Hendra Herdian
Bagian Pakan dan Nutrisi Ternak, Balai Pengembangan Proses dan Teknologi Kimia (BPPTK) – LIPI Jln. Jogja-Wonosari Km. 31,5, Gading, Playen, Gunung Kidul, Yogyakarta.

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Effect of Morinda citrifolia leaf as saponin sources on fermentation characteristic, protozoa defaunated, gas and methane production of ruminal fluid in vitro Herdian, Hendra; Istiqomah, L.; Febrisiantosa, A.; Setiabudi, D.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 2 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (85.186 KB) | DOI: 10.14334/jitv.v16i2.639

Abstract

Many studies have reported that the Morinda citrifolia (pace plant) was a useful material for human health. However the exploration of this plant on rumen fermentation is still needed. Therefore, a research was done to study the effect of M. citrifolia leaf on fermentation characteristics of rumen fluid consisted of protozoa defaunated process, VFA composition, NH3 content, rumen microbial protein content, gas and methane production using in vitro techniques. Rumen fluid obtained from two fistulated Ongole crossbreed cattle fed with forage and concentrate feed ration (70 : 30). The fluid was incubated at 39ºC for 48 hours. The treatment on the rumen fluid consisted of control treatment: 100% (200 mg DM) kolonjono forage substrate (Penisetum purpureum) and M. citrifolia treatments: kolonjono forage plus M. citrifolia (equivalent saponin) 3; 6; 9; and 12 mg DM, respectively. The treatment of M. citrifolia leaf addition showed declined patterns in the number of protozoa population (P < 0.05) compared to control. There was no effect of M. citrifolia treatment on VFA composition and NH3 content (P > 0.05). Microbial protein content in rumen fluid increased (P < 0.05) by M. citrifolia treatments compared to control. Effect of M. citrifolia treatments on the gas production was not significantly different (P > 0.05) compared to control, while M. citrifolia treatments reduced the methane gas production of (P < 0.05) compared to control. It was concluded that M. citrifolia leaf has potential as a limiting agent of protozoa population and methane gas production in rumen. Key words: M. citrifolia, Defaunated, Saponin, In Vitro
PROFIL ASAM AMINO EKSTRAK CACING TANAH (LUMBRICUS RUBELLUS) TERENKAPSULASI DENGAN METODE SPRAY DRYING Hayati, Septi Nur; Herdian, Hendra; Damayanti, Ema; Istiqomah, Lusty; Julendra, Hardi
Teknologi Indonesia Vol 34 (2011)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (16.048 KB) | DOI: 10.14203/jti.v34iKhusus.33

Abstract

Antibiotics in poultry were widely used as growth promotor and anti-infective. When antibiotics were used continuously for long periods, it caused microbial resistance. Various attempts were made to replace antibiotics along with the increasing of trend towards demand for healthy, safe and free from harmful residues of livestock products. One of them was application of earthworm which contains high protein and complete amino acids. Earthworms were extracted into water extract form by decoct method. Water extract form had some weaknesses. It was necessary to formulate into a solid dosage form which was more stable, reproducible, and practical. The selected formulation was encapsulation by spray drying method. Quality control of feed additives can be seen from biological value. Biological value correlated positively with amino acids equilibrium or Essential Amino Acid Index (EAAI). The result showed that earthworm extract had both essential and non-essential amino acids higher than earthworm meal and encapsulated earthworm extract. The highest essential amino acid in earthworm meal was isoleucine (3.14%), in earthworm extract was lysine (8.16%), and in encapsulated earthworm extract was leucine (1.71%). The highest non-essential amino acids in earthworm meal and encapsulation of earthworm extract were glutamic acid, respectively 7.67% and 1.87%, while the earthworm extracts was serine (14.52%). The high value of IAAE in earthworm extract showed that the extraction method resulted better amino acid balance (69.87%) than earthworm meal (58.67%). While the value IAAE of encapsulated earthworm extract showed that levels of essential amino acid balance was lower (16.05%) than the earthworm meal (69.87%) and the earthworm extract (58.67%).