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I. Mariska
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REGENERATION OF Pimpinella pruatjan THROUGH SOMATIC EMBRYOGENESIS Roostika, I.; Purnamaningsih, R.; Darwati, I.; Mariska, I.
Indonesian Journal of Agricultural Science Vol 8, No 2 (2007): October 2007
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Abstract

Pruatjan (Pimpinella pruatjan Molk.) is an Indonesian endangeredplant which has various medicinal properties such asaphrodisiac, diuretic, and tonic. The plant is commonly harvestedfrom its natural habitat, therefore it becomes endangered. Regenerationof pruatjan through organogenesis has been studied,but its shoot multiplication was very low (5 shoots per explant).The study aimed to investigate the best regeneration techniqueof pruatjan through somatic embryogenesis. This research wasconducted at the tissue culture laboratory, Indonesian Centerfor Agricultural Biotechnology and Genetic Resources Researchand Development in 2004-2005. Callus formation of pruatjanwas induced from the petioles and leaves in Driver andKuniyaki’s (DKW) based medium containing 2,4-D combinedwith picloram at the level of 0.5, 1.0, 1.5, and 1.5 ppm. Embryogeniccalli were then transferred into embryo developmentmedium in two ways. First, they were directly transferred intomedia containing IBA/NAA at the level of 0.5, 1.0, and 1.5 ppm.Second, they were indirectly transferred into media containing2.0 ppm 2,4-D and 0.3% casein hydrolysate prior to the IBA/NAA media. Parameters evaluated were fresh weight, dryweight, time initiation of embryogenic callus formation, andtotal number of embryos. The result showed that calli ofpruatjan were successfully induced from the petioles and leaves.The best calli were induced from the leaves in the DKWmedium containing 2.0 ppm 2,4-D and 0.5 ppm picloram.Embryo development of the calli was best if they were firstgrown in the media containing 2.0 ppm 2,4-D and 0.3% caseinhydrolysate then transferred to the IBA/NAA media. The totalnumber of somatic embryos was counted up to 103 on themedium containing 1.5 ppm IBA. This study indicated thatpruatjan somatic embryogenesis regeneration required threedifferent media, i.e. for callus induction, development andmaturation, and for germination.
REGENERATION OF Pimpinella pruatjan THROUGH SOMATIC EMBRYOGENESIS I. Roostika; R. Purnamaningsih; I. Darwati; I. Mariska
Indonesian Journal of Agricultural Science Vol 8, No 2 (2007): October 2007
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v8n2.2007.p60-66

Abstract

Pruatjan (Pimpinella pruatjan Molk.) is an Indonesian endangered plant which has various medicinal properties such as aphrodisiac, diuretic, and tonic. The plant is commonly harvested from its natural habitat, therefore it becomes endangered. Regeneration of pruatjan through organogenesis has been studied, but its shoot multiplication was very low (5 shoots per explant). The study aimed to investigate the best regeneration technique of pruatjan through somatic embryogenesis. This research was conducted at the tissue culture laboratory, Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development in 2004-2005. Callus formation of pruatjan was induced from the petioles and leaves in Driver and Kuniyaki’s (DKW) based medium containing 2,4-D combined with picloram at the level of 0.5, 1.0, 1.5, and 1.5 ppm. Embryogenic calli were then transferred into embryo development medium in two ways. First, they were directly transferred into media containing IBA/NAA at the level of 0.5, 1.0, and 1.5 ppm. Second, they were indirectly transferred into media containing 2.0 ppm 2,4-D and 0.3% casein hydrolysate prior to the IBA/ NAA media. Parameters evaluated were fresh weight, dry weight, time initiation of embryogenic callus formation, and total number of embryos. The result showed that calli of pruatjan were successfully induced from the petioles and leaves. The best calli were induced from the leaves in the DKW medium containing 2.0 ppm 2,4-D and 0.5 ppm picloram. Embryo development of the calli was best if they were first grown in the media containing 2.0 ppm 2,4-D and 0.3% casein hydrolysate then transferred to the IBA/NAA media. The total number of somatic embryos was counted up to 103 on the medium containing 1.5 ppm IBA. This study indicated that pruatjan somatic embryogenesis regeneration required three different media, i.e. for callus induction, development and maturation, and for germination.
Pembentukan Pisang Ambon Toleran Terhadap Penyakit Layu Fusarium Melalui Variasi Somaklonal M. Kosmiatin; I. Mariska; I. Roostika; E. Gati
Zuriat Vol 17, No 1 (2006)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v17i1.6760

Abstract

Pisang Ambon (Musa paradisiaca) adalah salah satu jenis pisang yang populer dan berkembang di Indonesia. Perkembangan pertanaman ini juga diikuti dengan perkembangan penyakit yang menyerang dan berakibat fatal. Salah satu penyakti yang sangat pesat perkembangannya adalah penyakit layu fusarium yang disebabkan oleh Fusarium oxysporum Schlect f. sp. Cubense. Pisang ambon merupakan jenis pisang yang sangat rentan terhadap penyakit tersebut. Hingga saat ini usaha pemuliaan pisang sulit dilakukan karena keragaman genetiknya rendah. Peningkatan keragaman dapat diperoleh dari sel-sel somatik yang pada dasarnya merupakan individu yang berkemampuan untuk beregenerasi membentuk tanaman lengkap. Induksi mutasi untuk meningkatkan keragaman somaklonal dan diikuti dengan seleksi efektif dilakukan secara in vitro karena perubahannya dapat ditujukan pada tingkat sel dan hanya pada sifat-sifat tertentu. Penelitian ini bertujuan untuk mendapatkan tanaman pisang ambon kuning yang tahan penyakit layu fusarium. Penelitian dilakukan dalam 2 tahap: 1. Induksi kalus, peningkatan keragaman dan seleksi in vitro, 2. Aklimatisasi dan pengujian bibit dengan isolat F. oxysporum di kamar dan di lokasi endemik. Hasil penelitian menunjukkan bahwa eksplan pisang ambon kuning berespon baik untuk pengkalusan. Kalus-kalus yang diradiasi sinar gamma dan diseleksi pada media dengan toksin asam fusarat daya regenerasinya menurun. Untuk meningkatkan kemampuan regenerasinya digunakan media dengan peningkatan konsentrasi thidiazuron. Bibit yang berasal dari kalus yang bertahan hidup setelah diradiasi dan diseleksi dengan toksin ketahanannya berkorelasi positif ketika diuji dengan isolat F. oxysporum Schlect f. sp. Cubense. Korelasi positif juga terlihat ketika tanaman diuji di lokasi endemik.
Co-Authors E. Gati I. Darwati I. Darwati I. Roostika I. Roostika I. Roostika M. Kosmiatin RAGAPADMI PURNAMANINGSIH