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Ika Mariska
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111
Agriculture, Biological Sciences & Forestry

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Regenerasi Pepaya melalui Kultur In Vitro Diani Damayanti; Sudarsono Sudarsono; Ika Mariska; M. Herman
Jurnal AgroBiogen Vol 3, No 2 (2007): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v3n2.2007.p49-54

Abstract

A study was conducted in the Indonesian Center forAgricultural Biotechnology and Genetic Resources Researchand Development to optimize papaya regeneration systemsthrough in vitro culture. Four steps were done, i.e., callusinduction, callus regeneration, root formation, and acclimatization.Explant materials used were immature embryos ofpapaya cv. Burung. Immature papaya embryos were culturedon different media. The best medium for embryogeniccallus development was ½ MS + 10 mg/l 2.4-D + 60% sucrose+ 143 mg/l adenine sulphate + 50 mg/l myo inositol +400 mg/l glutamine, while that for callus embryo regenerationwas MS + 0.5 mg/l GA3 + 0.1 mg/l kinetin + Morel andWetmore Vitamin. Using this medium, the average of shootformation was three shoots per explant of embriogeniccallus, and the percentage of regenerated callus was 80%.The color of shoot derived from this treatment was green.Eighty percent of plants formed a complete root developmentusing ½ MS + 0.5 mg/l paclobutrazol media. Media hullof rice and compost was the best medium for papaya plantacclimatization. The percentage of survival on that acclimatizationstep was 65%.
Pengujian Nomor-nomor Harapan Padi Tahan Al dan pH Rendah Hasil Seleksi In Vitro dengan Kultur Hara Ragapadmi Purnamaningsih; Ika Mariska
Jurnal AgroBiogen Vol 4, No 1 (2008): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v4n1.2008.p18-23

Abstract

Rice productivity in acid soil is very low because of low pH,low availability of N, P, K, Ca, Mg, Mo, toxicity of Al and Mn.Development of Al tolerant varieties could increase riceproductivity in acid soil. Somaclonal variation and in vitroselection method can be used to develop new Al tolerancevarieties. A rapid screening method is needed to select alarge number of new genotypes or new inbred lines in plantbreeding, such as solution culture methods to evalu-ate Altolerantrice. This methods was used to know the responseto Al in the seedling stage, root development, and pHchanging. In this experiment solution culture method wasused to evaluate the new genotypes derived from somaclonalvariation and in vitro selection methods. These newgenotypes have been tested the tolerance characteristic byusing AlCl36H2O at 6 concentrations (0, 100, 200, 300, 400,and 500 ppm). Yoshida solution with two Al concentrationwere used to tested these genotypes. Measurement of Altolerance was based on root development by using RelativeRoot Length (RRL), the relativity of root length at 45 ppm and0 ppm. Almost all of the genotypes have RRLs higher than0.7, which means that there was a positive correlationbetween the in vitro method and solution culture method. Inthis experiment pH changes were not applicable to measurethe tolerance of the rice genotypes to Al and low pH.
Co-Authors Diani Damayanti M. Herman Ragapadmi Purnamaningsih Sudarsono Sudarsono