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All Journal Sainteks Aquasains : Jurnal Ilmu Perikanan dan Sumberdaya Perairan Techno
Heri Maryanto
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Automotive Engineering Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Electrical & Electronics Engineering Energy Industrial & Manufacturing Engineering Mechanical Engineering Medicine & Pharmacology Other

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ISOLASI, KARAKTERISASI, DAN IDENTIFIKASI BAKTERI PADA LELE DUMBO YANG TERSERANG PENYAKIT DI KABUPATEN BANYUMAS Dini Siswani Mulia; Heri Maryanto; Cahyono Purbomartono
Sainteks Vol 8, No 1 (2011): SAINTEKS
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/sainteks.v8i1.299

Abstract

The aim of this research is to isolate, characterize, and identify bacteria on infected African catfish in Banyumas Regency. The researcher used survey method and purposive random sampling technique. Samples were infected African catfish. They were taken from three areas that had potential cultivation for African catfish in Banyumas, namely Rempoah, Kebanggan, and Singosari. In each area, the researcher chose two African catfish cultivating ponds randomly and each pond was taken randomly 3 fishes. Symptoms of the disease in African catfish sample were carefully observed, both externally and internally with surgically. External observation covered wounds and bleeding in some parts of the body, sores or ulcers on the body, protruding eyes, while the internal observation included kidney. Isolation of bacteria carried from the skin (scales), body sores, fin, and kidney. Characterization was done by observing the structure of macroscopic, microscopic, and biochemical tests. The results indicated that 130 isolates of bacteria, and identified to be genus Alcaligenes, Bacillus, Arachnia, Acidomonas, Aeromonas, Amphibacillus, Salmonella, Neisseria, Pseudomonas, Micrococcus, and Clostridium. Keywords: isolation, characterize, identify, bacterium, African catfish, Banyumas regency
POTENTIAL IMMUNOGENICITY OF BACTERIA Aeromonas hydrophila GPL-05 AND GL-02 STRAINS AS A CANDIDATE VACCINES Dini Siswani Mulia; Ani Khusniah; Heri Maryanto
AQUASAINS Vol 4, No 1 (2015)
Publisher : Jurusan Perikanan dan Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.21 KB)

Abstract

Aeromonas hydrophila is very potential to be used in vaccines and disease control MAS (Motile Aeromonas Septicemia) caused by the bacteria. However, strains of A. hydrophila bacteria which have high immunogenicity limited. Not to mention, in the field often decrease the pathogenicity of the bacteria as vaccine material resulting in a decrease in the immunogenicity of a vaccine made. Therefore, it is necessary to inventory and collecting strains of A. hydrophila bacteria potentially good that vaccination continuity can be maintained. This study used an experimental method with a completely randomized design (CRD), with treatments such as P1: A. hydrophila vaccine strain GPL-05; P2: A. hydrophila vaccine strain GL-02; and P3: control (PBS), with five replicates.This study aims to assess the potential immunogenicity A.hydrophila bacterial strains as vaccine candidates. Test fish used is African catfish was about 2 months with a length of 10-15 cm and width 15-20 g. The main parameters are observed antibody titer and cross-reaction test, while supporting parameters are parameters of water quality include water temperature, pH, dissolved oxygen. Antibody titer data was analyzed by Analysis of Variance (ANOVA) and Duncan's Multiple Range Test (DMRT) at the test level of 5%, while the data and the results of cross-reaction of water quality parameters observed descriptively. The results showed that the bacteria A. hydrophila strain GPL-05 and GL-02 has a high immunogenicity, thus recommended a candidate vaccine.
UJI LAPANG PAKAN BERVAKSIN Aeromonas hydrophila PADA LELE DUMBO DI DAERAH CILACAP Dini Siswani Mulia; Sri Wahyuningsih; Heri Maryanto; Cahyono Purbomartono
Techno (Jurnal Fakultas Teknik, Universitas Muhammadiyah Purwokerto) Vol 16, No 2 (2015): Techno Volume 16 No 2 Oktober 2015
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/techno.v16i2.55

Abstract

Masalah utama dalam budidaya lele dumbo adalah penyakit, terutama yang disebabkan oleh bakteri Aeromonas hydrophila. Penyakit tersebut dikenal dengan penyakit MAS (Motile Aeromonas Septicemia). Salah satu penanggulangan penyakit MAS adalah dengan vaksinasi. Masalah lain yang sering muncul adalah tingginya biaya pakan sedangkan harga jual ikan cenderung stabil, sehingga pembudidaya ikan cenderung merugi. Perlu strategi untuk membuat pakan sendiri dari bahan-bahan yang masih memiliki kualitas gizi yang baik tetapi harganya murah bahkan memanfaatkan limbah, bahan tersebut mudah diperoleh dan tersedia setiap saat. Penelitian ini bertujuan untuk menguji secara lapang penggunaan pakan bervaksin pada lele dumbo di daerah Cilacap. Uji lapang dilakukan di Desa Purwodadi, Kecamatan Nusawungu, Kabupaten Cilacap. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 3 perlakuan dan 5 kali ulangan. Perlakuan terdiri dari P1 : pemberian pakan bervaksin selama 10 hari; P2 : pemberian pakan bervaksin selama 15 hari; dan P3 : kontrol (non vaksin). Pakan diberikan sebanyak 5% /bb/ekor/hari. Penelitian menggunakan kolam terpal plastik dengan ukuran panjang x lebar x tinggi : 60 x 60 x 80 cm. Lele dumbo yang digunakan berumur 2 bulan, berukuran panjang 12-15 cm dengan berat 16-25 g. Ikan dipelihara selama 8 minggu. Parameter yang diamati adalah respons imun berupa titer antibodi, pertambahan berat dan panjang, serta sintasan ikan. Parameter pendukung yang diamati adalah parameter kualitas air meliputi suhu air, pH, dan oksigen terlarut. Data dianalisis menggunakan Analysis of Variance (Anova) dan Duncan Multiple Range Test (DMRT) dengan taraf uji 5%. Hasil penelitian menunjukkan bahwa pakan bervaksin dapat meningkatkan produksi titer antibodi (P
Co-Authors Ani Khusniah Cahyono Purbomartono Dini Siswani Mulia