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Pengujian Aktivitas Antibakteri Minyak Atsiri Jahe (Zingiber Officinale Roscoe) Terhadap Bakteri Staphylococcus Aureus dan Escherichia Coli Sufriyana Ali; Maswati Baharuddin; Sappewali Sappewali
Al-Kimia Vol 1 No 2 (2013): December
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (347.992 KB) | DOI: 10.24252/al-kimia.v1i2.1629

Abstract

Testing the antibacterial activity of the Essential Oil of Ginger (Zingiber officinale Roscoe) against bacteria Staphylococcus aureus and Escherichia coli. Purpose of this study is to determine the bacterial growht and activity test active compounds in ginger essential oils that can be used as an antibacterial to inhibit the growth of bacteria. This research uses a method soxhletation with solvent n- hexane to obtain the essential oil of ginger, making the media NA, rejuvenation bacteria, the manufacture of bacterial inoculum and suspension test. Antibacterial activity test using paper disc diffusion method in the form and method of dilution and analysis using GC-MS instrument. Antibacterial activity test result using paper disc method with a concentration of 25%,  50%, 75% and 100% in S. aureus (12, 34; 19, 42; 17, 34; 21, 7) mm and E. coli (10, 56; 13, 76; 16, 5; 23, 6) mm, while the result of the minimum inhibitory concentration (MIC) using ginger essential oil dilution method in S. aureus not found and E. coli at a concentration of 100%. The results of the analysis of essential oil components by GC-MS showed the active compounds in the form of compounds containing hydroxyl groups such as nerol, β- eusdesmol, borneol and compounds containing phenolic groups like zingerone.
Modifikasi Biodiesel Melalui Reaksi Oksidasi Menggunakan Gelombang Ultrasonik Aisyah Aisyah; Sappewali Sappewali; Nurlina Nurlina
Al-Kimia Vol 1 No 2 (2013): December
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.822 KB) | DOI: 10.24252/al-kimia.v1i2.1633

Abstract

Biodiesel is an alternative energy fuel that is sustainable and environmentally safe. However, biodiesel has a higher cloud point than fuel diesel because it contains several saturated fatty acids such as oleic acid, linoleic and linolenic acid so that biodiesel tend to solidify at a temperature higher than the freezing point of diesel. This study aims to modify biodiesel from candlenut oil (Aleurites moluccana) through oxidation reaction by KMnO4 using ultrasonic equipment. The products obtained were analyzed using FTIR and GCMS instrument. FTIR analysis showed that spectral data of the four modified samples which run in 20, 30, 40 and 50 minutes practically similar. The methyl ester products are characterized by typical bands at specific frequencies such as stretch of C = O; C-C; C-H (sp3) and = C-H (sp2). Fragmentation patterns by GCMS showed that the oxidation reaction in 20, 30, 40 and 50 minutes did not undergo bond disconnection. The components produced is methyl palmitate, methyl linoleate, methyl oleate, heksadekanoat acid, 9, 12 and 9,17oktadekadienal oktadekadienoat acid.
Isolasi dan Identifikasi Bakteri Pendegradasi Fenol yang Bersumber Dari Danau Tempe Kabupaten Wajo Sulawesi Selatan Fitriana Fitriana; Maswati Baharuddin; Sappewali Sappewali
Al-Kimia Vol 4 No 2 (2016): December
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (847.388 KB) | DOI: 10.24252/al-kimia.v4i2.1694

Abstract

Water pollution in Tempe Lake was consequence by people activity in around of  Tempe Lake, such as domestic waste and agriculture waste. One of the dangerous pollutions which was resulted from the waste was phenol pollution. Phenol waste reduction efforts on Tempe Lake could be solved by biodegradation process using bacteria. This research aimed to: 1) Get the bacteria which was able to degrade phenol on samples sourced from Tempe Lake, Wajo South Sulawesi and 2) Identify the type of bacteria that can degrade phenol sourced from Lake Tempe, Wajo South Sulawesi. The Metods used in the research was making of media, isolation and purification of bacteria, identification of bacteria and testing the ability of phenol degradation. Bacterial identification tests include staining gram and biochemical tests (TSIA, SIM, MR-VP, urea, citric and sugar fermentation). The degradation test was performed using UV-Vis spectrophotometry with colorimetric method using follin reagent. The results showed that the bacterial identified as the genus Enterobacter spp and Klebsella spp which were gram negative bacteria. Test  of phenol degradation at 48 hour incubation using UV-Vis shows that bacterial isolates C1F was able to degrade phenol 500 ppm to 3,091 ppm, bacterial isolate S1F was able to degrade phenol 500 ppm to 5,1153 ppm and bacterial isolate H2F was able to degrade phenol 500 ppm to 7,7834 ppm.
Sintesis dan Karakterisasi Hidroksiapatit Dari Tulang Ikan Tuna (Thunnus Albacores) Dengan Xrf, Ftir, Dan Xrd St Chadijah; Hardiyanti Hardiyanti; Sappewali Sappewali
Al-Kimia Vol 6 No 2 (2018): December
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/al-kimia.v6i2.5067

Abstract

Hydroxyapatite (Hap) were inorganic compounds with constituent of bones and teeth has the molecular formula Ca5(PO4)3OH. Synthesis of hydroxyapatite can be done with  hydrothermal method  with calcium precursors tuna fish bone calcination process to obtain calcium compounds in the form   of calcium oxide. Calsium wich has been obtained added  phosphate precursor NH4H2PO4 0.547 M. On research conducted long stirring time variation and its effect on rendement and the resulting crystallites products. As for the variation of the long time of stirring used are 1, 2, 3, 4 and 5 hours. The optimum time of formation of hydroxyapatite obtained within 3 hours stirring from which content was 96.4156%. The material was analyzed by FTIR,XRF and XRD. Results showed their characterization by FTIR indicated the presence of OH groups,PO4  and CO3.  Based on the resulting diffraction of  XRF and XRD analysis, crystallite size obtained each of 41,9753  within 2 hours of stirring structure was hexagonal.
Analisis Sifat Fisika Kimia Tanah dan Uji Daya Hambatnya terhadap Bakteri Air Liur AnjingLiar Sjamsiah Sjamsiah; Arifuddin Arifuddin; Mashuri Masri; Sappewali Sappewali; Indah Islamiah; Hardiyanti Hamrullah; Elmika Nesti
Al-Kimia Vol 7 No 1 (2019): JUNE
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/al-kimia.v7i1.7940

Abstract

This study aims to analyze the physical and chemical properties of soil from paddy fields, garden lands and city area also determine the soil inhibitory test of dog saliva bacteria. The results showed that these three soil types have different physical chemical properties and have different inhibitory zones against dog saliva bacteria. The physical chemistry of soil from paddy fields, garden lands and city area (0 cm) are as follows: for color; dark chocolate; grayish brown; and blackish ash, for texture: clay; clay; and sandy clay, for moisture content: 7.14; 6.53; 2.13. for pH close to neutral around 6.52-7.03; Total N is low (0.08-0.23); organic C compounds are classified as moderate; nutrient content of Ca/Mg is classified as moderate (9.55 /1.70 - 14.89/2.22); cation exchange capacity is classified as high (25.32-29.03) in paddy fields and gardens and compounds. The inhibitory zone of the highest dog saliva bacteria is in garden soil (3.7 cm), then paddy soil (2.86 cm) and land in the city area (1.6 cm).  
ISOLASI DAN IDENTIFIKASI KHAMIR PADA LARVA Cossus Cossus PENGHASIL SELULASE Maswati Baharuddin; Nurul Khaerah; Nurul Fadillah; Sappewali Sappewali; Fitria Azis
Teknosains Vol 16 No 3 (2022): September-Desember
Publisher : Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/teknosains.v16i3.29381

Abstract

Larva Cossus cossus adalah serangga ordo Lepidoptera yang hidup di pohon menteng (Baccaurea racemosa) dari Desa Lejja Soppeng. Larva dapat menghasilkan selulase untuk menghidrolisis selulosa menjadi gula sederhana (glukosa) dengan memutuskan ikatan 1,4-glikosidik dalam selulosa. Selulase tekstil digunakan dalam industry detergen, makanan, dan kertas yang dapat diproduksi oleh mikroba seperti selulolitik ragi. Tujuan penelitian ini untuk mengetahui jenis isolat khamir dan bakteri serta aktivitas selulase simbion khamir larva C. cossus. Metode penelitian meliputi isolasi dan pemurnian khamir, identifikasi khamir dan uji aktivitas selulase yang dilakukan setengah kuantitatif (uji yodium dan uji Congo Red) serta uji kuantitatif dengan metode asam dinitrosalisilat (DNS) yang diukur dengan menggunakan spektrofotometer UV-Vis. Hasil penelitian menunjukkan bahwa terdapat 3 isolat bakteri dan 3 isolat jamur yang bersimbiosis dengan larva C. cossus. Isolat dari bakteri Acinotobacter, Pseudomonas dan Bacillus. Isolat dari jamur diidentifikasi secara molekuler diidentifikasi isolat memiliki kesamaan DNA 94% sebagai Paphiopedilum concalor dan kesamaan 90% sebagai strain Cyberlindnera jadinii.
The Influence of Ship Crew Job Involvement on Safety Performance in Enhancing Maritime Safety and Security Systems Joko Purnomo; Irfan Fouzun; Muh. Firah Ramadhan Umar; Muhammad Rifani; Sappewali Sappewali
International Journal of Social Science and Humanity Vol. 1 No. 4 (2024): December : International Journal of Social Science and Humanity
Publisher : Asosiasi Penelitian dan Pengajar Ilmu Sosial Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62951/ijss.v1i4.147

Abstract

Job Involvement is a situation in which an individual feels a psychological attachment to their work and holds a strong belief in completing their tasks to achieve a high level of self-esteem. Safety performance refers to the worker's performance in prioritizing safety in the workplace by adhering to the applicable health and safety regulations. The purpose of this study is to determine the effect of Job Involvement on Safety Performance among ship crew members in improving the safety and security system of maritime operations. The research was conducted at PT. Tonasa Lines, Biring Kassi, Pangkep Regency, South Sulawesi Province. The sample in this study consisted of 100 participants. The sampling techniques used in this research were both probability sampling and non-probability sampling. The method used in this research was quantitative, using the Job Involvement and Safety Performance scales. The data analysis was performed using the Product Moment correlation technique with the assistance of the SPSS 28.0 for Windows program. The results showed a significant positive relationship between the Job Involvement variable and Safety Performance in improving the maritime safety and security system. The significance level was below or equal to 0.040 (p ˃ 0.01), so Ha is accepted and Ho is rejected with a 5% margin of error probability.