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All Journal Majalah Obat Tradisional As-syifaa Jurnal Farmasi Abdi: Jurnal Pengabdian dan Pemberdayaan Masyarakat Teknologi dan Industri Pertanian Indonesia
Amirullah Amirullah
Universitas Muslim Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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POTENSI LIMBAH AIR TAHU ASAL KOTA MAROS SEBAGAI SUMBER BAKTERI PENGHASIL ENZIM PROTEASE DALAM MELAWAN RADIKAL BEBAS Fitriana Fitriana; Amirullah Amirullah; Nurul Ilmy; Dyah Nur Pratiwi; Andi Adelah Resmilasari; Nabila Adelina HM
As-Syifaa Jurnal Farmasi Vol 8, No 2 (2016): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (123.475 KB) | DOI: 10.33096/jifa.v8i2.205

Abstract

This research aims to determine the protease enzyme-producing bacteria in Limbah Air Tahu  and IC50 fermentat protease enzyme-producing bacteria in Limbah Air Tahu potentially fight free radicals. The methods in the study, which is taking Limbah Air Tahu and sewage treatment, followed by isolation of the bacteria producing the enzyme protease. Bacterial isolates obtained purified, for macroscopic examination. Fermentation bacteria and the production of protease enzymes. Purified protease enzyme obtained. Testing fermentat to fight free radicals with the DPP method and the determination of IC50 values fermentat protease enzyme-producing bacteria. The results of the protease enzyme producing bacteria identification on Biochemical activity test, there were 7 positive bacteria. The results of screening anti-free radical activity against isolates obtained 12 isolates with code FF 05. UMI IBPLT most excellent activity that is obtained of the IC50 fermentat isolates UMI IBPLT FF 05 is equal to 0.129 and dibandingakan denagn IC50 Vitamin C is = 14.81 + 0:10 then isolates FF 05 UMI IBPLT active in fighting free radicals.
Pembuatan Sabun Cair Berbasis Minyak Kelapa Dengan Proses Saponifikasi Untuk Menghambat Pertumbuhan Bakteri Dengan Penambahan Minyak Zaitun (Pure Olive Oil) Untuk Pelembap Kulit Syamsul Bakhri; Amirullah Amirullah; Muhammad Ridha Kasim
Jurnal Teknologi dan Industri Pertanian Indonesia Vol 14, No 1 (2022): Vol. (14) No. 1, April 2022
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (253.811 KB) | DOI: 10.17969/jtipi.v14i1.22567

Abstract

This research was conducted to produce and determine liquid soap products that function as skin moisturizers and can inhibit microbial growth. In the process of making this liquid soap, two formulas are used, namely formula 1 which is made from coconut oil and alkali of KOH with the addition of olive oil and formula 2 which is made from coconut oil and alkali of KOH without the addition of olive oil . The manufacture of liquid soap in this study was carried out experimentally using the hot process method. Organoleptic testing with parameters of foam, skin dryness, and skin moisture was carried out using analytical observational and questionnaire methods involving fifteen correspondents. Testing the inhibition of microbial growth using Staphylococcus aureus bacteria contamination. The results obtained from this research are liquid soap formula 1 and formula 2 have a lot of foam and a very strong microbial growth retardant power, liquid soap formula 1 can moisturize the skin, and liquid soap formula 2 dries the skin. The results of this research, God willing, will be a reference for the author to continue this research to the stage of perfection and it is hoped that the results of this research can also be useful for the ummah and the nation in realizing a healthy life .
Penyuluhan dan Pelatihan Pembuatan Sabun Batang Dan Cair Ekoenzim Pada Guru SMPIT Al-Fikri Makassar Nurmaya Effendi; Hasriwiani Habo Abbas; Amirullah Amirullah; Muhammad Alfinur Putra; Ermy Pertiwi
ABDI: Jurnal Pengabdian dan Pemberdayaan Masyarakat Vol 7 No 1 (2025): Abdi: Jurnal Pengabdian dan Pemberdayaan Masyarakat
Publisher : Labor Jurusan Sosiologi, Fakultas Ilmu Sosial, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/abdi.v7i1.1074

Abstract

Ekoenzim merupakan produk hasil fermentasi sampah organik berupa kulit buah atau sayur dengan gula merah atau molase dan air. Ekoenzim menjadi salah satu cara untuk mengolah sampah organik rumah tangga yang diketahui menjadi salah satu penyumbang sampah hingga melebihi 60% pada tempat pembuangan akhir (TPA). Ekoenzim dapat dimanfaatkan sebagai bahan aktif dalam sabun batang maupun cair yang memiliki nilai ekonomis dan nilai manfaat bagi penggunanya. Tujuan dari kegiatan ini adalah melakukan sosialisasi dan pelatihan pembuatan sabun batang dan sabun cair berbahan aktif ekoenzim bagi guru SMPIT Al-Fikri sebagai bahan praktik pelaksanaan kurikulum merdeka bagi siswa di sekolah mereka pada tema Pola Hidup Berkelanjutan. Evaluasi keberhasilan kegiatan dilakukan melalui pre-test dan post- test untuk mengukur peningkatan pemahaman peserta. Hasil menunjukkan bahwa terjadi peningkatan pengetahuan seluruh peserta dari kurang menjadi cukup memahami (l00%) dan keterampilan semua peserta dari kurang terampil (0%) menjadi kompeten (l00%). Partisipasi aktif peserta dalam diskusi dan praktik pembuatan sabun menunjukkan bahwa kegiatan ini efektif dalam meningkatkan pemahaman dan keterampilan mitra. Melalui kegiatan ini, diharapkan para guru SMPIT Al-Fikri dapat mengajarkan dan menerapkan pengetahuan yang diperoleh dalam program P5 kurikulum merdeka di sekolah dan tentunya dapat mengaplikasikannya dalam kehidupan sehari-hari.
Molecular Analysis and Activities of Bioactive Compounds from Symbiont Bacteria Isolates Polycarpa aurata Herwin Herwin; Gemini Alam; Sartini Sartini; Abdul Rahim; Ayyub Harly Nurung; Amirullah Amirullah; Nasrul Haq
Majalah Obat Tradisional Vol 31, No 1 (2026)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.105930

Abstract

Symbiont bacteria associated with Polycarpa aurata are known to produce secondary metabolites with diverse bioactive properties, including antibacterial, anticancer, antifungal, and antiprotozoal activities. This study evaluated the antibacterial and antibiofilm activities of symbiotic bacterial isolates obtained from the white morphotype of P. aurata collected from Barrang Lompo Island, Makassar City, Indonesia. The agar diffusion method and ELISA reader assay were employed for antibacterial and antibiofilm analyses, respectively. Four pure isolates were obtained and designated AQ2-1, AL2-3, AL2-4, and AL2-5. Molecular identification based on 16S rRNA gene sequencing indicated that isolate AQ2-1 corresponded to Pseudomonas aeruginosa strain AB18. This isolate exhibited a pronounced inhibition zone against Bacillus subtilis, with an optimal incubation period of 68 h for secondary metabolite production. Chemical analysis of the bioactive compounds from isolate AQ2-1 identified adenine riboside and bis(2-ethylhexyl) benzene-1,2-dicarboxylate as the major constituents. Adenine riboside exhibited the highest antibacterial activity, producing an inhibition zone of 12.2 mm against Escherichia coli ATCC 25922, while bis(2-ethylhexyl) benzene-1,2-dicarboxylate produced an inhibition zone of 10.8 mm against Staphylococcus epidermidis ATCC 14990. In the antibiofilm assay, adenine riboside achieved the highest inhibition at 77.09% against Staphylococcus aureus ATCC 25923, whereas bis(2-ethylhexyl) benzene-1,2-dicarboxylate inhibited Streptococcus mutans ATCC 25175 by 73.61%. These findings demonstrate that secondary metabolites produced by the AQ2-1 isolate of symbiont bacteria from P. aurata possess strong antibacterial and antibiofilm activities and may serve as promising candidates for future antimicrobial development.
IDENTIFIKASI DAN PENELUSURAN SENYAWA AKTIF EKSTRAK KULIT JERUK MANIS (Citrus sinensis L. Osbeck) SEBAGAI ANTIBAKTERI TERHADAP BAKTERI PATOGEN Staphylococcus aureus DAN Staphylococcus epidermidis Amirullah Amirullah; Fitriana Fitriana; Mirawati Mirawati; Herwin Herwin; Audia Triani Olii; Nur Sukma Dwi Priyanti; Rasma Maret Halina; Naura Amalia Saliha; Ayu Wulandari
As-Syifaa Jurnal Farmasi Vol 17, No 2 (2025): AS-SYIFAA JURNAL FARMASI
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jifa.v17i2.1396

Abstract

The high prevalence of skin diseases in Indonesia necessitates the exploration of natural therapeutic alternatives, particularly utilizing sweet orange peel (Citrus sinensis L. Osbeck) waste. This study aimed to evaluate the antibacterial efficacy of sweet orange peel extract against skin infection pathogens, specifically Staphylococcus aureus and Staphylococcus epidermidis. Extraction was performed using the Ultrasonic Assisted Extraction (UAE) method. Antibacterial assays included the determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) using the solid dilution method, followed by the identification of active compounds via Thin Layer Chromatography (TLC)-Bioautography. Phytochemical screening revealed the presence of alkaloids, flavonoids, saponins, tannins, and triterpenoids in the extract. The extract demonstrated antibacterial activity with an MIC value of 0.4% and an MBC value of 25.6%. TLC-Bioautography analysis detected inhibition zones corresponding to three Rf values (0.90, 0.32, 0.16) against S. aureus and four Rf values (0.90, 0.67, 0.32, 0.16) against S. epidermidis. The bioactive compounds responsible for this inhibition were identified as alkaloids and flavonoids. In conclusion, the ethanol extract of sweet orange peel exhibits effective antibacterial activity against skin pathogens, mediated by its alkaloid and flavonoid content
Co-Authors Abdul Rahim Andi Adelah Resmilasari Audia Triani Olii Ayu Wulandari Ayyub Harly Nurung Dyah Nur Pratiwi Ermy Pertiwi Fitriana Fitriana Gemini Alam Herwin Herwin Herwin Herwin Mirawati Mirawati Muhammad Alfinur Putra Muhammad Ridha Kasim Nabila Adelina HM Nasrul Haq Naura Amalia Saliha Nur Sukma Dwi Priyanti Nurmaya Effendi Nurul Ilmy Rasma Maret Halina Sartini Sartini Syamsul Bakhri