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Karakter Fenotipik Tanaman Stroberi Festival (Fragaria x ananassa D.) Hasil Induksi Kolkisin Pada Konsentrasi 0,05% dan 0,01% Riza Aristya, Ganies; Setiadi Daryono, Budi
Biogenesis Vol 2, No 2 (2014)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

Strawberries were a lot of holticulture commodities consumed by the people of Indonesia. Strawberry consumption continues to increase over time in Indonesia but not matched by production. This it because the strawberry is native to subtropical climate so that production in Indonesia limited in the plateau regional still affected by the tropical climate. Therefore, it is necessary to increase the production of both quality and quantity of strawberries, one with plant breeding through the induction of colchicine. The objective of this research were to determine the differences in phenotypic characters of strawberry plants with the control and induction results to determine the length of time and the induction colchicine 0,05% and 0,01% on the Festival cultivar of strawberry most effective. Research were done by inducing with 0,05% and 0,01% colchicine in leaves, roots 24 hours and 36 hour, the root and leaves of 24 and 36 hour. The observed for 6 months long leaf growth, leaf width,number of leaves, stem circumference, plant height, bloom vast flower and volumes of the fruit. The results indicate that the induction of the leaf growth at the optimal parameter leaf lenghth, leaf width and number of leaves. While 24-hour induction of root growth at optimum height and volume of fruitplants, then root induction at 36 hour to flowers bloom. Induction in root and leaves 36 hour optimum growth on trunck circumference. From this study it could be concluded that there are differences in the character of the strawberry crop cultivar Festival phenotype induction results colchicine 0,05% and 0,01% with control. Induction for 36 hours and induction on leaves was most effective for induction of strawberry cultivar Festival.Keywords: character phenotype, colchicine, festival
Development of Random Amplified Polymorphism DNA Markers Linked to Powdery Mildew Resistance Gene in Melon Daryono, Budi Setiadi; Aristya, Ganies Riza; Kasiamdari, Rina Sri
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

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Abstract

A random amplified polymorphic DNA (RAPD) marker linked to powdery mildew resistance gene (Pm-I) in melon PI 371795 was reported. However, the RAPD marker has problem in scoring. To detect powdery mildew resistance gene (Pm-I) in melon accurately, the RAPD marker was cloned and sequenced to design sequence characterized amplified region (SCAR) markers. SCAPMAR5 marker derived from pUBC411 primer yielded a single DNA band at 1061 bp. Segregation of SCAPMAR5 marker in bulk of F2 plants demonstrated that the marker was co-segregated with RAPD marker from which the SCAR marker was originated. Moreover, results of SCAR analysis in diverse melons showed SCAPMAR5 primers obtained a single 1061 bp linked to Pm-I in resistant melon PI 371795 and PMAR5. On the other hand, SCAPMAR5 failed to detect Pm-I in susceptible melons. Results of this study revealed that SCAR analysis not only confirmed melons that had been clearly scored for resistance to Pm-I evaluated by RAPD markers, but also clarified the ambiguous resistance results obtained by the RAPD markers.   Key words: Cucumis melo L., Pm-I, RAPD, SCAPMAR5
Phylogenetic Relationships of Nine Cultivars of Strawberries (Fragaria spp.) Based on Anatomical and Morphological Characters Kasiamdari, Rina Sri; Aristya, Ganies Riza; Inayati, Evi
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 5, No 2 (2017)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2017.072.116-126

Abstract

Strawberry cultivation in Indonesia is centred at Citrus and Subtropical Fruit Research Institute, which collects different cultivars of Fragaria spp. Information on classification based on phenotypic characters of Fragaria spp. has not been studied as a whole. The purpose of the present study was to identify and determine phylogenic relationships of nine cultivars of strawberries based on the anatomical and morphological characters. The present study identified morphologically nine strawberry cultivars by referring to the IPGRI (1986) and the UPOV (2012) and Hofer et al. (2012). Anatomical preparations of roots, stems and leaves were made by the embedding method. Phylogenetic relationships were determined using MVSP software with UPGMA algorithm through the Gower General Similarity Coefficient method. Subsequently, the principal components were analyzed using the Euclidian Biplots algorithm depicted in a Scatter plot. Results showed that there were 37 anatomical and morphological characters that play a role in the characterization of nine cultivars (25 synthetic characters and 12 diagnostics characters) where the shape of the fruit and type of attachment of the flowers were the main characters distinguishing among the nine cultivars studied. The phylogenetic relationship produced two clusters with 79% similarity index. The closely related cultivars were Festival and Rosa Linda with a similarity index of 94%, and Earlibrite and Aerut with a similarity index of 86%
KARAKTER GEN CMBG1 MELON (CUCUMIS MELO) PADA PENGARUH CEKAMAN TANAH KARST Aristya, Ganies Riza; Daryono, Budi Setiadi; Rachmawati, Yuanita
Sains & Matematika Vol 3, No 1 (2014): Oktober, Sains & Matematika
Publisher : Sains & Matematika

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Abstract

Lahan kritis berkapur, karst, memberikan cekaman abiotik pada tanaman karena hambatan hidrasi dan nutrisi. Asam absisat (ABA) adalah hormon yang diekspresikan tumbuhan pada kondisi cekaman abiotik. CmBG1 merupakan salah satu gen peregulasi hormon ABA pada melon yang akan terakumulasi saat tumbuhan mengalami cekaman. Tujuan penelitian ini adalah mendeskripsikan pengaruh lahan kritis karst terhadap ekspresi gen CmBG1 secara kualitatif dan kuantitatif melon hasil turunan kultivar TACAPA, yaitu kultivar PT dan AT yang ditanam pada medium tanah karst dari wilayah Agroekosistem II dan III Yogyakarta (Gunungsewu, Dlingo, maupun Sentolo). cDNA library diperoleh dari reverse transcription isolat RNA. cDNA diamplifi kasi dengan primer spesifi k, kemudian dispektrofotometri pada ?260 nm untuk mengetahui konsentrasi gen CmBG1. Ekspresi gen dianalisis dengan real time PCR dengan gen referensi Cm-actin. Uji kualitatif dilakukan dengan elektroforesis gel agarosa 1,5%. Hasil penelitian menunjukkan gen CmBG1 terdeteksi dengan ukuran ±1258 bp pada kultivar PT dan AT. Konsentrasi gen CmBG1 melalui spektrofotometri menunjukkan semua kultivar yang ditanam pada media kontrol memiliki konsentrasi yang lebih rendah bila dibandingkan media tanam dengan perlakuan lahan kritis baik Gunungsewu, Dlingo, maupun Sentolo. Hasil ini sama dengan uji ekspresi gen CmBG1 menggunakan analisis kuantitatif real time PCR. Karsts critical land gives abiotic stresses in plants because of hydration and nutrition disturbances. Abscisic acid (ABA) is a hormone that expressed in the plant in abiotic stress conditions. CmBG1 is one of the regulatory genes encoding hormone ABA in melon plants which is accumulated in stress condition. The purpose of this study was to describe the infl uence of karsts critical land on the genes expression of CmBG1 melon cultivars PT and AT qualitatively and quantitatively. The plants were grown in medium karst land of Agroecosystems II and III of Yogyakarta (Gunungsewu, Dlingo, and Sentolo). Total RNA was extracted from leaf tissue then Reversed Transcriptase (RT-PCR) to collect cDNA library. cDNA was amplifi ed using specifi c primer. Spectrophotometry was conducted in ?260 nm and electrophoresis run in 1.5% agarose gel. Control band and reference gene chosen in Real Time PCR was Cm-Actin. CmBGI band (± 1258 bp) was showed both on PT and AT. Cm-actin was showed band of DNA as ± 445 bp. CmBGI gene concentration in critical land medium treatment which is given greater stress on melons are higher than normal condition. This suggests that the CmBGI gene is expressed more in cultivar PT and AT melons when they are grown under stress condition. This result show similarly when using real time PCR.
DETECTION AND IDENTIFICATION OF POLYMORPHISM IN MUTANT STRAWBERRY (FRAGARIA SPP.) PLANTS BASED ON CLEAVED AMPLIFIED POLYMORPHIC SEQUENCES MOLECULAR MARKERS Aristya, Ganies Riza; Ayundai, Melin; Putri, Fauzana; Widiastuti, Ani; Kasiamdari, Rina Sri
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 8, No 1 (2020)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2020.109.15-20

Abstract

Strawberry (Fragaria spp.) has a high economic value and various benefits, but the production of strawberry plants in Indonesia is still low in terms of both quantity and quality. Strawberry plant breeding can be done in various techniques, one of which is polyploidization. Polyploidization by an induction of colchicine at various concentrations in strawberry crops cv. California and Festival resulted in superior phenotype characteristics. To prove the existence of a change in ploidy in strawberry, then research at the molecular level needs to be done. The purposes of this study were to find out changes in ploidy of strawberry plants using CAPS molecular markers and to detect the polymorphism in strawberry plants quickly. The samples used were young leaves. Main procedure was the cutting of the amplified DNA using restriction enzymes of TaqI and HaeIII. The results showed that CAPS molecular markers were capable of detecting polymorphism quickly and efficiently in strawberry plants. Specific bands among strawberry plants having undergone polyploidization and those not having undergone polyploidization can be seen on the differences in monomorphic or polymorphic bands between the control plants and treated plants. 
Karakter Gen CmBG1 Melon (Cucumis melo) pada Pengaruh Cekaman Tanah Karst Aristya, Ganies Riza; Daryono, Budi Setiadi; Rachmawati, Yuanita
Sains & Matematika Vol 3, No 1 (2014): Oktober, Sains & Matematika
Publisher : Sains & Matematika

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Lahan kritis berkapur, karst, memberikan cekaman abiotik pada tanaman karena hambatan hidrasi dan nutrisi. Asam absisat (ABA) adalah hormon yang diekspresikan tumbuhan pada kondisi cekaman abiotik. CmBG1 merupakan salah satu gen peregulasi hormon ABA pada melon yang akan terakumulasi saat tumbuhan mengalami cekaman. Tujuan penelitian ini adalah mendeskripsikan pengaruh lahan kritis karst terhadap ekspresi gen CmBG1 secara kualitatif dan kuantitatif melon hasil turunan kultivar TACAPA, yaitu kultivar PT dan AT yang ditanam pada medium tanah karst dari wilayah Agroekosistem II dan III Yogyakarta (Gunungsewu, Dlingo, maupun Sentolo). cDNA library diperoleh dari reverse transcription isolat RNA. cDNA diamplifi kasi dengan primer spesifi k, kemudian dispektrofotometri pada λ260 nm untuk mengetahui konsentrasi gen CmBG1. Ekspresi gen dianalisis dengan real time PCR dengan gen referensi Cm-actin. Uji kualitatif dilakukan dengan elektroforesis gel agarosa 1,5%. Hasil penelitian menunjukkan gen CmBG1 terdeteksi dengan ukuran ±1258 bp pada kultivar PT dan AT. Konsentrasi gen CmBG1 melalui spektrofotometri menunjukkan semua kultivar yang ditanam pada media kontrol memiliki konsentrasi yang lebih rendah bila dibandingkan media tanam dengan perlakuan lahan kritis baik Gunungsewu, Dlingo, maupun Sentolo. Hasil ini sama dengan uji ekspresi gen CmBG1 menggunakan analisis kuantitatif real time PCR. Karsts critical land gives abiotic stresses in plants because of hydration and nutrition disturbances. Abscisic acid (ABA) is a hormone that expressed in the plant in abiotic stress conditions. CmBG1 is one of the regulatory genes encoding hormone ABA in melon plants which is accumulated in stress condition. The purpose of this study was to describe the infl uence of karsts critical land on the genes expression of CmBG1 melon cultivars PT and AT qualitatively and quantitatively. The plants were grown in medium karst land of Agroecosystems II and III of Yogyakarta (Gunungsewu, Dlingo, and Sentolo). Total RNA was extracted from leaf tissue then Reversed Transcriptase (RT-PCR) to collect cDNA library. cDNA was amplifi ed using specifi c primer. Spectrophotometry was conducted in λ260 nm and electrophoresis run in 1.5% agarose gel. Control band and reference gene chosen in Real Time PCR was Cm-Actin. CmBGI band (± 1258 bp) was showed both on PT and AT. Cm-actin was showed band of DNA as ± 445 bp. CmBGI gene concentration in critical land medium treatment which is given greater stress on melons are higher than normal condition. This suggests that the CmBGI gene is expressed more in cultivar PT and AT melons when they are grown under stress condition. This result show similarly when using real time PCR.
Detection and Identification of Polymorphism in Mutant Strawberry (Fragaria spp.) Plants Based on Cleaved Amplified Polymorphic Sequences Molecular Markers Aristya, Ganies Riza; Ayundai, Melin; Putri, Fauzana; Widiastuti, Ani; Kasiamdari, Rina Sri
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 8, No 1 (2020)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2020.109.15-20

Abstract

Strawberry (Fragaria spp.) has a high economic value and various benefits, but the production of strawberry plants in Indonesia is still low in terms of both quantity and quality. Strawberry plant breeding can be done in various techniques, one of which is polyploidization. Polyploidization by an induction of colchicine at various concentrations in strawberry crops cv. California and Festival resulted in superior phenotype characteristics. To prove the existence of a change in ploidy in strawberry, then research at the molecular level needs to be done. The purposes of this study were to find out changes in ploidy of strawberry plants using CAPS molecular markers and to detect the polymorphism in strawberry plants quickly. The samples used were young leaves. Main procedure was the cutting of the amplified DNA using restriction enzymes of TaqI and HaeIII. The results showed that CAPS molecular markers were capable of detecting polymorphism quickly and efficiently in strawberry plants. Specific bands among strawberry plants having undergone polyploidization and those not having undergone polyploidization can be seen on the differences in monomorphic or polymorphic bands between the control plants and treated plants. 
Karakter Fenotipik Tanaman Stroberi Festival (Fragaria x ananassa D.) Hasil Induksi Kolkisin Pada Konsentrasi 0,05% dan 0,01% Ganies Riza Aristya; Budi Setiadi Daryono
Biogenesis: Jurnal Ilmiah Biologi Vol 2 No 2 (2014)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v2i2.470

Abstract

Strawberries were a lot of holticulture commodities consumed by the people of Indonesia. Strawberry consumption continues to increase over time in Indonesia but not matched by production. This it because the strawberry is native to subtropical climate so that production in Indonesia limited in the plateau regional still affected by the tropical climate. Therefore, it is necessary to increase the production of both quality and quantity of strawberries, one with plant breeding through the induction of colchicine. The objective of this research were to determine the differences in phenotypic characters of strawberry plants with the control and induction results to determine the length of time and the induction colchicine 0,05% and 0,01% on the Festival cultivar of strawberry most effective. Research were done by inducing with 0,05% and 0,01% colchicine in leaves, roots 24 hours and 36 hour, the root and leaves of 24 and 36 hour. The observed for 6 months long leaf growth, leaf width,number of leaves, stem circumference, plant height, bloom vast flower and volumes of the fruit. The results indicate that the induction of the leaf growth at the optimal parameter leaf lenghth, leaf width and number of leaves. While 24-hour induction of root growth at optimum height and volume of fruitplants, then root induction at 36 hour to flowers bloom. Induction in root and leaves 36 hour optimum growth on trunck circumference. From this study it could be concluded that there are differences in the character of the strawberry crop cultivar Festival phenotype induction results colchicine 0,05% and 0,01% with control. Induction for 36 hours and induction on leaves was most effective for induction of strawberry cultivar Festival.Keywords: character phenotype, colchicine, festival
Karakterisasi Morfologi dan Anatomi Stroberi (Fragaria x ananassa D. cv. Festival) Hasil Induksi Kolkisin Ana Susianti; Ganies Riza Aristya; Sutikno S; Rina Sri Kasiamdari
Biogenesis: Jurnal Ilmiah Biologi Vol 3 No 2 (2015)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v3i2.929

Abstract

Strawberry is a plant cultivated in subtropical countries, while Indonesia’s tropical climate. This is not accordance with the conditional of the natural growth of strawberries. Strawberries cultivar Festival is one of holticulture products in society. The high rates of consumption of strawberries are not equal with production and quality of strawberries. The quality of strawberries were determined by fruit size and uniformity of fruit. Effort that can be used to improve the qualityof strawberry production is polyploiditation with mutagenic substance, example colchicine. Result of polyploidy in morphological character are bigger. Technique of polyploidy with colchicine will be success if the number and structure of chromosom in cell nucleus are changes. Chromosome number changes will be change to anatomy, morphology and physiology plants. The aims of this research were to know the differences of anatomical and morphological characters of strawberry cultivar Festival after colchicine induction and find out the influence of the colchicine concentration differences in morphological and anatomical character of strawberry cultivars Festival. The research was have two main stages. The first were observation and scoring of 25 morphological characters of strawberries after colchicine induction with 0%; 0,01% and 0,05%. The second, preparations with paraffin method on the root, steam, leaves and fruit of the strawberry plant after colchicine induction. The observation of anatomical characters are measurements number cell was from of the root, steam, leaves and fruits. The results showed that induction of colchicine in strawberry cultivars festival showed different morphological characters on the density of the leaves, number of stems, fruit ripening time, fruit size, fruit sweetness of the outer color of the fruit, fruit acidity compared to control plants. Anatomical characters of cell size increases the cortex and pith of stems, leaves, cell mesofil cells of the cortex and pith of fruit. Colchicine induction of 0.05% was more effective in increasing the size of the fruit while the induction of colchicine of 0.01% was more effective at increasing the size of vegetative organs of strawberry cultivars Festival plants.Keywords: anatomy, colchicine, Festival Cultivars, morphology, strawberry
Karakterisasi Kromosom Stroberi (Fragaria vesca L. subsp. californica Cham. & Schltdl. cv. Californica) Hasil Poliploidisasi Rosyidatul Khoiroh; Ganies Riza Aristya; Sutikno S; Niken Satuti Nur Handayani
Biogenesis: Jurnal Ilmiah Biologi Vol 3 No 2 (2015)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v3i2.932

Abstract

Fruit consumption such as strawberries always increase in Indonesia and reaching about 210 tons in 2011. Strawberry (Fragaria vesca L. subsp. californica Cham. and Schltdl. cv. Californica) has been developed in Indonesia, especially in Banyuroto, Sawangan, the district of Magelang, Central Java. In order to improve the fruit productivity (quality and quantity), geneticcharacterization including chromosomes character of this fruit are needed to be investigate. Therefore, the research was conducted to determine the chromosomes number, chromosomes size chromosomes shape and karyotype of control and strawberries treated by colchicine as mutagenic agent. The method was squash and flow cytometry method. Whereas the cell suspension samplewere observed used flow cytometer instrument. The result of research shown that the chromosome number of strawberry Californica in normal and treatment strawberries was 2n=14. Thechromosomes shape of normal strawberry was 10 metacentric and 4 submetacentric. Whereas the chromosomes shape of treatment strawberries used colchicine was 10 metacentric and 4submetacentric. Flow cytometric analysis shown that control strawberries and treated strawberries has diploid ploidy level.Keywords: chromosomes, colchicine, flow cytometry, polyploidization, strawberry Californica
Co-Authors Abdul Razaq Chasani, Abdul Razaq Abdul, Chasani Razaq Abiyyi, Muhammad Hawi Qabus Afifah, Nasywa Kamila Agus Setiawan Akbar, Ananda Malik Almira Syafataya Amadeus, Sovann Ana Susianti Ani Widiastuti, Ani Aries Bagus Sasongko Aurantika, Rindu Ayundai, Melin Ayundai, Melin Azzahira, Faiha Azka Bartolomius Renaldy Bening Larasati Budi S Daryono BUDI SETIADI DARYONO Chalvia Zuyyina Christophorus Ardhito Haryo Dwinanda Dea Febiansi Dewi, Umi Rosita Dian Astriana Dimas, Abimanyu Dita Yulistiani Dyanita, Anissa Cahyadevi Elva Maulida Khozana Fadhlurrohman, Galih Dzaky Fajar Sofyantoro Fauzana Putri Febri Yuda Kurniawan Galang Riswi Dyatama Hasanah, Muslifah Hasibuan, Aldy Riau Wansyah Hevi Wihadmadyatami Hidayat, Madyan Akmal Himawan Masyhuri Inayati, Evi Indra Lesmana Ishana, Aurelia Araya Karmilah, Karmilah Kusnah Dian Prasiwi Latifah, Vida Rahma Lisna Hidayati Mif Ardianata Pratama Putra Muhammad Mufid Al-Dayyan Muhammada, Khusnus Syifa Muliawati, Adina Nainggolan, Ananto Puradi Nasywa Kamila Afifah Niken Satuti Nur Handayani Niken Satuti Nur Handayani Nugroho, Rifat Rizki Adi Nugroho Nurcahyati, Vivi Indah Nurhasanah, Anggun Diyan Pratiwi, Arini Dian Putri, Fauzana Putri, Fauzana Ramadhani, Gilang Afif Setya Rezika Alyza Rifa Ayuningsih Rifat, Nugroho Rizki Adi Rina Sri Kasiamdari Rispaini Nur Aziza Risydah, Fiqiha Rosyidatul Khoiroh Safitri, Della Thasya Liona Salsabila, Sophia Sholihatun Nissa Sigit Dwi Maryanto Sofyantoro, Fajar Sritanjung, Opi Sutikno S Syafei, Dio Rachmaputra Tasyarofa, Alya Teguh Budipitojo Triska Ayu Nurwijayanti Tuty Arisuryanti, Tuty Ulfa, Siti Zakia Uswatun Mujahidah Utami, Mia Widiastuti, Ani Widyasari, Adristi Shafa Wulandari, Mutiara Tri Yuanita Rachmawati Yuda, Khalid Abdillah Abdurahman Yusuf, Adib Fakhruddin