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All Journal Jurnal Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat Jurnal Penelitian Tanaman Industri (Littri) Buletin Penelitian Tanaman Rempah dan Obat
SITTI FATIMAH SYAHID
Balai Penelitian Tanaman Rempah dan Obat
Agriculture, Biological Sciences & Forestry Decision Sciences, Operations Research & Management Industrial & Manufacturing Engineering

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PENGARUH PENGENCERAN MEDIA DASAR TERHADAP PERTUMBUHAN KULTUR JAHE DALAM PENYIMPANAN SECARA IN VITRO SITTI FATIMAH SYAHID; NURLIANI BERMAWIE
Jurnal Penelitian Tanaman Industri Vol 5, No 4 (2000): Maret, 2000
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v5n4.2000.115-118

Abstract

Effect of dilution of basic medium on ginger growth during conservation in vitroBasic medium dilution could affect the growth of the culture. The effect of basic medium dilution was evaluated at the laboratory of Research Institute for Spices and Medicinal Crops from October 1998 to June 1999. Sterile shoots of ginger were used as ex plants. Basic medium dilution from 3/4 MS, 1/2 MS, 1/4 MS and MS normal (control) were used as treatments. The media were enriched with BA 3 mg/1 and Sucrose 6%. The treatments wee arranged in a completely randomized design with three eplications. Results showed that all the dilution treatments inhibited the grow of the culture during live months of conservation period, especially on 1/4 MS + BA 3 mg/1 + Sucrose 6% . On the media, the number of shoots wee 3.55. Based on cultue performance the best medium for conservation was 1/2 MS + BA 3 mg/1 + Sucrose 6%. All of the culture was able to growth in regeneration media after they were conserved for ive months. Results of this experiment could be applied in reducing sub-cultue frequency to a new media and maintain the culture stability. e
PENGARUH PUPUK DAN JARAK TANAM TERHADAP PERTUMBUHAN DAN PRODUKSI JAHE ASAL KULTUR JARINGAN HOBIR HOBIR; SITTI FATIMAH SYAHID; IKA MARISKA
Jurnal Penelitian Tanaman Industri Vol 4, No 4 (1998): November, 1998
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v4n4.1998.129-134

Abstract

An alternative to produce healthy planting materials of ginger is through tissue cultue. However, the information on productivity and cultural practices or such mateial is still limited. Among the cultural aspects, fertilizer application and plant spacing mostly afect plant growth and yield. The objective of this study, was o evaluate the effect of fertilizer application and plant spacing on the growth and yield of rhizome. Planting materials (tissue culture and conventionally derived rhizomes) wee espectively planted in two plant spacings (60 cm x 40 cm and 40 cm x 30 cm) and applied with two levels of ertilizers (with and without fertilizer). The fertilizers applied were 40 tons stable manure and urea, TSP, and KCI wee 400 kg/ha. The expeiment was conducted in the Sukamulya Expeimental Station (Sukabumi)) rom August 1994 to  December 1995 in a split-split plot design in four replicates. Die main plot was ertilizer application, the sub plot was plant spacing and the sub-sub plot was planting material. The parameters used for evaluating the response of the plants to the treatments wee growth components (plant height stem circumeence, number of tillers and leaf size), and yield components (weight and size of rhizome). Results revealed that plants rom tissue culture generally showed the lower growth performances with lower yield and size of rhizomes, except in the number of tillers. The application of urea, TSP, and KCI at a respective rate of 400 kg/ha significantly increased plant height (rom 49 o 60 cm), stem circumerence (rom 2.2 o 2.6 cm) and weight of rhizome/clump (rom 206 o 379 g). Plant spacing did not affect any parameter observed. Interaction efect was shown between the source of planting materials and fertilizer application on the number of tillers and the thickness of rhizomes. For the plants rom tissue cultue ertilizer application significantly increase the number of tillers (rom 18 o 30 tillers/clump) and for those rom conventional rhizome the treatment did not afect the parameter. On the other hand, for the plants rom conventional rhizome ertilizer application improved the thickness of rhizome (rom 2.51 o 3.17 cm) while for those rom tissue cultue the teatment did not give any efect on the parameter. The thickness of rhizome was also afected by the interaction between fertilizer application and plant spacing. For the plant spacing of 40 cm x 30 cm, ertilizer application inceased the thickness of rhizome (from 1.68 to 2.11), while those of 60 cm x 40 cm, the teatment did not afected the parameter.
ADAPTASI DELAPAN NOMOR HARAPAN KUNYIT (Curcuma domestica Vahl.) TOLERAN NAUNGAN Sitti Fatimah Syahid; Cheppy Syukur; Nathalini Nova Kristina; Joko Pitono
Buletin Penelitian Tanaman Rempah dan Obat Vol 23, No 2 (2012): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v23n2.2012.%p

Abstract

Turmeric cultivation by farmer is mainly conducted under shading, so it needed turmeric superior seeds especially for shading cultivation. The aim of the research was to analiysis the adaptability based on yield and quality stability of turmeric promising lines under shading on three different agroecology Adaptation test for releasing the superior variety of crops was conducted at three locations of turmeric central production in Bringin 464 m asl, Nogosari, 425 m asl, and Simo 484 m asl. Tested was done in 2008 until 2010 under famer’s teak plantations. Eight promising lines of turmeric out of 70 accessions selected under artificial shading season in year of 2007/2008 at Cicurug, Sukabumi (West Java) and one local number were used as materials to be tested. The experiment was arranged in Randomized Block Design with three replications. Each block consisted of 48 plants. The observed parameters were growth component, yield, and curcumin content. Combined varian analysis result showed that there was no interaction effect between genotype and environment on the yield and curcumin content. However, all of the tested genotypes were signicifantly differed for their curcumin content. Turmeric promising line Cudo 04, with rhizome yield 0f 7.4 ton ha-1, had the highest curcumin (7.05%) and essential oil content (4.77%), and tolerant to the leaf spot disease. Therefore, this line is recommended to be released as a superior variety tolerant to shading. Based on its curcumin content, this line was also found as a superior one to be developed by herbal medicine industry.
PENGARUH AUKSIN IBA DAN NAA TERHADAP INDUKSI PERAKARAN INGGU SITTI FATIMAH SYAHID; NATALINI NOVA KRISTINA
Jurnal Penelitian Tanaman Industri Vol 20, No 3 (2014): September 2014
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v20n3.2014.122-129

Abstract

ABSTRAK Inggu (Ruta graveolens L.) merupakan salah satu tanaman obat langka di Indonesia yang perlu dilestarikan. Upaya konservasi tanaman inggu telah dilakukan secara in vitro di laboratorium Balittro selama 17 tahun pada kultur tunas. Untuk mengobservasi kestabilan genetik perlu dilakukan induksi perakaran. Penelitian ini bertujuan untuk mengetahui pengaruh auksin IBA dan NAA terhadap induksi perakaran inggu secara in vitro. Bahan tanaman yang digunakan adalah tunas steril inggu in vitro yang telah berumur 17 tahun, yang ditanam pada media dasar Murashige dan Skoog (MS) setengah konsentrasi (½ MS) yang diperkaya vitamin dari group B. Perlakuan yang diuji adalah beberapa taraf konsentrasi auksin IBA dan NAA (0; 0,001; 0,002; dan 0,003 mg/l). Rancangan yang digunakan adalah Rancangan Acak Lengkap dengan lima ulangan. Setiap ulangan terdiri dari lima botol yang berisi dua tanaman. Parameter yang diamati adalah jumlah, panjang, dan bentuk akar, serta jumlah tunas dan penampilan kultur. Hasil penelitian menunjukkan bahwa penggunaan media ½ MS yang diperkaya NAA pada konsentrasi rendah 0,001 mg/l menghasilkan jumlah akar terbanyak, yaitu 13,6 akar. Perlakuan ini juga menghasilkan banyak bulu-bulu akar yang menandakan akar yang sehat. Kata kunci: Ruta graveolens L., IBA, NAA, induksi perakaran, in vitro  The Effect of Auxin IBA and NAA to In Vitro Rooting Induction of Roe (Ruta graveolens L.)  ABSTRACTRoe (Ruta graveolens L.) is one of the Indonesian rare medicinal plants. An attempt to conserve roe, has been conducted through in vitro culture of sterile shoots at the laboratory of the Indonesian Spice and Medicinal Crops Research Institut (ISMCRI) for 17 years. To observe the genetic stability of culture following in vitro conservation for a long period, the collection must be tested in greenhouse and field. Therefore, it is necessary to induce rooting. The aim of the experiment was to observe the effect of IBA and NAA auxin to root induction of roe. The sterile shoots were used as material. They were planted on half-concentration (½ MS) on Murashige and Skoog (MS) medium, enriched with vitamin from group B. The experiment was arranged in a completely randomized design with five replications. Each replication consist of five bottles with two plants. The treatment tested were several concentrations of IBA and NAA (0; 0.001; 0.002; and 0.003 mg/l). The parameters observed were number, lenght, shape, and length of roots, and also the number of shoots and culture performance. The result showed that the use of ½ MS + NAA0.001 mg/l produced the highest number of roots  (13.6 roots). This treatment also produced a lot of root hairs which indicates a healthy roots. Key words: Ruta graveolens L., IBA, NAA, roots induction, in vitro
PENGARUH KOLKHISIN TERHADAP PENAMPILAN LADA P p er nigrum L.) MUTAN DAN ANALISIS PLOIDI NATALINI NOVA KRISTINA; SITTI FATIMAH SYAHID
Jurnal Penelitian Tanaman Industri Vol 21, No 3 (2015): September 2015
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v21n3.2015.125-130

Abstract

ABSTRAKKeragaman genetik plasma nutfah lada (Piper nigrum) di Indonesia rendah  sehingga  perlu  dilakukan  peningkatan  keragaman.  Penelitian dilakukan di Rumah Kaca Balittro Bogor mulai Januari 2012 sampai Juni 2013. Tujuan penelitian adalah untuk meningkatkan keragaman genetik lada varietas Petaling 1 menggunakan mutagen kimia kolkhisin. Biji lada direndam dalam larutan kolkhisin konsentrasi 0; 0,01; 0,03; dan 0,05% selama 4 jam dan disemai pada bak pasir. Masing-masing perlakuan diulang 3 kali  dan  setiap ulangan  terdiri atas 300 biji.  Pengamatan dilakukan  terhadap  persentase  perkecambahan  dan  fenotipe  tanaman, persentase tumbuh, tinggi tanaman, serta jumlah ruas dan daun pada umur dua bulan. Selanjutnya, sebanyak 20 individu dari total benih yang tumbuh dipilih berdasarkan rata rata penggabungan dari tanaman terpendek dan tertinggi. Individu terpilih diamati tinggi tanaman serta jumlah ruas dan daun pada umur empat bulan. Untuk melihat ragam genetik dilakukan analisis kandungan DNA dengan flowcytometry. Hasil penelitian menunjukkan bahwa perlakuan kolkhisin 0,01 dan 0,05% menghasilkan persentase perkecambahan benih di persemaian lebih tinggi. Pada lada mutan  vegetatif generasi  ke-0,  perubahan  morfologi  terindikasi  pada konsentrasi 0,03%.  Pada  generasi  mutan hasil  perbanyakan/turunan vegetatif pertama  perubahan  morfologi  pada  tanaman terjadi  pada perlakuan 0,05%. Namun, tidak ada perbedaan yang nyata pada tingkat ploidi lada pada semua perlakuan termasuk kontrol.Kata kunci:  Piper nigrum L., ragam genetik, mutan, kolkhisin, fenotip ABSTRACTEffect of Colchicine on the Phenothypic Performance of Pepper (Piper nigrum L.) Mutant and Ploidy analysisGenetic variability of pepper (Piper nigrum) in Indonesia was low, so it was needed to increase its variability. Research was conducted at the green house of Indonesian Spices and Medicinal Crops Research Institute, Bogor from January 2012 to June 2013. The aim of the research was to increase the genetic variability of pepper (Petaling 1) using chemical mutagen colchicine. Seeds of pepper were soaked in colchicine solution with several concentration (0; 0,01; 0,03; and 0,05%) for four hours, and then germinated on sand media. Every treatment consisted of 300 seeds and replicated three times. The parameter observed were germination percentage, plant phenotype, growth percentage, plant hight, number of node and leaves two months after planting. Further, from total seedling growth, 20 individual were selected based on average combined from highest and shortest plant. The selected individual observed their plant height, number of node and leaves on four months. Flowcytometri analysis from  the  selected  seedling  was  conducted to  find  interplant  genetic variabilities. The result showed that application of colchicin 0,01 and 0,05% performed the fast germination on the nursery compared with control, but no significant differencet on the growth parameters. In the mutant generation 0, the changes on morphology showed on 0,03% and at the first vegetative generation, the changes were indicated in plants from  0,05%  of  colchicine  treatment.  Flowcytometri  analysis  showed  no  significant differences on ploidi level of all treatments including control.Keywords:   Piper   nigrum,   genetic   variability,   mutant,  cholchicin, phenotype
PENGARUH PENGENCERAN MEDIA DASAR TERHADAP PERTUMBUHAN KULTUR JAHE DALAM PENYIMPANAN SECARA IN VITRO SITTI FATIMAH SYAHID; NURLIANI BERMAWIE
Jurnal Penelitian Tanaman Industri Vol 5, No 4 (2000): Maret, 2000
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v5n4.2000.115-118

Abstract

Effect of dilution of basic medium on ginger growth during conservation in vitroBasic medium dilution could affect the growth of the culture. The effect of basic medium dilution was evaluated at the laboratory of Research Institute for Spices and Medicinal Crops from October 1998 to June 1999. Sterile shoots of ginger were used as ex plants. Basic medium dilution from 3/4 MS, 1/2 MS, 1/4 MS and MS normal (control) were used as treatments. The media were enriched with BA 3 mg/1 and Sucrose 6%. The treatments wee arranged in a completely randomized design with three eplications. Results showed that all the dilution treatments inhibited the grow of the culture during live months of conservation period, especially on 1/4 MS + BA 3 mg/1 + Sucrose 6% . On the media, the number of shoots wee 3.55. Based on cultue performance the best medium for conservation was 1/2 MS + BA 3 mg/1 + Sucrose 6%. All of the culture was able to growth in regeneration media after they were conserved for ive months. Results of this experiment could be applied in reducing sub-cultue frequency to a new media and maintain the culture stability. e
PENGARUH PUPUK DAN JARAK TANAM TERHADAP PERTUMBUHAN DAN PRODUKSI JAHE ASAL KULTUR JARINGAN HOBIR HOBIR; SITTI FATIMAH SYAHID; IKA MARISKA
Jurnal Penelitian Tanaman Industri Vol 4, No 4 (1998): November, 1998
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v4n4.1998.129-134

Abstract

An alternative to produce healthy planting materials of ginger is through tissue cultue. However, the information on productivity and cultural practices or such mateial is still limited. Among the cultural aspects, fertilizer application and plant spacing mostly afect plant growth and yield. The objective of this study, was o evaluate the effect of fertilizer application and plant spacing on the growth and yield of rhizome. Planting materials (tissue culture and conventionally derived rhizomes) wee espectively planted in two plant spacings (60 cm x 40 cm and 40 cm x 30 cm) and applied with two levels of ertilizers (with and without fertilizer). The fertilizers applied were 40 tons stable manure and urea, TSP, and KCI wee 400 kg/ha. The expeiment was conducted in the Sukamulya Expeimental Station (Sukabumi)) rom August 1994 to  December 1995 in a split-split plot design in four replicates. Die main plot was ertilizer application, the sub plot was plant spacing and the sub-sub plot was planting material. The parameters used for evaluating the response of the plants to the treatments wee growth components (plant height stem circumeence, number of tillers and leaf size), and yield components (weight and size of rhizome). Results revealed that plants rom tissue culture generally showed the lower growth performances with lower yield and size of rhizomes, except in the number of tillers. The application of urea, TSP, and KCI at a respective rate of 400 kg/ha significantly increased plant height (rom 49 o 60 cm), stem circumerence (rom 2.2 o 2.6 cm) and weight of rhizome/clump (rom 206 o 379 g). Plant spacing did not affect any parameter observed. Interaction efect was shown between the source of planting materials and fertilizer application on the number of tillers and the thickness of rhizomes. For the plants rom tissue cultue ertilizer application significantly increase the number of tillers (rom 18 o 30 tillers/clump) and for those rom conventional rhizome the treatment did not afect the parameter. On the other hand, for the plants rom conventional rhizome ertilizer application improved the thickness of rhizome (rom 2.51 o 3.17 cm) while for those rom tissue cultue the teatment did not give any efect on the parameter. The thickness of rhizome was also afected by the interaction between fertilizer application and plant spacing. For the plant spacing of 40 cm x 30 cm, ertilizer application inceased the thickness of rhizome (from 1.68 to 2.11), while those of 60 cm x 40 cm, the teatment did not afected the parameter.
PENGARUH AUKSIN IBA DAN NAA TERHADAP INDUKSI PERAKARAN INGGU SITTI FATIMAH SYAHID; NATALINI NOVA KRISTINA
Jurnal Penelitian Tanaman Industri Vol 20, No 3 (2014): September 2014
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v20n3.2014.122-129

Abstract

ABSTRAK Inggu (Ruta graveolens L.) merupakan salah satu tanaman obat langka di Indonesia yang perlu dilestarikan. Upaya konservasi tanaman inggu telah dilakukan secara in vitro di laboratorium Balittro selama 17 tahun pada kultur tunas. Untuk mengobservasi kestabilan genetik perlu dilakukan induksi perakaran. Penelitian ini bertujuan untuk mengetahui pengaruh auksin IBA dan NAA terhadap induksi perakaran inggu secara in vitro. Bahan tanaman yang digunakan adalah tunas steril inggu in vitro yang telah berumur 17 tahun, yang ditanam pada media dasar Murashige dan Skoog (MS) setengah konsentrasi (½ MS) yang diperkaya vitamin dari group B. Perlakuan yang diuji adalah beberapa taraf konsentrasi auksin IBA dan NAA (0; 0,001; 0,002; dan 0,003 mg/l). Rancangan yang digunakan adalah Rancangan Acak Lengkap dengan lima ulangan. Setiap ulangan terdiri dari lima botol yang berisi dua tanaman. Parameter yang diamati adalah jumlah, panjang, dan bentuk akar, serta jumlah tunas dan penampilan kultur. Hasil penelitian menunjukkan bahwa penggunaan media ½ MS yang diperkaya NAA pada konsentrasi rendah 0,001 mg/l menghasilkan jumlah akar terbanyak, yaitu 13,6 akar. Perlakuan ini juga menghasilkan banyak bulu-bulu akar yang menandakan akar yang sehat. Kata kunci: Ruta graveolens L., IBA, NAA, induksi perakaran, in vitro  The Effect of Auxin IBA and NAA to In Vitro Rooting Induction of Roe (Ruta graveolens L.)  ABSTRACTRoe (Ruta graveolens L.) is one of the Indonesian rare medicinal plants. An attempt to conserve roe, has been conducted through in vitro culture of sterile shoots at the laboratory of the Indonesian Spice and Medicinal Crops Research Institut (ISMCRI) for 17 years. To observe the genetic stability of culture following in vitro conservation for a long period, the collection must be tested in greenhouse and field. Therefore, it is necessary to induce rooting. The aim of the experiment was to observe the effect of IBA and NAA auxin to root induction of roe. The sterile shoots were used as material. They were planted on half-concentration (½ MS) on Murashige and Skoog (MS) medium, enriched with vitamin from group B. The experiment was arranged in a completely randomized design with five replications. Each replication consist of five bottles with two plants. The treatment tested were several concentrations of IBA and NAA (0; 0.001; 0.002; and 0.003 mg/l). The parameters observed were number, lenght, shape, and length of roots, and also the number of shoots and culture performance. The result showed that the use of ½ MS + NAA0.001 mg/l produced the highest number of roots  (13.6 roots). This treatment also produced a lot of root hairs which indicates a healthy roots. Key words: Ruta graveolens L., IBA, NAA, roots induction, in vitro
PENGARUH KOLKHISIN TERHADAP PENAMPILAN LADA P p er nigrum L.) MUTAN DAN ANALISIS PLOIDI NATALINI NOVA KRISTINA; SITTI FATIMAH SYAHID
Jurnal Penelitian Tanaman Industri Vol 21, No 3 (2015): September 2015
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v21n3.2015.125-130

Abstract

ABSTRAKKeragaman genetik plasma nutfah lada (Piper nigrum) di Indonesia rendah  sehingga  perlu  dilakukan  peningkatan  keragaman.  Penelitian dilakukan di Rumah Kaca Balittro Bogor mulai Januari 2012 sampai Juni 2013. Tujuan penelitian adalah untuk meningkatkan keragaman genetik lada varietas Petaling 1 menggunakan mutagen kimia kolkhisin. Biji lada direndam dalam larutan kolkhisin konsentrasi 0; 0,01; 0,03; dan 0,05% selama 4 jam dan disemai pada bak pasir. Masing-masing perlakuan diulang 3 kali  dan  setiap ulangan  terdiri atas 300 biji.  Pengamatan dilakukan  terhadap  persentase  perkecambahan  dan  fenotipe  tanaman, persentase tumbuh, tinggi tanaman, serta jumlah ruas dan daun pada umur dua bulan. Selanjutnya, sebanyak 20 individu dari total benih yang tumbuh dipilih berdasarkan rata rata penggabungan dari tanaman terpendek dan tertinggi. Individu terpilih diamati tinggi tanaman serta jumlah ruas dan daun pada umur empat bulan. Untuk melihat ragam genetik dilakukan analisis kandungan DNA dengan flowcytometry. Hasil penelitian menunjukkan bahwa perlakuan kolkhisin 0,01 dan 0,05% menghasilkan persentase perkecambahan benih di persemaian lebih tinggi. Pada lada mutan  vegetatif generasi  ke-0,  perubahan  morfologi  terindikasi  pada konsentrasi 0,03%.  Pada  generasi  mutan hasil  perbanyakan/turunan vegetatif pertama  perubahan  morfologi  pada  tanaman terjadi  pada perlakuan 0,05%. Namun, tidak ada perbedaan yang nyata pada tingkat ploidi lada pada semua perlakuan termasuk kontrol.Kata kunci:  Piper nigrum L., ragam genetik, mutan, kolkhisin, fenotip ABSTRACTEffect of Colchicine on the Phenothypic Performance of Pepper (Piper nigrum L.) Mutant and Ploidy analysisGenetic variability of pepper (Piper nigrum) in Indonesia was low, so it was needed to increase its variability. Research was conducted at the green house of Indonesian Spices and Medicinal Crops Research Institute, Bogor from January 2012 to June 2013. The aim of the research was to increase the genetic variability of pepper (Petaling 1) using chemical mutagen colchicine. Seeds of pepper were soaked in colchicine solution with several concentration (0; 0,01; 0,03; and 0,05%) for four hours, and then germinated on sand media. Every treatment consisted of 300 seeds and replicated three times. The parameter observed were germination percentage, plant phenotype, growth percentage, plant hight, number of node and leaves two months after planting. Further, from total seedling growth, 20 individual were selected based on average combined from highest and shortest plant. The selected individual observed their plant height, number of node and leaves on four months. Flowcytometri analysis from  the  selected  seedling  was  conducted to  find  interplant  genetic variabilities. The result showed that application of colchicin 0,01 and 0,05% performed the fast germination on the nursery compared with control, but no significant differencet on the growth parameters. In the mutant generation 0, the changes on morphology showed on 0,03% and at the first vegetative generation, the changes were indicated in plants from  0,05%  of  colchicine  treatment.  Flowcytometri  analysis  showed  no  significant differences on ploidi level of all treatments including control.Keywords:   Piper   nigrum,   genetic   variability,   mutant,  cholchicin, phenotype
ADAPTASI DELAPAN NOMOR HARAPAN KUNYIT (Curcuma domestica Vahl.) TOLERAN NAUNGAN Sitti Fatimah Syahid; Cheppy Syukur; Nathalini Nova Kristina; Joko Pitono
Buletin Penelitian Tanaman Rempah dan Obat Vol 23, No 2 (2012): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v23n2.2012.%p

Abstract

Turmeric cultivation by farmer is mainly conducted under shading, so it needed turmeric superior seeds especially for shading cultivation. The aim of the research was to analiysis the adaptability based on yield and quality stability of turmeric promising lines under shading on three different agroecology Adaptation test for releasing the superior variety of crops was conducted at three locations of turmeric central production in Bringin 464 m asl, Nogosari, 425 m asl, and Simo 484 m asl. Tested was done in 2008 until 2010 under famer’s teak plantations. Eight promising lines of turmeric out of 70 accessions selected under artificial shading season in year of 2007/2008 at Cicurug, Sukabumi (West Java) and one local number were used as materials to be tested. The experiment was arranged in Randomized Block Design with three replications. Each block consisted of 48 plants. The observed parameters were growth component, yield, and curcumin content. Combined varian analysis result showed that there was no interaction effect between genotype and environment on the yield and curcumin content. However, all of the tested genotypes were signicifantly differed for their curcumin content. Turmeric promising line Cudo 04, with rhizome yield 0f 7.4 ton ha-1, had the highest curcumin (7.05%) and essential oil content (4.77%), and tolerant to the leaf spot disease. Therefore, this line is recommended to be released as a superior variety tolerant to shading. Based on its curcumin content, this line was also found as a superior one to be developed by herbal medicine industry.
Co-Authors Cheppy Syukur HOBIR HOBIR IKA MARISKA Joko Pitono NATALINI NOVA KRISTINA Natalini Nova Kristina Nathalini Nova Kristina Nurliani Bermawie