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Application of NAA and BA to Calotropis gigantea (L.) W.T. Aiton in vitro Muji Rahayu; Tyas Alivia Wulandari; Amalia Tetrani Sakya; Andriyana Setyawati; Sri Hartati; Samanhudi Samanhudi
Jurnal Ilmu Pertanian Vol 8, No 2 (2023): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.83664

Crown flower (Calotropis gigantea (L.)) is a weed plant potentially to be used as raw material for textile fibers yet the utilization is not optimal. Efforts to optimize the utilization of C. gigantea are needed. This research aimed to determine the appropriate concentration of plant growth regulators, including NAA (Naphthalene Acetic Acid) and BA (Benzyl Adenine) to stimulate optimal growth of C. gigantea to be used as the basis for in vitro propagation of C. gigantea. The research was arranged in a completely randomized design (CRD). Explants were planted on Murashige and Skoog (MS) medium with various concentrations of NAA (0; 0.5; 1; 1.5; and 2 ppm) and BA (0; 1; 2; 3; and 4 ppm). Based on the research result, the addition of 3 ppm BA followed by increasing the concentration of NAA to 1.5 ppm could increase the number of C. gigantea shoots, and giving 1 ppm BA followed by adding 1.5 to 2 ppm NAA increased shoot height. The best plantlet response was found in media with 0 ppm NAA and 4 ppm BA, which could produce an optimal shoot height of 5.9 cm and a large number of shoots of 5.67 shoots. Increasing NAA concentration retarded root formation and reduced the root length and number of leaves, while the medium without NAA gave the earliest root emergence of 11 days after planting, the number of leaves of 20 strands, and the longest root of 10.9 cm. Applying a single substance BA did not accelerate all the variables observed.

Application of NAA and BA to Calotropis gigantea (L.) W.T. Aiton in vitro Muji Rahayu; Tyas Alivia Wulandari; Amalia Tetrani Sakya; Andriyana Setyawati; Sri Hartati; Samanhudi Samanhudi
Jurnal Ilmu Pertanian Vol 8, No 2 (2023): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.83664

Crown flower (Calotropis gigantea (L.)) is a weed plant potentially to be used as raw material for textile fibers yet the utilization is not optimal. Efforts to optimize the utilization of C. gigantea are needed. This research aimed to determine the appropriate concentration of plant growth regulators, including NAA (Naphthalene Acetic Acid) and BA (Benzyl Adenine) to stimulate optimal growth of C. gigantea to be used as the basis for in vitro propagation of C. gigantea. The research was arranged in a completely randomized design (CRD). Explants were planted on Murashige and Skoog (MS) medium with various concentrations of NAA (0; 0.5; 1; 1.5; and 2 ppm) and BA (0; 1; 2; 3; and 4 ppm). Based on the research result, the addition of 3 ppm BA followed by increasing the concentration of NAA to 1.5 ppm could increase the number of C. gigantea shoots, and giving 1 ppm BA followed by adding 1.5 to 2 ppm NAA increased shoot height. The best plantlet response was found in media with 0 ppm NAA and 4 ppm BA, which could produce an optimal shoot height of 5.9 cm and a large number of shoots of 5.67 shoots. Increasing NAA concentration retarded root formation and reduced the root length and number of leaves, while the medium without NAA gave the earliest root emergence of 11 days after planting, the number of leaves of 20 strands, and the longest root of 10.9 cm. Applying a single substance BA did not accelerate all the variables observed.

Physiological Responses of Soybean Varieties to Different Types of Biochar Application Muji Rahayu; Andriyana Setyawati; Gani Cahyo Handoyo; Bramesty Laras Hanif; Fevi Catur Wulan Sari
PLANTA TROPIKA Vol. 13 No. 2 (2025)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v13i2.25382

Soybeans, a legume widely consumed in Indonesia, exhibit a comparatively low production rate. Therefore, initiatives to augment soybean production are essential, one of which is the utilization of biochar. Biochar has been extensively studied as a soil amendment that improves soil fertility and promotes plant growth. This study aimed to evaluate the physiological responses of soybean varieties to different types of biochar. The experiment used a factor randomized complete block design (RCBD) with three replications. The factor was three soybean varieties (Dena 1, Demas 1, and Gepak Kuning) and three biochar treatments (control, palm fibre, and rice hull). Data were analysed using analysis of variance (ANOVA), followed by Duncan’s Multiple Range Test (DMRT) at a 5% significance level. Results indicated that rice hull biochar improved net assimilation and relative growth rates by 1.95% and 1.59%, respectively, between the third and fifth weeks after planting. It also increased total dry weight by 1.37% and leaf area index by 1.72% compared to untreated plants. Among the varieties tested, Dena 1 yielded the highest total dry weight (1.41%) and leaf area index (1.53%), highlighting a promising synergy between this variety and rice hull biochar. These findings demonstrate that biochar type and soybean variety interact to determine physiological responses, suggesting that appropriate combinations may improve soybean productivity and resilience under field conditions.

Effect of Kinetin and 2,4-D Plant Growth Regulators on In Vitro Subculture Growth of Dendrobium stockelbuschii Okky Talitha; Kosei Yamauchi; Andriyana Setyawati; Muhammad Iqbal; Nanda Saskya
Agrosains : Jurnal Penelitian Agronomi Vol 27, No 2 (2025): Agrosains: Jurnal Penelitian Agronomi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agsjpa.v27i2.110517

Dendrobium orchids are a popular genus due to their adaptability and wide variation in flower shapes and colors. Conventional propagation methods face challenges in producing large quantities of high-quality seedlings. This study aimed to produce superior-quality seedlings through tissue culture techniques using ½ Murashige and Skoog (MS) medium supplemented with kinetin and 2,4-D to enhance growth. The research was conducted at the Plant Physiology and Biotechnology Laboratory, Sebelas Maret University, from February to July 2024. The experimental design used was a completely randomized design (CRD) with two factors. The first factor was kinetin concentration (0, 0.5, 1, 1.5, 2 ppm), and the second factor was 2,4-D concentration (0, 0.5, 1, 1.5, 2 ppm). A total of 25 treatment combinations were tested, each replicated three times, resulting in 75 experimental units. Observed parameters included the number of roots, plant height, number of shoots, and root length. Data were analyzed using analysis of variance (ANOVA) at a 5% significance level, followed by Duncan’s Multiple Range Test (DMRT) for mean separation. The results showed that kinetin had a significant effect on the number of roots and plant height, while 2,4-D significantly affected root length. No significant interaction was observed between the two factors.

Genetic Diversity of Three Types of Curcuma Using Molecular Sequence-Related Amplified Polymorphism Markers Andriyana Setyawati; Samanhudi Samanhudi; Zuraida Anniswa Bachtiar
Agrotechnology Research Journal Vol 9, No 1 (2025): Agrotechnology Research Journal
Publisher : Perkumpulan Agroteknologi/Agroekoteknologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agrotechresj.v9i1.84303

The genus Curcuma comprises numerous species with high medicinal value, many exhibiting morphological similarities that complicate taxonomic identification and hinder breeding efforts. This study aimed to elucidate the genetic diversity and relationships among three closely related species, Curcuma longa (turmeric), Curcuma purpurascens (temu tis), and Curcuma mangga using Sequence-Related Amplified Polymorphism (SRAP) markers. Genetic material was extracted from rhizome samples and amplified using five SRAP primer combinations, producing 56 loci with an average polymorphism percentage of 69.64%. The result showed a high genetic similarity (82%) between C. longa and C. purpurascens, whereas C. mangga exhibited a more distant relationship (55% similarity), indicating a narrower genetic base among the former two species. Additionally, total flavonoid content and antioxidant activity, with turmeric showing the highest flavonoid concentration (25.95% w/w), and C. mangga exhibiting the strongest antioxidant activity (42.12% wb). These findings highlight the effectiveness of SRAP markers in differentiating morphologically similar species and provide valuable genetic and phytochemical insights to support the conservation, breeding, and pharmacological development of Curcuma species. Integrating molecular and biochemical data underscores the potential for selecting superior varieties for both agricultural and medicinal applications.

Effect of Water Availability on the Growth and Secondary Metabolites of Zodia (Evodia suaveolens) Andriyana Setyawati; Edi Purwanto; Ahmad Yunus; Samanhudi Samanhudi; Amalia Tetrani Sakya; Muji Rahayu; Djoko Purnomo; Retna Bandriyati Arniputri; Gani Cahyo; Qonita Rahma Dwiyanti; Saat Egra; Okky Talitha; Alfida Muthi’ah
Agrosains : Jurnal Penelitian Agronomi Vol 25, No 2 (2023): Agrosains: Jurnal Penelitian Agronomi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agsjpa.v25i2.81528

Zodia are included in medicinal plants because it contains secondary metabolites such as flavonoids, tannins, alkaloids, steroids/triterpenoids that have many benefit and also as mosquito repellents. The growth and content of secondary metabolites in plants can be affected by environmental factors such as water availability. Research on the cultivation of this crop is still limited. Environmental modification in cultivation is needed to increase its secondary metabolites, and the study of the potential of this plant as medicine are necessary. This study aimed to determine the effect of water availability on the growth and secondary metabolites of zodia. The research was carried out in July-November 2021 in Greenhouse, Faculty of Agriculture, Universitas Sebelas Maret, Surakarta. The method used was a completely randomized design, one treatment factor with 4 levels namely, 100, 80, 60 and 40% of field capacity. Each treatment was repeated 6 times so that there were 24 experimental polybags. The data analysis used is Analysis of Variance, and the Duncan’s Multiple Range Test at the 5% level. The results showed that the availability of water at a level of 60% field capacity gave the best average growth parameters (plant height and number of leaves) among other treatments. The flavonoid content in each treatment showed the same average. The results of the GCMS (Gas Cromatography and Mass Spectroscopy) test of zodia leaves showed that there were dominant bioactive compounds, namely ethylbenzene, octadecamethyl cyclononasiloxane, pentadecanoid acid and heptadecanoid acid. It is suspected that the content of these compounds has the potential as antifungal, antibacterial, antioxidant and anticancer.

Budidaya Anggur dengan Sistem Pemantauan Berbasis IOT pada Kelompok Wanita Tani “Sekar Arum” Desa Genengan, Kecamatan Jumantono, Kabupaten Karanganyar Fitria Roviqowati; Ahmad Yunus; Edi Purwanto; Retna Bandriyati Arniputri; Samanhudi Samanhudi; Muji Rahayu; Gani Cahyo Handoyo; Andriyana Setyawati; Iswahyudi Iswahyudi; Joko Prihanto
SEMAR (Jurnal Ilmu Pengetahuan, Teknologi, dan Seni bagi Masyarakat) Vol 15, No 1 (2026): Mei
Publisher : LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/semar.v15i1.109864

Desa Genengan memiliki potensi geografis yang mendukung budidaya anggur, namun pengembangannya oleh Kelompok Wanita Tani (KWT) “Sekar Arum” terkendala oleh keterbatasan pengetahuan teknik budidaya modern dan sistem pemantauan lingkungan yang masih konvensional. Program pengabdian ini bertujuan untuk: (1) meningkatkan pengetahuan dan keterampilan anggota KWT mengenai budidaya anggur dan teknik grafting, (2) mengimplementasikan sistem pemantauan lingkungan berbasis Internet of Things (IoT), dan (3) menganalisis efektivitas pelatihan serta kendala adopsi teknologi. Metode pelaksanaan meliputi pelatihan teoritis dan praktik, implementasi demplot, serta pemasangan perangkat IoT yang memantau suhu, kelembaban tanah, intensitas cahaya, dan pH. Evaluasi menggunakan pretest-posttest dan kuesioner kepuasan. Hasil menunjukkan partisipasi 22 anggota (100%). Terjadi peningkatan pengetahuan yang signifikan dengan nilai rata-rata pretest 71,03 menjadi posttest 93,05 (gain 22,02 poin). Tingkat keberhasilan praktek grafting mencapai 75%. Berdasarkan angket yang telah dibagian diperoleh data bahwa keseluruhan peserta menyatakan materi yang disampaikan bermanfaat. Tingkat ketertarikan pada kegiatan 72,2% menyatakan sangat menarik serta 22,8% menyatakan menarik. Keseluruhan peserta menyatakan tertarik untuk melakukan budidaya anggur di rumah. Sistem IoT beroperasi dengan baik, meski mengalami kendala konektivitas internet. Analisis mengungkap penerimaan yang lebih tinggi terhadap materi budidaya konvensional dibandingkan teknologi IoT, serta ketergantungan pada infrastruktur digital sebagai penghambat utama. Program ini berhasil meningkatkan kapasitas teknis kelompok dan membuka jalan menuju pertanian presisi. Keberlanjutan memerlukan pendampingan lanjutan, penguatan aspek bisnis, dan solusi infrastruktur komunikasi yang adaptif.Kata kunci: budidaya anggur; IoT; kelompok wanita tani; pertanian presisi; pemberdayaan masyarakat

The Effect of Naphtaleine Acetic Acid ND Benzyl Amino Purine on the Subculture of Kepok Unti Sayang Banana Samanhudi Samanhudi; Andriyana Setyawati; Edi Purwanto; Muji Rahayu; Eviani Eviani; Muhammad Aji Cahyadi
Journal of Biodiversity and Biotechnology Vol 5, No 1 (2025)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v5i1.108513

The Kepok Unti Sayang variety is a superior type of banana that is resistant to fusarium wilt. The high demand for Kepok Unti Sayang needs to be balanced with good seed production. One of the methods used for multiplying superior seeds of Kepok Unti Sayang is through tissue culture. This research aims to obtain the optimal concentration of Naphtaleine Acetic Acid (NAA) in the subculture media for the growth of root numbers in the Kepok Unti Sayang Banana. The second objective is to determine the optimal concentration of Benzyl Amino Purine (BAP) in the subculture medium for shoot number growth in Kepok Unti Sayang bananas. Another goal is to identify the best treatment combination that can produce the highest number of shoots in Kepok Unti Sayang bananas. The research was conducted at the Laboratory of Plant Physiology and Biotechnology at Sebelas Maret University from February to July 2025. The study was conducted with 4 levels of NAA treatment: 0; 0.5; 1; and 1.5 ppm. The BAP treatment was administered at 4 levels: 0; 1; 2; and 3 ppm. The variables observed in this study were the time of shoot emergence, number of shoots, time of root emergence, root length, and final height of the planlet. Data from each variable were then analyzed using analysis of variance (ANOVA) with an F-test at a significance level of 95%. Significantly different data were then followed by Duncan's Multiple Range Test (DMRT) at a significance level of 95%. The significant combination of NAA and BAP on the observed variables was then reanalyzed using regression tests. The results showed that the application of various concentrations of NAA does not affect the growth of the number of roots of Kepok bananas var. Unti Sayang. The application of 2 ppm BAP is optimal for the growth of the number of shoots of Kepok bananas var. Unti Sayang. The interaction between 1 ppm NAA and BAP at 1-2 ppm produces the most optimal average shoot emergence time.

APPLICATION OF BENZYL ADENINE AND IAA ON THE GROWTH OF BANANA BARANGAN SUBCULTURE (Musa acuminata L.) Andriyana Setyawati; Samanhudi Samanhudi; Edi Purwanto; Retna Bandriyati Arniputri; Kevin Putra Setyawan; Muhammad Aji Cahyadi
Journal of Biodiversity and Biotechnology Vol 4, No 2 (2024)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v4i2.108497

Barangan banana is a banana variety that has high economic value, but its production is still carried out conventionally, resulting in limited barangan bananas on the market. The use of tissue culture methods can quickly produce high-quality, disease-free plants regardless of seasonal conditions. The aim of this research is to determine the effect of providing the best ZPT IAA and BA for the growth of the Barangan banana planet. The research was conducted in August-October 2023 at the Biotechnology and Plant Physiology Laboratory, Sebelas Maret University. This research used a two-factor Completely Randomized Design (CRD) with 6 replications. The first factor was the use of ZPT Indole Acetic Acid (concentrations 0, 0.5, 1, and 1.5 ppm and the second factor was the use of ZPT Benzyl Adenine (0, 0.5, 1, 1.5 ppm). The variables observed were root emergence time, number of roots, root length, time of leaf emergence, number of leaves, time of shoot emergence, number of shoots, and height of shoots. The observed data were analyzed using Analysis of Variance (ANOVA) with a 5% level test and if there was a significant difference, it was continued with Duncan's Multiple Range Test (DMRT) at the level 5%. The results showed that giving 0.5 ppm IAA increased root length; 0.5 ppm IAA was given optimally for root formation; and 1 ppm IAA increased the shoot height of the Barangan banana plnalet. Giving BA 0.5 ppm root number; BA 1, 5 ppm produces the highest number of leaves; BA 1 ppm increases shoot height of barangan banana plantlets.

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