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All Journal Biogenesis: Jurnal Ilmiah Biologi Journal of Biodiversity and Biotechnology
Edi Purwanto
Sebelas Maret University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Profile of microbial community of organic and conventional rice field using metagenomic analysis Arum Asri Trisnastuti; Edi Purwanto; Ari Susilowati
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.14228

Abstract

Organic farming can increase the diversity of soil bacterial. This research aimed to compare the profile of microbial community of organic and conventional rice fields in early (0 Day After Planting/DAP), mid (15 DAP), and late (45 DAP) fertilizer application period. The total DNA genome from the soil sample was extracted then analyzed metagenomically using Next Generation Sequencing (NGS). There was nine genus of bacteria found in high relative abundance, 95.28%, while 4.72% included in Domain of Archaea (genus Methanosaeta). Phylum of Firmicutes (genus Clostridium has 24.50% relative abundance, Bacillus 11.90%, Lactobacillus 9.69%); Proteobacteria (genus Defluviicoccus 12.10%, Buchnera 18.46%, Rosenbergiella 2.46%); and Actinobacteria (genus Nocardioides 12.21%, and Streptomyces 3.96%). Meanwhile, the average plant height of organic rice fields was shorter than conventional rice fields got directly measured coincided with soil sampled. Based on alpha and beta diversity analysis, the highest community diversity and abundance were found in organic rice field soil samples taken at 45 DAP, i.e., at the end of the fertilizer application period. However, in both organic and conventional rice field soils, there was almost no significant difference in the bacterial community, so it impacts that organic and conventional systems do not make a real difference in the total N, P available, CEC, and pH values. It makes a significant difference in organic C and organic matters.
The Effect of Naphtaleine Acetic Acid ND Benzyl Amino Purine on the Subculture of Kepok Unti Sayang Banana Samanhudi Samanhudi; Andriyana Setyawati; Edi Purwanto; Muji Rahayu; Eviani Eviani; Muhammad Aji Cahyadi
Journal of Biodiversity and Biotechnology Vol 5, No 1 (2025)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v5i1.108513

Abstract

The Kepok Unti Sayang variety is a superior type of banana that is resistant to fusarium wilt. The high demand for Kepok Unti Sayang needs to be balanced with good seed production. One of the methods used for multiplying superior seeds of Kepok Unti Sayang is through tissue culture. This research aims to obtain the optimal concentration of Naphtaleine Acetic Acid (NAA) in the subculture media for the growth of root numbers in the Kepok Unti Sayang Banana. The second objective is to determine the optimal concentration of Benzyl Amino Purine (BAP) in the subculture medium for shoot number growth in Kepok Unti Sayang bananas. Another goal is to identify the best treatment combination that can produce the highest number of shoots in Kepok Unti Sayang bananas. The research was conducted at the Laboratory of Plant Physiology and Biotechnology at Sebelas Maret University from February to July 2025. The study was conducted with 4 levels of NAA treatment: 0; 0.5; 1; and 1.5 ppm. The BAP treatment was administered at 4 levels: 0; 1; 2; and 3 ppm. The variables observed in this study were the time of shoot emergence, number of shoots, time of root emergence, root length, and final height of the planlet. Data from each variable were then analyzed using analysis of variance (ANOVA) with an F-test at a significance level of 95%. Significantly different data were then followed by Duncan's Multiple Range Test (DMRT) at a significance level of 95%. The significant combination of NAA and BAP on the observed variables was then reanalyzed using regression tests. The results showed that the application of various concentrations of NAA does not affect the growth of the number of roots of Kepok bananas var. Unti Sayang. The application of 2 ppm BAP is optimal for the growth of the number of shoots of Kepok bananas var. Unti Sayang. The interaction between 1 ppm NAA and BAP at 1-2 ppm produces the most optimal average shoot emergence time.
APPLICATION OF BENZYL ADENINE AND IAA ON THE GROWTH OF BANANA BARANGAN SUBCULTURE (Musa acuminata L.) Andriyana Setyawati; Samanhudi Samanhudi; Edi Purwanto; Retna Bandriyati Arniputri; Kevin Putra Setyawan; Muhammad Aji Cahyadi
Journal of Biodiversity and Biotechnology Vol 4, No 2 (2024)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v4i2.108497

Abstract

Barangan banana is a banana variety that has high economic value, but its production is still carried out conventionally, resulting in limited barangan bananas on the market. The use of tissue culture methods can quickly produce high-quality, disease-free plants regardless of seasonal conditions. The aim of this research is to determine the effect of providing the best ZPT IAA and BA for the growth of the Barangan banana planet. The research was conducted in August-October 2023 at the Biotechnology and Plant Physiology Laboratory, Sebelas Maret University. This research used a two-factor Completely Randomized Design (CRD) with 6 replications. The first factor was the use of ZPT Indole Acetic Acid (concentrations 0, 0.5, 1, and 1.5 ppm and the second factor was the use of ZPT Benzyl Adenine (0, 0.5, 1, 1.5 ppm). The variables observed were root emergence time, number of roots, root length, time of leaf emergence, number of leaves, time of shoot emergence, number of shoots, and height of shoots. The observed data were analyzed using Analysis of Variance (ANOVA) with a 5% level test and if there was a significant difference, it was continued with Duncan's Multiple Range Test (DMRT) at the level 5%. The results showed that giving 0.5 ppm IAA increased root length; 0.5 ppm IAA was given optimally for root formation; and 1 ppm IAA increased the shoot height of the Barangan banana plnalet. Giving BA 0.5 ppm root number; BA 1, 5 ppm produces the highest number of leaves; BA 1 ppm increases shoot height of barangan banana plantlets.
Co-Authors Andriyana Setyawati Ari Susilowati Arum Asri Trisnastuti Eviani Eviani Kevin Putra Setyawan Muhammad Aji Cahyadi Muji Rahayu Retna Bandriyati Arniputri Samanhudi Samanhudi