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All Journal Biogenesis: Jurnal Ilmiah Biologi
Niken Satuti Nur Handayani
Fakultas Biologi UGM
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Karakter Morfologis dan Molekular Macrobrachium spp. dari Sungai Opak Daerah Istimewa Yogyakarta Trijoko T; Niken Satuti Nur Handayani; Anita Widianawati; Rury Eprilurahman
Biogenesis: Jurnal Ilmiah Biologi Vol 3 No 1 (2015)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v3i1.559

Abstract

Several rivers flow across Daerah Istimewa Yogyakarta (DIY includes Opak River which it’s upstream located on Merapi Volcano area. The river has a lot of biological resources, includes prawns. Freshwater prawns (Macrobrachium spp.) has important role in maintaining the balance of freshwater ecosystems. The purpose of this research was to determine species diversity of Macrobrachium spp. and to understand the morphological and molecular characters of them. Phenetic similarity was analyzed using 74 morphological characters, ISSR1 and ISSR3 primers. The results were analyzed using a program MVSP 3.1 (Multi- Variate Statistical Package) to obtain a dendogram that showed both intra-and interspecies similarity of Macrobrachium spp. Six species of Genus Macrobrachium were identified, they are Macrobrachium pilimanus, M. sintangense, M. lanchesteri, M. cowlesi, M. latidactylus, and M. equidens. Both morphological and molecular analyses show two major clusters with the similarity of 55.6% for morphology and 21.52% for molecular. Based on morphological and molecular characters analysis, we concluded that phenetic similarity of Macrobrachium spp. in Opak River was affected by the morphological structure of the second pereiopod, habitat type, size and number of eggs, as well as the type of larval development.Keywords: characters, Macrobrachium, molecular, morphology, Opak River
Karakterisasi Kromosom Stroberi (Fragaria vesca L. subsp. californica Cham. & Schltdl. cv. Californica) Hasil Poliploidisasi Rosyidatul Khoiroh; Ganies Riza Aristya; Sutikno S; Niken Satuti Nur Handayani
Biogenesis: Jurnal Ilmiah Biologi Vol 3 No 2 (2015)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v3i2.932

Abstract

Fruit consumption such as strawberries always increase in Indonesia and reaching about 210 tons in 2011. Strawberry (Fragaria vesca L. subsp. californica Cham. and Schltdl. cv. Californica) has been developed in Indonesia, especially in Banyuroto, Sawangan, the district of Magelang, Central Java. In order to improve the fruit productivity (quality and quantity), geneticcharacterization including chromosomes character of this fruit are needed to be investigate. Therefore, the research was conducted to determine the chromosomes number, chromosomes size chromosomes shape and karyotype of control and strawberries treated by colchicine as mutagenic agent. The method was squash and flow cytometry method. Whereas the cell suspension samplewere observed used flow cytometer instrument. The result of research shown that the chromosome number of strawberry Californica in normal and treatment strawberries was 2n=14. Thechromosomes shape of normal strawberry was 10 metacentric and 4 submetacentric. Whereas the chromosomes shape of treatment strawberries used colchicine was 10 metacentric and 4submetacentric. Flow cytometric analysis shown that control strawberries and treated strawberries has diploid ploidy level.Keywords: chromosomes, colchicine, flow cytometry, polyploidization, strawberry Californica
Co-Authors Anita Widianawati Ganies Riza Aristya Rosyidatul Khoiroh Rury Eprilurahman Sutikno S Trijoko T