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Sudarsono Sudarsono
Departemen Agronomi Dan Hortikultura (AGRO-HORT), Fakultas Pertanian, Institut Pertanian Bogor, Jl. Meranti, Kampus IPB Darmaga, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Engineering Industrial & Manufacturing Engineering

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Konstruksi Kandidat Gen AV1 Begomovirus pada pBI121 dan Introduksinya ke dalam Tembakau Menggunakan Vektor Agrobacterium tumefaciens Tri Joko Santoso; Muhammad Herman; Sri H Hidayat; Hajrial Aswidinnoor; Sudarsono Sudarsono
Jurnal AgroBiogen Vol 7, No 1 (2011): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v7n1.2011.p9-18

Abstract

Construction of Begomovirus AV1 Gene Candidate intopBI121 and Its Introduction into Tobacco by usingAgrobacterium tumefaciens Vector. Tri J. Santoso,Muhammad Herman, Sri H. Hidayat, HajrialAswidinnoor, and Sudarsono. Infection of Begomovirushas caused leaf curl disease in tomato. This infection hassignificantly impact on yield losses of tomato production.Recently, in Indonesia there was no effectively way tocontrol this disease. The use of resistant tomato variety isone of strategies to control this virus. Genetic engineeringtechnology gives an opportunity to develop the transgenictomato resistant to Begomovirus through pathogen derivedresistance (PDR) approach. The objectives of this studywere to construct the Begomovirus AV1 candidate gene inthe pBI121 and to introduce the construct into tobacco plantgenome through Agrobacterium tumefaciens vector. A seriesactivites in gene construct have been conducted includePCR amplification of AV1 gene using a pair of specificprimer, cloning the gene into pGEM-T easy, transformation ofthe clone into Escherichia coli DH5α competent cell,construct the gene into pBI121, and transform the constructinto A. tumefaciens. Leaf segments of in vitro tobacco plantwere transformed by co-cultivation with A. tumefacienscontaining ToLCV-AV1 construct. In the research activitiy,Indonesian Begomovirus AV1 gene was successfullyamplified and inserted in expression vector plasmid pBI121.Tobacco transformants carrying kanamycin-resistant gene(nptII gene) were regenerated and established in theglasshouse. Those transformant plants are expectedcontaining the AV1 gene.
Identitas dan Keragaman Genetik Begomovirus yang Berasosiasi dengan Penyakit Keriting pada Tomat Berdasarkan Teknik Polymerase Chain Reaction (PCR)- Restriction Fragment Length Polymorphism (RFLP) Tri Joko Santoso; Sri H. Hidayat; M. Herman; H. Aswidinnoor; Sudarsono Sudarsono
Jurnal AgroBiogen Vol 4, No 1 (2008): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v4n1.2008.p9-17

Abstract

Begomoviruses, members of the Geminivirus,are considered as emerging plant viruses. This was due tothe increasing incidences and severities of the diseases in anumber of economically important crops, including tomato.Genetic diversities of the Begomovirus isolates infectingtomato (Lycopersicon esculentum) of several areas in Indonesiawere analyzed by using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP)technique. A 1500 base pairs of PCR fragments amplified byusing degenerate primers for Begomovirus was digestedusing four restriction enzymes, i.e., DraI, EcoRI, RsaI, andPstI. The pattern of RE digested fragments of 8 Begomovirusisolates and the predicted RFLP fragments of the Begomovirusisolates in the GeneBank database were used to determinethe genetic identities and diversities among the isolates.Positive results of the PCR amplifications proved thatdiseased tomato plant samples collected from 8 locations inJava and Sumatra were infected with at least one Begomovirusisolate. The PCR amplification products, which weredigested using the four restriction enzymes indicated thepresence of polimorfisms among the DNA fragments of theBegomovirus isolates. Identifications of the Begomovirusindicated that the Brastagi, Bogor, Sragen, Ketep, and Boyolaliisolates were Tomato Leaf Curl Virus (ToLCV); theisolates from Malang and Blitar isolates were AgeratumYellow Vein Virus (AYVV), while one isolate from Kaliurangwas Tomato Yellow Leaf Curl Virus (TYLCV). Results of thephylogenetic analysis of the 8 Begomovirus isolates basedon Begomoviruses from the DNA database indicated thatthey belonged to three different groups.
Regenerasi Pepaya melalui Kultur In Vitro Diani Damayanti; Sudarsono Sudarsono; Ika Mariska; M. Herman
Jurnal AgroBiogen Vol 3, No 2 (2007): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v3n2.2007.p49-54

Abstract

A study was conducted in the Indonesian Center forAgricultural Biotechnology and Genetic Resources Researchand Development to optimize papaya regeneration systemsthrough in vitro culture. Four steps were done, i.e., callusinduction, callus regeneration, root formation, and acclimatization.Explant materials used were immature embryos ofpapaya cv. Burung. Immature papaya embryos were culturedon different media. The best medium for embryogeniccallus development was ½ MS + 10 mg/l 2.4-D + 60% sucrose+ 143 mg/l adenine sulphate + 50 mg/l myo inositol +400 mg/l glutamine, while that for callus embryo regenerationwas MS + 0.5 mg/l GA3 + 0.1 mg/l kinetin + Morel andWetmore Vitamin. Using this medium, the average of shootformation was three shoots per explant of embriogeniccallus, and the percentage of regenerated callus was 80%.The color of shoot derived from this treatment was green.Eighty percent of plants formed a complete root developmentusing ½ MS + 0.5 mg/l paclobutrazol media. Media hullof rice and compost was the best medium for papaya plantacclimatization. The percentage of survival on that acclimatizationstep was 65%.
Introduksi Konstruk Over-Ekspresi Kandidat Gen OsWRKY76 melalui Agrobacterium tumefaciens pada Tanaman Padi Nipponbare Aniversari Apriana; Atmitri Sisharmini; Wening Enggarini; Sudarsono Sudarsono; Nurul Khumaida; Kurniawan Rudi Trijatmiko
Jurnal AgroBiogen Vol 7, No 1 (2011): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v7n1.2011.p19-27

Abstract

Delivering of Over-Expression Construct OsWRKY76Candidate Gene in Rice cv. Nipponbare throughAgrobacterium tumefaciens. Aniversari Apriana, AtmitriSisharmini, Wening Enggarini, Sudarsono, Nurul.Khumaida, and Kurniawan R. Trijatmiko. Plant geneticimprovement can be done through classical breeding orgenetic engineering. WRKY is a transcription factor involvedin regulating plant defense responses. OsWRKY76 gene islocated in a narrow segment of chromosome 9 which isidentified previously to be related to wide spectrumresistance in rice. A sequence of OsWRKY76 (+1.200 bp)has available in the gene bank and it makes possible toisolate, clone, and construct the gene into over-expressionvector. The aim of this research was to assemble an overexpressionconstruct of OsWRKY76 candidate gene andintroduce it into rice through Agrobacterium-mediatedtransformation. A construct of pCAMBIA-1301::35S::OsWRKY76 has been successfully assembled andtransformed into embryogenic calli of rice cv. Nipponbareusing A. tumefaciens strain Agl-1 and EHA 105. A number of126 independent lines has been produced, in which Agl-1showed 3.8 times more efficient than EHA 105. PCR analysisof randomly selected 25 independent lines showed that allof them positively contained hptII gene, a selectable markerused in the over-expression construct of the OsWRKY76candidate gene. Based on the result, it could be concludedthat the over-expression construct of OsWRKY76 candidategene have been successfully introduced into the tissue ofNipponbare.
TOLERANSI TEMBAKAU TRANSGENIK YANG MENGEKSPRESIKAN GEN P5CS TERHADAP STRES KEKERINGAN Ahmad Riduan; Hajrial Aswidinnoor; Sudarsono ;; Djoko Santoso; Endrizal ;
Jurnal Pengkajian dan Pengembangan Teknologi Pertanian Vol 13, No 2 (2010): Juli 2010
Publisher : Balai Besar Pengkajian dan Pengembangan Teknologi Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpptp.v13n2.2010.p%p

Abstract

Tolerance of Transgenic Tobacco Expressing P5CS Gene Against Drought Stress. Drought is majorosmotic stress that dramatically limit plant growth and productivity. Proline accumulation has been correlatedwith tolerance to drought stress in plants. Therefore, overproduction of proline in plants may lead to increasedtolerance against these abiotic stresses. The objectives of this experiment were to determine the effects of droughtstress at the period of 15 – 90 days after planting (DAP) on growth of T1 plants derived from transgenic GStobacco, to evaluate their tolerance against drought stress, and to determine their leaf proline content. One groupof the tobacco plants were grown in plastic pots and subjected to stress condition during the period of 15 – 90DAP. The other group was grown optimally in plastic pot up to harvest period. All tobacco plants were harvestedat 91 DAP. Leaf proline content was determined at 63 DAP (after six periods of stress). The results indicatedreduced plant height, shoot diameter, leaf number, leaf dry weight and leaf area of all tobacco plants. Stresssensitivity index calculated using leaf dry weight character grouped T1 plants derived from P5CS transgenicGS tobacco into tolerance, medium tolerance and sensitive against drought stress while that of non-transgenicGS tobacco were only medium tolerance and sensitive against drought stress. Higher leaf proline content underdrought stress was observed in all T1 plants derived from P5CS transgenic tobacco than that of non-transgenicGS tobacco. These data demonstrated that proline accumulation as an osmoprotectant and that over-expressionP5CS gene results in the increased tolerance to osmotic stress in T1 plants derived from P5CS transgenic tobacco.Key words: Proline biosynthesis, proline accumulation, sensitivity index, biomass yield Stres kekeringan merupakan masalah utama stres osmotik yang dapat menjadi faktor pembataspertumbuhan dan produktivitas tanaman. Akumulasi prolina berkorelasi dengan tingkat toleransi tanamanterhadap stres kekeringan. Oleh karena itu over-produksi prolina diduga dapat meningkatkan toleransi tanamanterhadap stres kekeringan. Tujuan penelitian ini adalah (1) untuk mengevaluasi pengaruh stres kekeringanmelalui pengurangan air terhadap pertumbuhan dan hasil tanaman TI zuriat dari tembakau GS transgenik P5CSgenerasi TO, (2) menganalisis akumulasi prolina daun tanaman TI zurlat dari tembakau GS transgenik P5CSgenerasi TO kondisi stres dan non-stres, serta (3) menganalisis hubungan antara akumulasi prolina daun padakondisi stres kekeringan dengan pertumbuhan dan hasil tanaman. Percobaan dua faktor (tembakau transgenikstres kekeringan) disusun dengan rancangan acak kelompok. Sebagian tanaman yang diuji disiram setiap hari hingga mencapai kondisi kapasitas lapang dari awal tanam sampai dengan 90 HST dan digunakan sebagaiperlakuan non-stres. Sedangkan kelompok tanaman yang lain dipelihara dalam kondisi kapasitas lapanghingga 14 HST dan diberi perlakuan stres kekeringan dari umur 15 HST hingga panen (90 HST). Kandunganprolina diukur pada umur 63 HST (setelah 6x periode stres). Hasil penelitian menunjukkan semua tanamantembakau yang diuji mempunyai tinggi tanaman, diameter batang, jumlah, berat kering dan luas daun yanglebih rendah akibat perlakuan stres kekeringan yang diberikan dibandingkan dengan kondisi non-stres.Berdasarkan hasil perhitungan indeks sensitivitas terhadap stres kekeringan menggunakan peubah bobotdaun kering per tanaman maka tanaman TI zurlat dari tembakau GS transgenik P5CS generasi TO yang diujibersegregasi untuk kategori toleran, medium toleran dan peka, sedangkan tembakau GS non-transgenikdikategorikan sebagai medium toleran dan peka terhadap stres kekeringan. Tanaman TI zurlat dari tembakau GStransgenik P5CS generasi TO menunjukkan kandungan prolina yang lebih tinggi dalam kondisi stres kekeringandibandingkan dengan tembakau GS non-transgenik. Peningkatan akumulasi prolina yang cukup tinggi akibatover-ekspresi dari gen P5CS diduga berkorelasi dengan peningkatan toleransi tanaman terhadap stres kekeringan.Kata kunci : Biosintesis prolina, akumulasi prolina, indeks sensitivitas terhadap stres, produksi biomasa
KERAGAMAN GENETIKA VARIAN ABAKA YANG DIINDUKSI DENGAN ETHYLMETHANE SULPHONATE (EMS) RULLY DYAH PURWATI; SUDJINDRO SUDJINDRO; ENDANG KARTINI; SUDARSONO SUDARSONO
Jurnal Penelitian Tanaman Industri Vol 14, No 1 (2008): Maret 2008
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v14n1.2008.16-24

Abstract

ABSTRAKMutasi in vitro dengan perlakuan mutagen dapat digunakan untukmeningkatkan keragaman genetika abaka melalui keragaman somaklonal.Penelitian yang dilakukan bertujuan untuk: (1) menentukan konsentrasioptimum EMS untuk induksi keragaman somaklonal dalam kultur kalusembriogen abaka, (2) meregenerasikan bibit abaka varian dari kalusembriogen yang diberi perlakuan EMS, dan (3) mengevaluasi tipe danfrekuensi keragaman karakter kualitatif dan kuantitatif di antara populasitanaman mutan abaka yang diperoleh dari regenerasi kalus embriogenyang diberi perlakuan EMS. Penelitian dilakukan mulai bulan Agustus2003 sampai Agustus 2006 di Laboratorium Kultur Jaringan dan KebunPercobaan Karangploso, Malang pada Balai Penelitian TanamanTembakau dan Serat, Malang (Balittas). Hasil penelitian menunjukkanbahwa 0,6% EMS merupakan konsentrasi optimum karena padakonsentrasi tersebut diperoleh keragaman somaklonal paling banyak.Varian yang diperoleh menunjukkan karakter kualitatif dan kuantitatifabnormal. Tipe varian tersebut umumnya bersifat negatif dan kurangmenguntungkan dibandingkan dengan populasi standar. Tipe dan frekuensikeragaman kualitatif dan kuantitatif pada klon Tangongon berbeda denganklon Sangihe-1, mengindikasikan adanya pengaruh genotipe terhadapkeragaman somaklonal. Varian dari abaka klon Tangongon denganproduksi serat tertinggi (161,0 g dan 154,0 g/tanaman) diperoleh dariperlakuan EMS 0,3% (T1 28.1.1 dan T1 11.2.2), sedangkan dari klonSangihe-1, hasil serat tertinggi (35,0 g dan 40,0 g/tanaman) diperoleh dariperlakuan EMS 0,6% (S4 28.1.0 dan S4 56.2.0). Produktivitas tersebutlebih rendah dibandingkan dengan produksi serat tanaman kontrol klonTangongon (193,0 g/tanaman) dan Sangihe-1 (70 g/tanaman).Kata kunci : Abaka, Musa textilis, keragaman somaklonal, EMS, muta-genesis, in vitro, hasil, Jawa TimurABSTRACTGenetic Variability of Abaca Variants Induced byEthylmethane Sulphonate (EMS)In vitro mutation with mutagen treatment can be used to increasethe genetic variability of abaca by inducing somaclonal variation. Theobjectives of the experiments were to (1) determine optimum concen-tration of EMS to induce abaca somaclonal variation, (2) produce abacalines from EMS treated embryogenic calli and evaluate their performancein the field, and (3) evaluate type and frequency of qualitative andquantitative variant characters among regenerated abaca lines. Thisexperiment was conducted in Tissue Culture Laboratory and KarangplosoExperiment Station of Indonesian Tobacco and Fibre Crops ResearchInstitute (ItoFCRI) Malang from August 2003 to August 2006. The resultsshowed that EMS treatment on abaca embryogenic calli induced variation,and the optimum EMS concentration was 0.6%. The variants exhibited anumber of abnormal qualitative and quantitative characters which weregenerally negative characters since they showed lower value as comparedto control population. The presence of different types of qualitative andquantitative variant characters was genotype dependent. Variants fromabaca clone Tangongon having the highest fibre yield (161.0 g and 154.0g/plant) were obtained from 0.3% EMS treatment (T1 28.1.1 and T1 11.2.2variants). While variants from abaca clone Sangihe-1 having the highestfibre yield (35.0 g and 40.0 g/plant) were obtained from 0.6% EMStreatment (S4 28.1.0 and S4 56.2.0 variants). The fibre yield of controlclones, Tangongon and Sangihe-1, were 193.0 g and 70 g/plant,respectively.Key words: Abaca, Musa textilis, induced mutation, somaclonalvariation, field evaluation, yield, East Java
Pendugaan Parameter Genetik Ketahanan Tanaman Kakao terhadap Penyakit Busuk Buah Rubiyo Rubiyo; Sudarsono Sudarsono
Jurnal Tanaman Industri dan Penyegar Vol 2, No 3 (2011): Buletin Riset Tanaman Rempah dan Aneka Tanaman Industri
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

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Abstract

Rapid method for cocoa breeding as an effort to produce high productivity and quality cocoa clones which are resistant to main disease infected by Phytophtora palmivora, pathogen causing black pod disease, needs to be investigated. For that reason, providing the information about various genetic parameters will really assist to solve the problems in cocoa cultivation and farming in Indonesia. One of the estimation methods of some genetic parameters which is eligible to be used is diallel crossing analysis. The research aimed at estimating genetic parameter of cocoa resistance to the disease caused by P. palmivora, using half dialel crossing. The cross used five cocoa clones as parental clones ( ICCRI 3, TSH 858, DR 1, ICS 13 and Sca 6). The clones represented selected clones resulted from resistance evaluation of previous research, with the resistance level from vulnerable to resistant. The number of genotypes in this research were 15, consisting of 10 F1, and 5 parental clones. Research took place from 2007 to 2008 in Experimental Plot of Kaliwining, Indonesian Coffee and Cocoa Research Center, Jember East Java. Seedlings from the crossing used for the research of every combination consisted of 20 seedlings replicated 3 times. Inoculum type used was mycellia, from selected inoculum in research I. Inoculation was done in leaf and to maintain the moisture (90%) it was covered by plastic. Observation was conducted 3 days after inoculation on the spot area caused by P. palmivora infection. The research indicated that there was no gene interaction in determining resistance to the disease caused by P. palmivora which mostly influenced by additive gene actions. Kd / kr was 1,3594 indicated that there were more dominant gene in parental. Heritability values in a broad and narrow sense belong to high group.
Analisis Keragaman Genetik 28 Nomor Koleksi Kakao (Theobroma cacao L.) Berdasarkan Marka SSR Ilham Nur ardhi Wicaksono; Rubiyo Rubiyo; Dewi Sukma; Sudarsono Sudarsono
Jurnal Tanaman Industri dan Penyegar Vol 4, No 1 (2017): Jurnal Tanaman Industri dan Penyegar
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v4n1.2017.p13-22

Abstract

Analisis keragaman genetik koleksi plasma nutfah kakao menggunakan marka molekuler mempunyai peranan penting dalam program perakitan klon unggul baru. Ketersediaan klon komersial dan klon unggul lokal meningkatkan peluang keberhasilan perakitan klon unggul baru sehingga analisis keragaman genetik materi tersebut perlu dilakukan. Tujuan penelitian adalah menganalisis keragaman genetik 28 nomor koleksi kakao berdasarkan marka SSR yang berguna dalam pemilihan tetua persilangan. Penelitian  dilakukan  di Laboratorium Terpadu Balai Penelitian Tanaman Industri dan Penyegar (Balittri), Sukabumi, dan Laboratorium Biologi Molekuler Tanaman (PMB), Fakultas Pertanian, Institut Pertanian Bogor, mulai bulan November 2015 sampai Mei 2016. Analisis keragaman genetik dilakukan pada 28 klon kakao yang terdiri dari 13 klon unggul lokal dan 15 klon komersial. Ekstraksi DNA dilakukan dengan menggunakan prosedur berbasis CTAB (cetyltrimethylammonium bromide). Selanjutnya, DNA diamplifikasi dengan teknik PCR (polymerase chain reaction) menggunakan 20 pasang primer SSR (simple sequence repeats). Hasil penelitian menunjukkan semua marka SSR yang digunakan bersifat polimorfik dengan rata-rata nilai PIC (polymorphism information content) cukup tinggi, yaitu 57%. Pohon filogenetik yang dianalisis menggunakan program DARwin (Dissimilarity Analysis and Representation for Windows) versi 6.05 terbagi menjadi 3 kelompok besar yang menempatkan klon unggul lokal dan klon komersial bersama-sama dalam tiap-tiap kelompok. Klon unggul lokal diduga mempunyai asal usul yang dekat dengan klon komersial yang sudah dibudidayakan di Indonesia. Selain itu,beberapa klon kakao berpotensi menjadi tetua persilangan karena mempunyai jarak genetik cukup jauh. Hasil penelitian menunjukkan bahwa marka SSR merupakan alat bantu cukup potensial untuk menentukan tetua persilangan yang diharapkan dapat meningkatkan peluang heterosis pada keturunannya.
Efektifitas Daya Hambat Minyak Nabati Terhadap Pertumbuhan Koloni Beberapa Patogen Terbawa Benih Cabai Secara In Vitro Syamsudin Syamsudin; Satriyas Ilyas; Diah Manohara; Sudarsono Sudarsono
Jurnal Agrista Vol 11, No 2 (2007): Volume 11 Nomor 2 Agustus 2007
Publisher : Fakultas Pertanian, Universitas Syiah Kuala

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Abstract

Effectiveness of In Vitro Inhibitor Effects of Botanical Pesticides on Growth of Some Pepper Seed Born Pathogens ColoniesABSTRACT. Recently, the use of bio-pesticides significantly increases to control some plant diseases due to the drawback of chemical pesticides to environment. Some experiment have been done to evaluate the effectiveness of some vegetable oil as bio-pesticides to inhibit the colony growth of pepper seed born pathogens in vitro. Various concentrations of vegetable oil were added onto potato dextrose agar medium (PDA), afterward, the growth of pathogen colony in treatment was compared to control (medium without vegetable oil). Evaluation was done in certain period time depend on the growth of every pathogen colony Among the vegetable soil tested, clove oil and Andropogon nardus oil showed a very high inhibitory effect to colony growth of P. capsici, C. capsici, F. oxyporum, R solani, and S. rolfsii. Concentration 0.01% of those oil already inhibited the colony growth of all pathogens more than 75%, with the exception of A. nardus oil which required 0.03% to inhibit C. capsici colony. Concentration of clove and A. nardus oil above 0.01% completely inhibited growth of all pathogens colonies, except for F. oxyporum. Meanwhile, others vegetable oil like Curcuma xanthorrhiza, Kaempferia galangal, Kaempferia pandurata, and Curcuma domestica oil have a low inhibitory effect on growth of all pathogens colonies. To get inhibitory effect more than 75% on the growth of colonies, those vegetable oil needed concentration higher than 0.02%. However, K. galangal and C. domestica required 0.02% oil concentration for P. capsici and S. rolfsii, respectively.
Pengaruh Cekaman Kekeringan Terhadap Pertumbuhan, Hasil dan Kandungan Prolina Daun Cabai Yusniwati Yusniwati; Sudarsono Sudarsono; Hajrial Aswidinnoor; Sri Hendrastuti; Djoko Santoso
Jurnal Agrista Vol 12, No 1 (2008): Volume 12 Nomor 1 April 2008
Publisher : Fakultas Pertanian, Universitas Syiah Kuala

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Abstract

Drought Stress Effect on Growth, Yield and Leaf Proline Accumulation of Hot PepperABSTRACT. The objective of this experiment were to evaluate effects of drought stress at vegetative stage on growth, yield and leaf proline content of hot pepper. Drought stress was conditioned by reduction watering of crop show wilt symptom 70% during the period of 21 – 54 days after planting (DAP). Result of the experiment indicated that drought stress reduced plant height, branch number, stem, diameter, root length, shoot, root, and biomass dry weight and fruit yield. Sensivity index calculated based on biomass of five hot pepper cultivars showed that Prabu, laris, dan jati laba were the medium tolerance to drought stress. There was no drought tolerance cultivar if the sensivity index was calculated based on fruit yield.
Co-Authors AHMAD RIDUAN Aniversari Apriana Anwar, Aswaldi Atmitri Sisharmini Diah Manohara Diani Damayanti Djoko Santoso Djoko Santoso ENDANG KARTINI Endrizal ; Freta Kirana Balladona H. Aswidinnoor HAJRIAL ASWIDINNOOR Hasriadi Mat Akin Ika Mariska Ilham Nur Ardhi Wicaksono Ismail Maskromo Kurniawan Rudi Trijatmiko M. Herman Meynarti Sari Dewi Ibrahim Muhammad Herman Nurul Khumaida Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo RULLY DYAH PURWATI Satriyas Ilyas Sholeh Avivi Sri H Hidayat Sri H. Hidayat Sri Hendrastuti SUDJINDRO SUDJINDRO Sukma, Dewi Syafaruddin Syafaruddin Syamsudin Syamsudin Tri Joko Santoso Tri Joko Santoso Wening Enggarini Yusniwati Yusniwati