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All Journal Biogenesis: Jurnal Ilmiah Biologi Inovasi Kesehatan Global
Septian Tri Wicaksono
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Identification of endophytic fungi in nutgrass (Cyperus rotundus L.) as solubilizing phosphate and indole-3-acetic acid producers Nur Kusmiyati; Septian Tri Wicaksono; Durrotul Maknuna
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.21216

Abstract

Low phosphate content in the soil causes insufficient plant needs. Meanwhile, endophytic fungi in nutgrass have great potential as a phosphate solvent and produce indole-3-acetic acid (IAA). Therefore, this study aims to determine the solubilizing phosphate and IAA production by the endophytic fungi of nutgrass and identify the isolates based on rDNA-ITS sequences. Endophytic fungi isolates were cultured in 10 ml of Pikovskaya broth media with Ca5(PO4)3 as the inorganic phosphate source. The PCR results were analyzed using 1.5% agarose gel electrophoresis followed by sequence analysis. The isolation and purification results showed five isolates coded URT1, URT2, URT3, URT4, and URT5, while the solubilizing phosphate levels ranged from 54.03-87.83 ppm, with the highest levels found in the URT4 isolate. Furthermore, the IAA levels ranged from 5.58-45.50 ppm, with the highest levels produced by the URT1 isolate. The molecular analysis with rDNA-ITS sequences showed that URT4 had 97.42% similarity to Aspergillus terreus species, while UTR1 had 100% similarity to Fusarium oxysporum species. Based on the results, the endophytic fungi of nutgrass from A. terreus and F. oxysporum species have great potential as biofertilizers due to the high solubilizing phosphate and IAA levels.
Efektivitas Intervensi Fisioterapi dalam Mengurangi Nyeri dan Meningkatkan ROM pada Lansia Penderita Osteoarthtritis Genu di RST. Soedjono Magelang Zain, Siti Azzura; Razzaq, Aliyah Nadhifah; Indraprastha, Annya Shakty; Salsabila, Anida Nasywa; Septian Tri Wicaksono; Arjuna Fatkhur Roziq Pahlavi; Arif Pristianto; Munawar Munawar
Inovasi Kesehatan Global Vol. 2 No. 4 (2025): November: Inovasi Kesehatan Global
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62383/ikg.v2i4.2372

Abstract

Background: Knee osteoarthritis is one of the most common degenerative conditions in older adults, often causing pain and limited range of motion (ROM), which negatively affects quality of life. Physiotherapy management plays an important role in reducing pain, improving flexibility, and maintaining joint function. Objective: This study aims to evaluate the effectiveness of physiotherapy interventions in reducing pain and improving ROM in elderly patients with knee osteoarthritis. Method: A case study was conducted on a 63-year-old patient at Dr. Soedjono Level II Hospital, Magelang, who underwent three therapy sessions consisting of Infrared (IR), Transcutaneous Electrical Nerve Stimulation (TENS), Proprioceptive Neuromuscular Facilitation (PNF) with the hold-relax technique, and static cycling. Assessments were carried out using the Numeric Rating Scale (NRS), Likert Gait Scale (LGS), and the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC). Results: The findings showed a significant reduction in pain on palpation (from 4/10 to 1/10), improvement in knee flexion ROM (from 110° to 135°), and functional enhancement indicated by a decrease in WOMAC scores from 39.58% to 27.08%. Conclusion: These results suggest that structured physiotherapy interventions provide therapeutic benefits in reducing pain, improving joint flexibility, and enhancing functional ability in elderly patients with knee osteoarthritis.
Co-Authors Arif Pristianto Arjuna Fatkhur Roziq Pahlavi Durrotul Maknuna Indraprastha, Annya Shakty Munawar Munawar Nur Kusmiyati Razzaq, Aliyah Nadhifah Salsabila, Anida Nasywa Zain, Siti Azzura