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The In Vitro Antifungal Impact of Secondary Metabolites from Trichoderma sp. on Fusarium sp. Uswatun Hasanah; Arika Purnawati; Herry Nirwanto
Jurnal Agrinika: Jurnal Agroteknologi dan Agribisnis Vol 7 No 2 (2023): SEPTEMBER
Publisher : Kadiri University - Faculty of Agriculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30737/agrinika.v7i2.4511

Abstract

Fusarium sp. is a significant pathogenic fungus responsible for causing wilt in various plants, including chilies, eggplants, and shallots. One approach to address this issue involves utilizing secondary metabolites from the fungus Trichoderma sp. These metabolites were applied at concentrations of 10%, 20%, and 30% alongside a fungicide called M-Dithane 45, following the recommended dosage specified on the packaging. This research aims to evaluate the impact of these secondary metabolites in suppressing the Fusarium sp. pathogen. The study occurred at the Plant Health Laboratory I within the Faculty of Agriculture at Universitas Pembangunan Nasional “Veteran” East Java from October to December 2022. It adopted a single-factor, Completely Randomized Design (CRD), and data analysis was carried out using R-studio software. In vitro analysis was conducted through the dual culture technique on a PDA medium, assessing inhibition percentages and observing the morphology of Fusarium sp. fungi. The results demonstrated that the highest inhibition percentage of Fusarium sp. occurred at a 30% concentration, reaching 26% compared to the control group. Morphological examinations of Fusarium sp. indicated that all treatments affected the growth of abnormal hyphae, including bending, curling, coiling, and lysis.
The Potential of Bacillus spp. in Suppressing Colletotrichum capsici that Causes Antracnose in Red Chili Rani, Sasiska; Endang Triwahyu Prasetyawati; Herry Nirwanto; Sri Wiyatiningsih
International Journal of Applied Biology Vol. 7 No. 1 (2023): International Journal of Applied Biology
Publisher : Hasanuddin University

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Abstract

Red Chili (Capsicum annum L.) is a horticultural plants that is classified as an important commodity and has high economic value. One of the main problems in the production of red chili is anthracnose disease caused by the Colletotrichum capsici pathogen. Bacillus spp. expected to have the potential to inhibit the growth of C. capsici and stimulate the growth of plant. This study aims to determine the potential of Bacillus spp., in suppressing the growth of the C. capsici fungus and stimulating the growth of red chili. This study used a Completely Randomized Design (CRD) with the treatment without Bacillus spp., chemical fungicides, Bacillus sp. (Ba-6), Bacillus sp. (Ba-9), Bacillus sp. (Ba-12), Bacillus sp. (Ba-15), and Bacillus sp. (Ba-17). The treatment was repeated 4 times and the treatment unit contained 5 red chili/polybag. The results showed that the highest suppression of C. capsici in the treatment of Bacillus sp. Ba-15 was 6.25% compared to the negative control. The best bacteria that can stimulate the growth of red chili is Bacillus sp. Ba-9 with an average plant height 20.10 cm and an average number of leaves 10.49