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Efek Refugia terhadap Keberadaan Musuh Alami di Sawah di Desa Kuluran, Kalitengah, Lamongan Noni Rahmadhini; Didik Utomo Pribadi; Arika Purnawati
Plumula : Berkala Ilmiah Agroteknologi Vol 6 No 2 (2018): Plumula : Berkala Ilmiah Agroteknologi
Publisher : UPN VETERAN JAWA TIMUR

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (339.466 KB) | DOI: 10.33005/plumula.v6i2.8

Abstract

Refugia is a microhabitat for natural enemies (predators and parasitoid) to breed that is planted around cultivated plants. The purpose of this study was to determine the effect of the existence of refugia on the abundance of natural enemies in the fields. The method used is the survey method. Observation of natural enemy populations is carried out visually and directly observed. The trap tool used is pitfall and sweep net. The results of the observation showed that 2 families of insects were Branconidae which acted as parasitoid and Collembola which acted as decomposers.
Screening Nematoda Entomopatogen Isolat Jawa Timur Sebagai Agens Pengendali Hayati Serangga Hama Helicoverpa sp. Pada Tanaman Jagung . NUGROHORINI; Arika PURNAWATI; Didik SULISTYANTO; . WAGIYANA
Jurnal Pengendalian Hayati Vol 1 No 2 (2008)
Publisher : Fakultas Pertanian Universitas Jember

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Abstract

Corn is the important food and crop for Indonesian. Recently, production of corn decreased because ofthe pest (Helicoverpa sp.). Until now, the control of Helicoverpa sp. depend on the using of chemicalelement. However, effect of synthetic chemical element usage was the appearance of secondary pest,the death of useful insect and the high accumulation of pesticide residue both in biotic and abioticagroecosystem. The residue of pesticide should effect on human health and environment balance.Furthermore, the control method using biological agents that have pathogenicity to host was done..One of biological agents is entomopathogenic nematodes. Entomopathogenic nematodes can kill it’shost at 24-72 hours, have wide host range such as Lepidoptera, Coleoptera, Diptera and Hymenopteraorder, and have no negatif effect on environment. The aim of the research is to find nematodes asbiological agents that effective against pest of corn (Helicoverpa sp.). The research was done inlaboratory of HPT UPN ”Veteran” Jawa Timur and HPT University of Jember. Methods of theresearch were : 1) Isolating entomopathogenic nematodes from many endemic area of Helicoverpaspp. In East Java; 2) Identificating Entomopatogenic Nematodes; 3) Screening IsolatesEntomopathogenic nematodes from East Java. The result indicated that there was anEntomopathogenic Nematodes Isolate from Tulungagung that has the highest pathogenicity (100%).
Endophytic Bacteria as Biocontrol Agents of Tomato Bacterial Wilt Disease Arika Purnawati
Journal of Tropical Life Science Vol. 4 No. 1 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Ralstonia solanacearum is one important pathogen on tomato because it caused wilt disease that can loss yield until 100%. The object of these experiment are to select of endophytic bacteria that was isolated from healthy tomato stems, root and to investigate of them as biocontrol agents for tomato bacterial wilt disease. There are 10 isolates successfully isolated and two isolates are Ps1 and Ps8 can inhibit of R. solanacearum. The result show the two isolates are Ps1 and Ps8 significantly suppress disease incidence until 8.07-9.19%.
The In Vitro Antifungal Impact of Secondary Metabolites from Trichoderma sp. on Fusarium sp. Uswatun Hasanah; Arika Purnawati; Herry Nirwanto
Jurnal Agrinika: Jurnal Agroteknologi dan Agribisnis Vol 7 No 2 (2023): SEPTEMBER
Publisher : Kadiri University - Faculty of Agriculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30737/agrinika.v7i2.4511

Abstract

Fusarium sp. is a significant pathogenic fungus responsible for causing wilt in various plants, including chilies, eggplants, and shallots. One approach to address this issue involves utilizing secondary metabolites from the fungus Trichoderma sp. These metabolites were applied at concentrations of 10%, 20%, and 30% alongside a fungicide called M-Dithane 45, following the recommended dosage specified on the packaging. This research aims to evaluate the impact of these secondary metabolites in suppressing the Fusarium sp. pathogen. The study occurred at the Plant Health Laboratory I within the Faculty of Agriculture at Universitas Pembangunan Nasional “Veteran” East Java from October to December 2022. It adopted a single-factor, Completely Randomized Design (CRD), and data analysis was carried out using R-studio software. In vitro analysis was conducted through the dual culture technique on a PDA medium, assessing inhibition percentages and observing the morphology of Fusarium sp. fungi. The results demonstrated that the highest inhibition percentage of Fusarium sp. occurred at a 30% concentration, reaching 26% compared to the control group. Morphological examinations of Fusarium sp. indicated that all treatments affected the growth of abnormal hyphae, including bending, curling, coiling, and lysis.