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All Journal Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences BERITA BIOLOGI

Djaenudin Gholib

Balai Penelitian Tanaman Rempah dan Obat

Author-ID : 269302

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Veterinary

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Dermatophytes Fungi Trichophyton Verrucosum A Causative Agent of Ringworm Disease on Cattle Gholib, Djaenudin; Rachmawati, S
Indonesian Bulletin of Animal and Veterinary Sciences Vol 20, No 1 (2010)
Publisher : Indonesian Animal Sciences Society

Ringworm is a superficial mycosis which affected surface parts of the body such as skin, hair, nail or horn. These parts are rich of keratine that required for the fungi to grow. The disease affects both animals and human (zoonosis), and it results in hair loss, crusted of skin, swelling, erythema and itchy. The disease is considered as important because it affects health condition and animal production. The causative agent is fungal dermatophytes group especially Trichophyton verrucosum. Cases occurred in Indonesia and first officially reported and published in 1980 on imported dairy cows from Australia, and a causative agent was identified as Trichophyton verrucosum. Recently, the same cases occurred in young dairy calves and lactating cattle. Pathogenity test in rabbits by artificial infection revealed the growth of the colonies. Post infection and vaccination provide resistency on cattle. Therefore beside therapeutic method, the vaccination program is useful to be included in prevention of the disease. Key words: Trichophyton verrucosum, ringworm, cattle

Development of serological technique for examination of aspergillosis in chicken Gholib, Djaenudin
Indonesian Journal of Animal and Veterinary Sciences Vol 10, No 2 (2005)
Publisher : Indonesian Animal Sciences Society

Aspergillosis detection by using serological method has not been reported in Indonesia. In this case, a study was conducted, by using mycelium extract of A. fumigatus as the antigen. Rabbits and chickens were injected with the antigen to produce positive serum (antiserum). The antigen and antiserum were tested serologicaly by Immunodiffusion/Agar Gel Precipitation (AGP), ELISA and Immunoblot. Chicken serum of broiler and layer collected from field were also included in the test. All positive serum of the experimentally animals gave positive results with all methods of serological tests. No bands of precipitation reaction in AGP test with chicken serum from the field. Both chicken and rabbit positive serum with ELISA test showed high Optical Density (OD), while field chicken serum from broiler commonly gave lower OD compared to layer. Immunoblot test of chicken positive serum showed bands of reaction with the antigen in nitrocellulose membrane, approximately on 33, 38, 44, 52, 70, 77, 97, and 110 kDa, meanwhile field chicken serum with high OD in ELISA test, showed bands approximately on 16, 18, 33, 38, 44, 47, 52, 70, 77, 84, 97, and 110 kDa. It means that the field chicken serum contain immunoglobulin molecules has spesific antibody of aspergillus antigen. It is concluded that the ELISA test can be used for screening on chicken aspergillosis in serological methode. Â Â Key Words: Antigen, Aspergillus fumigatus, Serology, Chicken

Cases of aspergillosis in Javan Hawk-eagle (Spizaetus bartelst): Isolation of the causative agents and the pathological changes of the diseas Hastiono, Sukardi; Damayanti, Rini; Gholib, Djaenudin
Indonesian Journal of Animal and Veterinary Sciences Vol 1, No 2 (1995)
Publisher : Indonesian Animal Sciences Society

Two cases of aspergillosis in Javan Hawk-eagle (Spizaetus banelsi) from Safari Garden of Indonesia, Cisarua, Bogor, have been reported . Specimens of lungs, airsacs, livers, spleens, kidneys, and intestines were examined mycologically and pathologically . Mycological examination revealed in the isolation of the causative agents from lungs and airsacs, ie . Aspergillus niger from one bird, and Aspergillusfianigarus from the other one . Pathological anatomy examination showed formation of yellowish white miliary nodule lesions, especially in the lungs and airsacs, while histopathological examination showed congestion and granulomatous tissue formation in the lungs, ie . specific lesion of mycotic infection with necrotic zone in the centre, surrounded by lymphocytes, macrophages, fibrin, and fungal hyphae . Pathological changes of the other organs were also described . These findings were the first officially description of aspergillosis cases in these carnivorous birds . Key words: Javan Hawk-eagle, Spizaetus battelsi, aspergillosis, Aspergillus niger, Aspergillus fumigatus

The preparation of Dermatophilus congolensis antigen and its testing by means of immunodiffusion test and electrophoresis Gholib, Djaenudin; ., Subiyanto
Indonesian Journal of Animal and Veterinary Sciences Vol 3, No 3 (1998)
Publisher : Indonesian Animal Sciences Society

The filtrate antigen ofDermatophilus congolensis was prepared based on the Makinde method, whereas the whole cell antigen was based on the Bida and Kelley method. Filtrate antigen of Dermatophilus congolensis has been tested with positive serum from experimental animals, guinea pigs and sheep by means of immurrodiffitsion test and electrophoresis . Positive serum was produced by inoculation of whole cell antigen of D. congolensis to the animals . The results showed that the immunodiffusion test resulted in one and two precipitation lines with positive serum of sheep and guinea pigs respectively. Electrophoresis SDS-PAGE presented about 8 bands with molecular weight in the range from above 30 kD to more than 94 kD. The bands were then transferred into nitrocellulose membrane and gave positive reaction with positive serum from sheep. Â Key words : Antigen, Dermatophilus congolensis, immunodiffusion, electrophoresis

CENDAWAN PENYEBAB ABORTUS DALAM ALAT REPRODUKSI SAPI BETINA [Fungi Causing Abortion in Reproductive Track of Cow] Gholib, Djaenudin; Ahmad, Riza Zainuddin
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The study on cases of abortion in cow caused by fungi have never been reported so far in Indonesia. The aim of this research is to get prevalence of cows have not and have aborted, both have reproductive disorder, and health cattles associated with percentage of fungi isolated.The study included both field survey and laboratory examination of samples. The samples of vaginal fluid were collected by using cotton swabs in cow which had suffered abortion or cow had repeated breeding, as well as freeze dried semen for artificial insemination (AI), and also examining the occurrence of abortion in cattle. The samples were collected in Sukabumi and Bandung, and then cultured in Sabouraud Dextrose Agar by stretch method, followed by incubation at 37oC. Number of samples collected consisted of 56 samples of cattle which had suffered from abortion and cow that had reproductive problems; 38 samples as control; five samples of freeze dried semen. There were no cases of abortion observed when field survey was conducted, therefore organ samples were not available for pathological and histophathological examination. Further examination on growth rate of yeast showed 20% of yeast presented in cows with abortion, 17% in cows with reproductive problem and 14% in normal cattle. On the other hand,there were samples that did not show any growth of yeast recorded f rom cows with abortion (15%)cattle with reproductive problem (7%) and normal cows (27%). Meanwhile, only twowith 2 yeast growth observed in semen samples. It was concluded that samples from cattles with reproductive problems (with and without aborted experiences) showed higher number of growth of yeasts compared with normal cow (P<0,05). Freeze dried semen were having risk of contamination by yeasts. There was no different on growth rate of fungi between reproductive problem and with abortion cow (P>0,05).

UJI DAYA HAMBAT DAUN SENGGANI (Melastoma malabathricum L.) TERHADAP Trichophyton mentagrophytees DAN Candida albicans Gholib, Djaenudin
BERITA BIOLOGI Vol 9, No 5 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The in vitro study of" inhibition effect of senggani leaves (Melastoma malabathricum L.) ethanol extract on Trichophyton mentagrophytees and Candida albicans was conducted by diffusion and dilution method. The extract was diluted into 5%, 10%,15% and 20% for diffusion test, and 1%, 2%, 3%, 4% and 5% for dilution test on T. mentagrophytees, and 2%, 4%, 6%, 8%, and 10% on C. albicans. The results showed that the extract inhibited fungal growth. Agar diffusion test (agar wheel) was observed by diameter of inhibition zone around the wheel. At 20% extract dilution, the diameter mean was estimated 30 mm on T. mentagrophytees, and 21 mm on C. albicans. In dilution test, the result showed Minimal Inhibition Concentration (MIC) of the extract was 3% on T. mentagrophytees, and 6% on C. albicans. It is concluded that the extract effect of inhibition on T. mentagrophytees is greater than that on C. albicans.

Co-Authors . Darmono Riza Zainuddin Ahmad S Rachmawati Sembiring, Rinawati Subiyanto . Sukardi Hastiono

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