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All Journal Indonesian Journal of Agricultural Science Jurnal Penelitian Tanaman Industri Jurnal Penelitian Tanaman Industri (Littri)
I. Darwati
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Agriculture, Biological Sciences & Forestry Industrial & Manufacturing Engineering

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REGENERATION OF Pimpinella pruatjan THROUGH SOMATIC EMBRYOGENESIS I. Roostika; R. Purnamaningsih; I. Darwati; I. Mariska
Indonesian Journal of Agricultural Science Vol 8, No 2 (2007): October 2007
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v8n2.2007.p60-66

Abstract

Pruatjan (Pimpinella pruatjan Molk.) is an Indonesian endangered plant which has various medicinal properties such as aphrodisiac, diuretic, and tonic. The plant is commonly harvested from its natural habitat, therefore it becomes endangered. Regeneration of pruatjan through organogenesis has been studied, but its shoot multiplication was very low (5 shoots per explant). The study aimed to investigate the best regeneration technique of pruatjan through somatic embryogenesis. This research was conducted at the tissue culture laboratory, Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development in 2004-2005. Callus formation of pruatjan was induced from the petioles and leaves in Driver and Kuniyaki’s (DKW) based medium containing 2,4-D combined with picloram at the level of 0.5, 1.0, 1.5, and 1.5 ppm. Embryogenic calli were then transferred into embryo development medium in two ways. First, they were directly transferred into media containing IBA/NAA at the level of 0.5, 1.0, and 1.5 ppm. Second, they were indirectly transferred into media containing 2.0 ppm 2,4-D and 0.3% casein hydrolysate prior to the IBA/ NAA media. Parameters evaluated were fresh weight, dry weight, time initiation of embryogenic callus formation, and total number of embryos. The result showed that calli of pruatjan were successfully induced from the petioles and leaves. The best calli were induced from the leaves in the DKW medium containing 2.0 ppm 2,4-D and 0.5 ppm picloram. Embryo development of the calli was best if they were first grown in the media containing 2.0 ppm 2,4-D and 0.3% casein hydrolysate then transferred to the IBA/NAA media. The total number of somatic embryos was counted up to 103 on the medium containing 1.5 ppm IBA. This study indicated that pruatjan somatic embryogenesis regeneration required three different media, i.e. for callus induction, development and maturation, and for germination.
PENGARUH PEMUPUKAN TERHADAP PERTUMBUHAN, PRODUKSI, DAN MUTU TANAMAN TIMI (Thymus vulgaris L.) RAHARDJO RAHARDJO; I. DARWATI; H. NURHAYATI
Jurnal Penelitian Tanaman Industri Vol 20, No 4 (2014): Desember 2014
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v20n4.2014.195-202

Abstract

ABSTRAK Tanaman   timi   banyak   dibudidayakan   di   Indonesia   karena bermanfaat  untuk  kesehatan.  Kebutuhan  hara  N,  P,  dan  K  penting diketahui  untuk  mendukung  budidayanya.  Penelitian  bertujuan  untuk mengetahui respon pemupukan terhadap pertumbuhan, produksi, mutu simplisia, dan serapan hara tanaman timi. Penelitian dilaksanakan di KP. Manoko  (1200  m  dpl)  pada  bulan  Januari  sampai  Desember 2013. Penelitian menggunakan rancangan acak kelompok dengan empat ulangan. Perlakuan pemupukan: (1) kontrol (tanpa pupuk); (2) 20 ton/ha pupuk kandang; (3) 20 ton/ha pupuk kandang + urea, SP36, dan KCl masing- masing 50 kg/ha; (4) 20 ton/ha pupuk kandang + urea, SP36, dan KCl masing-masing 75 kg/ha; (5) 20 ton/ha pupuk kandang + urea, SP36, dan KCl masing-masing 100 kg/ha; (6) 20 ton/ha pupuk kandang + urea, SP36, dan KCl masing-masing 125 kg/ha; (7) 20 ton/ha pupuk kandang + urea, SP36, dan KCl masing-masing 150 kg/ha; dan (8) Urea, SP36, dan KCl masing-masing 150 kg/ha. Peubah yang diamati: tinggi, bobot segar dan kering, produksi herba kering, mutu simplisia, serta kadar hara N, P, dan K tanaman. Produksi herba tertinggi (3,93 ton/ha) diperoleh pada perlakuan urea, SP36, dan KCl masing-masing 150 kg/ha. Peningkatan SP36 menjadi 150 kg/ha yang diikuti oleh urea dan KCl masing-masing 150 kg/ha tanpa pupuk kandang, mampu meningkatkan herba kering timi 44-88%. Kadar thymol  tertinggi (70,90%)  dicapai  pada  perlakuan 20  ton/ha  pupuk kandang +  urea,  SP36,  dan  KCl  masing-masing 150  kg/ha,  namun menghasilkan kadar minyak atsiri terendah (0,30%). Sebaliknya, kadar thymol terendah (43,99%) dicapai pada perlakuan tanpa pupuk, tetapi memiliki kadar minyak atsiri tertinggi (0,92%). Kata kunci:  Thymus  vulgaris  L.,  pemupukan,  pertumbuhan,  produksi,             kualitas The Effect of Fertilizer on Growth, Yield, and Quality of Thyme (Thymus vulgaris L.)  ABSTRACT   Thyme has been cultivating in Indonesia because of  its benefits for health. Thyme nutrients requirement is important to support its cultivation. This study is aimed to evaluate thyme responses to fertilizer. The research was  conducted  at  Manoko  Experimental  Garden (1200  m  asl)  from January to December 2013, arranged in randomized block design with four replications. Fertilization treatments: (1) control (no fertilizer); (2) 20 tonnes/kg manure; (3) 20 tonnes/ha manure + 50 kg/ha of each urea, SP36, and KCl; (4) 20 tonnes/kg manure + 75 kg/ha of each urea, SP36, and KCl; (5) 20 tonnes/kg manure + 100 kg/ha of each urea, SP36, and KCl; (6) 20 tonnes/kg manure + 125 kg/ha of each urea, SP36, and KCl; (7) 20 tonnes/kg manure + 150 kg/ha of each urea, SP36, and KCl; and (8) 150 kg/ha of each urea, SP36, and KCl. Parameters observed: plant height, fresh and dry weight per plant, yield of dry herb, simplicia quality, and N, P, and K contents. Treatment 150 kg/ha of each urea, SP36, and KCl was produced the highest yield (3.93 tonnes/ha). The increase of SP36 until 25 kg/ha combined with 150 kg/ha urea and KCl could enhance yield of dry herb 44-88%. The highest thymol content (70.90%) was obtained from treatment 20 tonnes/kg manure + 150 kg/ha of each urea, SP36, and KCl, but it produced the lowest essential oil content (0.30%). Contrarily, control treatment produced the highest essential oil content (0.92%) but gave the lowest thymol content (43.99%).Key words: Thymus vulgaris L., fertilization, growth, yield, quality
PENGARUH PEMUPUKAN TERHADAP PERTUMBUHAN, PRODUKSI, DAN MUTU TANAMAN TIMI (Thymus vulgaris L.) RAHARDJO RAHARDJO; I. DARWATI; H. NURHAYATI
Jurnal Penelitian Tanaman Industri Vol 20, No 4 (2014): Desember 2014
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v20n4.2014.195-202

Abstract

ABSTRAK Tanaman   timi   banyak   dibudidayakan   di   Indonesia   karena bermanfaat  untuk  kesehatan.  Kebutuhan  hara  N,  P,  dan  K  penting diketahui  untuk  mendukung  budidayanya.  Penelitian  bertujuan  untuk mengetahui respon pemupukan terhadap pertumbuhan, produksi, mutu simplisia, dan serapan hara tanaman timi. Penelitian dilaksanakan di KP. Manoko  (1200  m  dpl)  pada  bulan  Januari  sampai  Desember 2013. Penelitian menggunakan rancangan acak kelompok dengan empat ulangan. Perlakuan pemupukan: (1) kontrol (tanpa pupuk); (2) 20 ton/ha pupuk kandang; (3) 20 ton/ha pupuk kandang + urea, SP36, dan KCl masing- masing 50 kg/ha; (4) 20 ton/ha pupuk kandang + urea, SP36, dan KCl masing-masing 75 kg/ha; (5) 20 ton/ha pupuk kandang + urea, SP36, dan KCl masing-masing 100 kg/ha; (6) 20 ton/ha pupuk kandang + urea, SP36, dan KCl masing-masing 125 kg/ha; (7) 20 ton/ha pupuk kandang + urea, SP36, dan KCl masing-masing 150 kg/ha; dan (8) Urea, SP36, dan KCl masing-masing 150 kg/ha. Peubah yang diamati: tinggi, bobot segar dan kering, produksi herba kering, mutu simplisia, serta kadar hara N, P, dan K tanaman. Produksi herba tertinggi (3,93 ton/ha) diperoleh pada perlakuan urea, SP36, dan KCl masing-masing 150 kg/ha. Peningkatan SP36 menjadi 150 kg/ha yang diikuti oleh urea dan KCl masing-masing 150 kg/ha tanpa pupuk kandang, mampu meningkatkan herba kering timi 44-88%. Kadar thymol  tertinggi (70,90%)  dicapai  pada  perlakuan 20  ton/ha  pupuk kandang +  urea,  SP36,  dan  KCl  masing-masing 150  kg/ha,  namun menghasilkan kadar minyak atsiri terendah (0,30%). Sebaliknya, kadar thymol terendah (43,99%) dicapai pada perlakuan tanpa pupuk, tetapi memiliki kadar minyak atsiri tertinggi (0,92%). Kata kunci:  Thymus  vulgaris  L.,  pemupukan,  pertumbuhan,  produksi,             kualitas The Effect of Fertilizer on Growth, Yield, and Quality of Thyme (Thymus vulgaris L.)  ABSTRACT   Thyme has been cultivating in Indonesia because of  its benefits for health. Thyme nutrients requirement is important to support its cultivation. This study is aimed to evaluate thyme responses to fertilizer. The research was  conducted  at  Manoko  Experimental  Garden (1200  m  asl)  from January to December 2013, arranged in randomized block design with four replications. Fertilization treatments: (1) control (no fertilizer); (2) 20 tonnes/kg manure; (3) 20 tonnes/ha manure + 50 kg/ha of each urea, SP36, and KCl; (4) 20 tonnes/kg manure + 75 kg/ha of each urea, SP36, and KCl; (5) 20 tonnes/kg manure + 100 kg/ha of each urea, SP36, and KCl; (6) 20 tonnes/kg manure + 125 kg/ha of each urea, SP36, and KCl; (7) 20 tonnes/kg manure + 150 kg/ha of each urea, SP36, and KCl; and (8) 150 kg/ha of each urea, SP36, and KCl. Parameters observed: plant height, fresh and dry weight per plant, yield of dry herb, simplicia quality, and N, P, and K contents. Treatment 150 kg/ha of each urea, SP36, and KCl was produced the highest yield (3.93 tonnes/ha). The increase of SP36 until 25 kg/ha combined with 150 kg/ha urea and KCl could enhance yield of dry herb 44-88%. The highest thymol content (70.90%) was obtained from treatment 20 tonnes/kg manure + 150 kg/ha of each urea, SP36, and KCl, but it produced the lowest essential oil content (0.30%). Contrarily, control treatment produced the highest essential oil content (0.92%) but gave the lowest thymol content (43.99%).Key words: Thymus vulgaris L., fertilization, growth, yield, quality
Co-Authors H. NURHAYATI I. Mariska I. Roostika RAGAPADMI PURNAMANINGSIH RAHARDJO RAHARDJO