test showed that n-hexane extract contains flavonoid, coumarin, therpenoid and steroid. Ethyl acetate extract contains flavonoid, coumarine, therpenoid. Ethanol extract contains flavonoid, alkaloid, polyphenol, coumarin, terpenoid.
POTENSI ANTIBAKTERI EKSTRAK ETANOL JAMUR KANCING (Agaricus bisporus) SEBAGAI ANTIBAKTERI Siska Nuryanti; Fitriana Fitriana
Jurnal Fitofarmaka Indonesia Vol 5, No 1 (2018): JURNAL FITOFARMAKA INDONESIA
Publisher : Faculty of Pharmacy, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (921.175 KB) | DOI: 10.33096/jffi.v5i1.318

Abstract

A research had been done on antibacterial activity of extract and the aim was to determine the antibacterial activity and inhibitory potency. The research was started by screening test using Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Staphylococcus epidermidis, Shigella dysenteriae, Staphylococcus aureus, Streptococcus mutans and Vibrio cholerae. Results showed that the Agaricus bisporus ekstract inhibited Bacillus subtilis, Shigella dysenteriae, Staphylococcus aureus, and Vibrio cholera at concentrations 0,1% and 1%. The results obtained Minimum Inhibitory Concentration (MIC) Agaricus bisporus extract against at concentrations of 0,1%. In the Minimum Bactericidal Concentration (MBC) test Agaricus bisporus extract against at concentrations of 0,8%. Agaricus bisporus extract has antibacterial activity wich was indicated by the inhibitory zone diameter. The biggest inhibitory zone diameter of Agaricus bisporus contained in bacteria Bacillus subtilis on the concentration of 6.4% (13 mm)
ISOLASI FUNGI ENDOFIT KULIT BUAH MERAH (Pandanus conoideus Lam.) YANG BERPOTENSI SEBAGAI ANTIBAKTERI SECARA METODE KLT - BIOAUTOGRAFI Reza Ariyadi Deponda; Fitriana Fitriana; Siska Nuryanti; Herwin Herwin
As-Syifaa Jurnal Farmasi Vol 11, No 2 (2019): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.544 KB) | DOI: 10.33096/jifa.v11i2.583

Abstract

Red fruit (Pandanus conoideus Lam.) belongs to genus Pandanus. Red fruit oil has the activity as an antibacterial. The research aimed to obtain endophytic fungi isolates on the red fruit peel and assay the antibacterial activity by TLC-Bioautography. The research was experimentally conducted including the process of isolation of endophytic fungi, purification, macroscopic examination, screening for antibacterial activity against Staphylococcus aureus and Salmonella typhi, fermentation for 7, 14 and 21 days, separation and antibacterial assay by TLC-Bioautography against Staphylococcus aureus and Salmonella typhi, the isolate fermentate of endophytic fungi for 7 days showed an Rf value of 0.49 against Staphylococcus aureus and an 0.47 against Salmonella typhi. The isolate fermentate of endophytic fungi for 14 days showed an Rf value of 0.58 against Staphylococcus aureus and an 0.69 against Salmonella typhi. The isolate fermentate of endophytic fungi for 21 days showed an Rf value of 0.69 against Staphylococcus aureus and an 0.67 against Salmonella typhi. In conclusion, red fruit peel had endophytic fungi isolate with the potency as an antibacterial.
AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN DAN AMPAS TEH HIJAU (Camellia sinensis L. ) TERHADAP BAKTERI PENYEBAB JERAWAT (Propionibacterium acne DAN Staphylococcus epidermidis ) SECARA DIFUSI AGAR Herwin Herwin; Zulhisda Premeita Sari; Siska Nuryanti
As-Syifaa Jurnal Farmasi Vol 10, No 2 (2018): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.036 KB) | DOI: 10.33096/jifa.v10i2.448

Abstract

Acne is one skin problem commonly known as acne vulgaris, that is a state where the skin pores are clogged. Some of the bakteria causing the growth on acne are Propianibacterium acne and Staphylococcus epidermidis . Green tea (Camellia sinensis L.) is one of the plants that can be useful as antibacterial. This study aimed to obtained the antibacterial activity of ethanol extract leaves and sregs of green tea which has the  potential  to inhibit the growth of acne causing bacteria by the agar diffusion method. The simplisia of green tea leaves and dregs are macerated with ethanol 96% and obtained the weight of ethanol extract of green tea leaves 34.6 grams. In determination of Minimum Inhibitory Concentration (MIC) ethanol extract of the leaves and the dregs, the MIC value obtained is the same as the two test bacteria on the concentration of 0.1%; whereas, KBM value is on the concentration of 0.4%. The result of the antibacterial activity of ethanol extract of green tea leaves at the largest concentration of 8% has an inhibitory zone diameter of 18.11 mm to Propianibacterium acne and 18.05 mm to Staphylococcus epidermidis. For the antibacterial activity test of ethanol extract the green tea dregs at the largest concentration of 8% has the largest average inhibitory zone diameter of 17.45 mm against Propinibacterium acne and 15.68 mm against Staphylococcus epidermidis.
ANALISIS MIKROBIOLOGI BEBERAPA SUSU KEDELAI TANPA MEREK YANG BEREDAR DI KABUPATEN MAROS SULAWESI SELATAN Santri Santri; Siska Nuryanti; Tadjuddin Naid
As-Syifaa Jurnal Farmasi Vol 7, No 2 (2015): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (47.219 KB) | DOI: 10.33096/jifa.v7i2.4

Abstract

Research about the micribiological analyze has been of some soys milk without brand marketed in Maros, South Sulawesi. The research to be compared with the requirements that have been determined by the director-general of the POM. The research conducted by determining the number plate total (ALT) bacteria, Total Plate Number (ALT) moulds as well as the presence of microbial pathogens in some soys milk without brand. The research methods using Standard Plate Count (SPC) to calculate the total amount of bacteria and mold, as wel as the identification method to determine the number of pathogenic bacteria. The results of this study indicating that the sample of C, E and H microbial has fulfilled the requirements of soy milk pathogenic bacteria test of Coliform (-), Salmonella sp (-), Staphylococcus aureus (-) and Bacillus cereus (-). Sample C, E and H has fulfilled the requiremqnts of ALT bacteria and sample C has fulfilled the requiremqnts of ALT moulds. But the E dan H sample have has not fulfilled the requirements.
AKTIVITAS ANTIFUNGI SARI DAUN PEPAYA (Carica papaya L.) TERHADAP Candida albicans Siska Nuryanti
As-Syifaa Jurnal Farmasi Vol 9, No 2 (2017): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/jifa.v9i2.275

Abstract

A research had been done on antifungal activity of papaya leaf ekstract (Carica papaya L.) and the aim was to determine the antifungal activity and inhibitory potency. The research was started by screening  test using Candida albicans. Results showed  that the papayaleaf ekstract inhibited Candida albicans. The research was continued by Minimum Inhibitory Concentration (MIC) test by liquid dilution method in Potato Dekstrosa Broth medium toward, Candida albicans in concentration of MIC 5%, 10%, 15% and 20%. Result showed that papaya leaf ekstract (Carica papaya L.) has antifungal activity wich was indicated by the inhibitory zone diameter in concentration of 10%, 15%, and 20% respectively on Candida albicans.
Asupan Nutrisi Mikro Pada Penderita Tuberkulosis dari Beberapa Ekstrak Tanaman Rachmat Faisal Syamsu; Siska Nuryanti; Ayu Ulfiah Azis
UMI Medical Journal Vol 6 No 1 (2021): Umi Medical Journal
Publisher : Fakultas Kedokteran Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/umj.v6i1.139

Abstract

Menurut Kemenkes 2016 Indonesia memiliki jumlah pasien TB yang ternotifikasi berjumlah 324.539 orang Tuberkulosis (TB) adalah suatu penyakit infeksi menular yang disebabkan bakteri Mycrobacterium tuberculosis, yang dapat menyerang berbagai organ, terutama paru-paru.Orang yang terinfeksi bakteri Mycobacterium tuberculosis akan mengalami gangguan pada sistem kekebalan tubuh. Gangguan tersebut jika bertambah berat akan menyebabkan penurunan status gizi yang ditandai dengan berkurangnya asupan makanan. Status gizi yang rendah dan ketidakmampuan meningkatkan berat badan selama terapi berkaitan erat dengan resiko kematian, terjadinya TB kambuhan, respon terapi yang tidak adekuat, beratnya penyakit TB dan atau adanya penyakit penyerta. Penelitian ini bertujuan untuk mengetahui asupan zat gizi mikro yang dibutuhkan oleh penderita tuberkulosis. Berdasarkan hasil penelitian ini, adapun kesimpulannya adalah beberapa nutrisi mikro untuk penderita tuberkulosis adalah retinol, vitamin D, vitamin E, zink, dan selenium yang banyak terdapat di buah-buahan dan sayur-sayuran, serta senyawa- senyawa aktif yang terdapat dalam bagian-bagian tanaman seperti, alkaloid, flavonoid, terpenoid dan steroid.
IDENTIFICATION OF DERMATOPHYTES BY MULTIPLEX-POLYMERASE CHAIN REACTION, POLYMERASE CHAIN REACTION-RESTRICTION FRAGMENT LENGTH POLYMORPHISM ITS1-ITS4 PRIMERS AND MVAI, AND POLYMERASE CHAIN REACTION (GACA)4 PRIMER Rizalinda Sjahril; Firdaus Hamid; Aan Yulianingsih; Novita Prastiwi; Awaluddin Awaluddin; Siska Nuryanti; Faridha Ilyas; Burhanuddin Bahar
UNEJ e-Proceeding 2016: Proceeding The 1st International Basic Science Conference
Publisher : UPT Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Laboratory identification of skin lesion is important for the correct diagnosis and choice of therapy. Microscopic examination of skin or nail scraping or hair fragments in 10%-KOH provides rapid result but fungal growth in culture is required for identification of species. Unfortunately, culture requires a few days to 2 weeks, and there is variable colony appearance and colour. Rapid and correct diagnosis has been enabled by Polymerase Chain Reaction (PCR), but has not yet been applied for routine diagnosis of patients. Therefore we investigated the ability of culture using Saboraud Dextrose Agar, multiplex-PCR, PCR-RFLP with ITS1-ITS4 primers and MvaI, and PCR with (GACA)4 primer to identify of the etiology agents of 130 patients with tinea who were positive showing hyphae in 10%-KOH preparation. Skin scrapings were collected in Makassar during January-June 2016 and examinations were carried out in the Microbiology Laboratory of Hasanuddin University. Results: Dermatophytosis occurred in 73 (56,1%) males, and 57 (43,8%) females. Scraping was obtained from 78 (60%) skin and 52 (40%) nail lesions. Based on age stratification, 68 (52,3%) were 10-18 years old, 43 (33%) were 19-45 years old, and 19 (14,6%) were >45 years old. While 39 (30%) samples grew in culture, Multiplex-PCR, PCR-RFLP with ITS1-ITS4 primers and MvaI, and PCR with GACA4 primer amplified DNA of 130 (100%), 126 (96,9%), and 106 (81,5 %) samples, respectively. Multiplex-PCR was not able to distinguish between spesies in 99 (76,2%), PCR-RFLP with ITS1-ITS4 primers and MvaI in 29 (22,3%) and PCR with GACA4 primer in 20 (15,4%) samples.
HERBAL YANG BERPOTENSI SEBAGAI ANTI VIRUS PADA COVID-19 Rachmat Faisal Syamsu; Siska Nuryanti; Muh. Farid Jamal
Molucca Medica VOLUME 14, NOMOR 1, APRIL 2021
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (372.5 KB) | DOI: 10.30598/molmed.2021.v14.i1.76

Abstract

SARS CoV-2 merupakan virus Korona penyebab sindrom pernapasan akut berat yang dikenal sebagai Coronavirus Disease 2019. SARS CoV-2 merupakan virus dari keluarga Coronaviridae yang memiliki selubung (envelope). Pandemi virus corona COVID-19 (SARS CoV-2) saat ini telah menghadirkan salah satu kriris kesehatan masyarakat global. Sejak kemunculannya pada Desember 2019 di kota Wuhan, China, virus telah menyebar ke setiap benua. Penelitian ini menggunakan metode review article. Sumber data dalam penelitian ini berasal dari literatur yang diperoleh melalui internet berupa hasil penelitian dari publikasi jurnal Internasional. Hingga saat ini belum ada obat yang pasti disetujui untuk mengobati dan menyembuhkan COVID-19. Karena adanya urgensi situasi, maka dilakukan eksplorasi berbagai alternatif pada obat tradisional untuk mencegah dan mengobati COVID-19. Khasiat dan efektivitas obat herbal telah dikonfirmasi lebih lanjut secara ilmiah melalui beberapa penelitian dari berbagai negara, misalnya, ekstrak air dandelion, ekstrak rumput laut, Ayurveda, Sambiloto, Jeruk, Seledri, tanaman kopiah cina, tanaman artemisin, ginseng india, Aloe vera, kayu manis, Echinacea, Aster tataricus, Rimpang,pakis, bunga telang, jambu biji, kunyit, cengkeh, dan jambu biji untuk mengobati infeksi COVID-19.
KARAKTERISASI ISOLAT BAKTERI PENGHASIL SELULOSA DARI BUAH NAGA MERAH (Hylocereus polyrhizus) Siska Nuryanti; Fitriana Fitriana; A. Rini Pratiwi
As-Syifaa Jurnal Farmasi Vol 13, No 1 (2021): AS-SYIFAA JURNAL FARMASI
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/jifa.v13i1.770

Abstract

Cellulose is a natural polymer widely used for general industrial purposes. The development of science utilizes bacteria from certain fruits to produce biosellulose. Red dragon fruit (Hylocereus polyrhizus) contains a lot of sugar which can be bioconverted to cellulose by cellulose- producing lines. The research aimed to obtain bacteria from red dragon fruit with the potential to produce cellulose, then conduct morphological and biochemical characterization. The research was conducted by the isolation of bacteria from the fruit, purification, and screening tests using Hestrin-Schramm medium. The isolates obtained were characterized morphologically through macroscopic and microscopic observation and the biochemically through catalase test, carbohydrate fermentation, starch and gelatin hydrolysis, motility, and IMViC (Indole, Methyl red, Voges- Proskauer, Citrate). The results showed that 4 bacterial isolates had the potential to produce cellulose. Based on Bergey's Manual of Systematic Bacteriology, 3 bacterial genera were obtained, namely Acetobacter, Gluconacetobacter, and Azotobacter. The results of the characteristics of the isolates obtained at IBBN 2-2 were positive in the catalase test, sucrose and lactose fermentation, and methyl red. IBBN 3-1 was positive in the catalase test, sugar fermentation, starch hydrolysis, methyl red, and citrate. IBBN 3-2 was positive in the sucrose and glucose fermentation test, gelatin hydrolysis and 4-1 IBBN was positive in the sucrose fermentation test and gelatin hydrolysis.