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All Journal Jurnal Veteriner BUANA SAINS Jurnal Ilmiah Fillia Cendekia MARSEGU : Jurnal Sains dan Teknologi
Demianus Ferdinand Souhoka
Universitas Pattimura
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Engineering Immunology & microbiology Mathematics Physics Veterinary Other

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Laktosa Mempertahankan Daya Hidup Spermatozoa Kambing Peranakan Etawah yang Dipreservasi dengan Plasma Semen Domba Priangan Demianus Ferdinand Souhoka; Michel Johan Matatula; Wilmientje Marlene Mesang-Nalley; Muhammad Rizal
Jurnal Veteriner Vol 10 No 3 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (113.894 KB)

Abstract

The purpose of this research was to examine the effect of lactose in Tris extender on theviability of Etawah crossbreed goat spermatozoa which is preserved in the plasma using Prianganram seminal plasma at 3–5oC. Semen was collected using artificial vagina once a week. Freshsemen was divided into four tubes, and centrifuged at 3,000 RPM for 30 min. Supernatant wasremoved and replaced which an equal volume with Priangan ram seminal plasma. Semen in firsttube was diluted with Tris extender-20% egg yolk without lactose (control). Semen in second,third, and fourth tubes were diluted with Tris extender-20% egg yolk and 0.3% (0.3 g per 100 mlextender) (L0.3), 0.6% (L0.6), and 1.2% (L1.2) lactose, respectively. Diluted-semen were stored inrefrigerator at 3–5oC. Quality of diluted-semen including percentages of motile spermatozoa (MS),live spermatozoa (LS), and intact plasma membrane (IPM) were evaluated daily during storage at3–5oC for four days. The data were analyzed by usi analysis of variance (ANOVA). Means werecompared significant difference test at 0.05 significant level. Results of this study showed thatmean volume, colour, consistency, pH, mass activity, spermatozoa concentration, MS, LS,spermatozoa abnormal, and IPM of Etawah crossbreed goat fresh semen were 0.68 ml, cream,thick, 7, ++/+++, 4,148.57 million cell/ml, 70%, 83.89%, 7.12%, and 84%, respectively. Addition oflactose in Tris extender could maintain the quality of semen during preservation. At day-5 ofstorage, the percentages of MS, LS, and IPM for treatment L0.6 (40, 55.6, and 53.8%) weresignificantly (P<0.05) higher than treatment L1.2 (36, 50.2, and 51.8%), treatment L0.3 (34, 51.2,and 48.4%), and control (29, 41.4, and 41.8%). In conclusion, addition of 0.6% lactose in Trisextender is the best dose in maintaining the quality of Etawah crossbreed goat spermatozoa, andcould be maintaining percentage of motile spermatozoa 40% for four days after preserved at 3–5oC.
Prevalensi Reaktor Brucellosis Pada Populasi Sapi di Kecamatan Letti, Kabupaten Maluku Barat Daya Astri Dwyanti Tagueha; Demianus Ferdinand Souhoka; Bertha Bira Leklioy
Jurnal Ilmiah Fillia Cendekia Vol 5 No 2 (2020): Jurnal Ilmiah Fillia Cendekia
Publisher : Universitas Islam Kadiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32503/fillia.v5i2.1160

Abstract

Penelitian ini bertujuan untuk mengetahui status infeksi brucellosis pada populasi sapi di Kecamatan Letti, Kabupaten Maluku Barat Daya. Penentuan respoden dilakukan secara purposif sampling dan diambil dari tiga desa yaitu Tomra, Luhulely, dan Batumiau. Variabel yang diamati dalam penelitian ini, antara lain : karateristik peternak, manajemen pemeliharaan, manajemen reproduksi, dan hasil pemeriksaan RBT. Sampel darah diambil dari 48 ekor sapi pada 30 peternakan. Reaktor brucellosis dinyatakan jika Rose Bengal Test (RBT) bernilai positif. Analisis data menggunakan statistik deskriptif, chi-square (x2), dan ratio ganjil (odd ratio) untuk menjelaskan pengaruh variabel dan asosiasinya dengan hasil positif RBT. Hasil penelitian menunjukkan prevalensi reaktor brucellosis di Kecamatan Letti sebesar 21,74% dengan rincian per desa sampel yaitu Luhulely 16,66%, Batumiau 10%, dan Tomra 6,67%. Status kebuntingan berasosiasi positif dengan kejadian brucellosis, sedangkan asosiasi variabel lainnya tidak signifikan secara statistik. Diperlukan penelitian lanjutan dengan sampling deteksi penyakit serta konfirmasi uji serologi dengan complement fixation test (CFT) dan isolasi bakteri.
PREVALENSI MYIASIS PADA KERBAU DI PULAU MOA, KABUPATEN MALUKU BARAT DAYA Jemmy Max Lakuteru; Astri Dwyanti Tagueha; Isak Siwa; Demianus Ferdinand Souhoka; Feronica Parera
BUANA SAINS Vol 22, No 1 (2022)
Publisher : Universitas Tribhuwana Tunggadewi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33366/bs.v22i1.2863

Abstract

The study aimed to determine the prevalence of myiasis in buffalo at Moa island. Data collection was carried out from April to December 2020 using a survey method in 3 villages selected by stratified random sampling. Samples of  98 buffaloes were selected using the detect disease method and taken from four villages. The results showed that the prevalence rate of myiasis in buffalo was 7.14% with details of the prevalence at the village level, namely Tounwawan 9%, Nyama 6%, and Klis 4%. Myiasis was founded in adult buffalo and it was caused by the Chrysomya bezziana. The number of flies per site infestation was 11-23 heads with a duration of 3.09-4.17minutes. The favorable site for oviposition were the neck, back, thighs, and vulva. The predisposing factors that caused the injury were headers, bird pecks, and bush punctures. Maggots had burrowed deep inside causing necrotic cavities, malodorous, and reddish-brown fluid.
LITTERSIZE DAN MORTALITAS TERNAK BABI DARI PARITAS YANG BERBEDA PADA LOKASI INSTALANSI PENGOLAHAN SAMPAH TERPADU DI KOTA AMBON Manurung, Ropanti Uli; Souhoka, Demianus Ferdinand; Labetubun, Jusak
MARSEGU : Jurnal Sains dan Teknologi Vol. 1 No. 8 (2024): MARSEGU : Jurnal Sains dan Teknologi
Publisher : PT. BARRINGTONIA ASIATICA LESTARI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69840/marsegu/1.8.2024.736-747

Abstract

This purpose of this research is to litter size and mortality of pig farming from different parity sows at the integrated waste management installation site in Ambon City. The research method which is conducted is survey method by doing census technique. Variabels that observed are variable treatment in each respons: a) litter size and b) pre-weaning piglet mortality. The results showed that the parity of different parents had no significant effect on litter size. This indicates that the parity factor does not play an important role in influencing the size of the litter born in the population studied. Piglet mortality rates also showed no significant difference among the parity groups. Thus, the parity of sows in this population had no effect on the survival rate of pre-weaning piglets.
PERFORMA REPRODUKSI INDUK TERNAK BABI DI KECAMATAN NUSANIWE KOTA AMBON Pelmelay, Diana M.; Souhoka, Demianus Ferdinand; Labetubun, Jusak
MARSEGU : Jurnal Sains dan Teknologi Vol. 1 No. 9 (2024): MARSEGU : Jurnal Sains dan Teknologi
Publisher : PT. BARRINGTONIA ASIATICA LESTARI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69840/marsegu/1.9.2024.912-920

Abstract

This study aims to determine the reproductive performance of pig farmers in Nusaniwe District, Ambon City. The research was carried out in Nusaniwe District, Ambon City, for 1 month using a survey method with interview techniques. The variables observed in the study were livestock ownership, livestock population, and reproductive performance of the parent including the age of the first mating, the age of the first calving, the length of pregnancy, litter size, calving distance, mortality and signs of fertility. The results showed that the average ownership of pigs based on the age phase of starter, grower, finisher and parent was 9.08 heads (0.9 ST), 6.32 heads (1.26 ST), 1.33 heads (0.53 ST) and 3.33 heads (1.33 ST) respectively. The population structure based on sex is dominated by female cattle 60.75%, and male cattle only 39.24%, while based on the level the respective ages of children were 45.15%, young 23.40%, and adults 23.40%. The reproductive performance of sows showed that the average age of first mating was 9.84±1.89 months, the first calving age was 13.98±1.83 months, the gestation duration was 11628±2.93 days, the litter size was 5.56±2.47 heads, the calving distance was 8.95±1.29 months, and the mortality was 0.33%. The signs of birahi that farmers understand are restless and silent when riding 66.2%, not knowing 33.8%. It was concluded that the reproductive performance of sows in Nusaniwe District was good.
Co-Authors Anak Agung Gede Sugianthara Astri Dwyanti Tagueha Astri Dwyanti Tagueha Bertha Bira Leklioy Feronica Parera Isak Siwa Jemmy Max Lakuteru Jusak Labetubun Manurung, Ropanti Uli Michel Johan Matatula Pelmelay, Diana M. Wilmientje Marlene Mesang Nalley