Garuda - Garba Rujukan Digital

DAYA HAMBAT EKSTRAK KENCUR (Kaempferia galanga L.) TERHADAP Trichophyton mentagrophytes DAN Cryptococcus neoformans JAMUR PENYEBAB PENYAKIT KURAP PADA KULIT DAN PENYAKIT PARU Djaenudin Gholib
Buletin Penelitian Tanaman Rempah dan Obat Vol 20, No 1 (2009): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v20n1.2009.%p

Penelitian ini bertujuan untuk menge-tahui adanya efek daya hambat ekstrak etanol rimpang kencur (Kaempferia galanga L.) ter-hadap Trichophyton mentagrophytes, yaitu jamur jenis kapang penyebab penyakit kurap pada kulit, dan Cryptococcus neoformans, jamur jenis ragi penyebab penyakit paru pada manusia atau hewan. Penelitian dilakukan di laboratorium Mikologi Balai Besar Penelitian Veteriner (BBALITVET) Bogor mulai Feb-ruari sampai April 2008. Ekstrak etanol rim-pang kencur yang digunakan diekstraksi di Balai Penelitian Tanaman Obat dan Aromatik (BALITTRO), Bogor. Pengujian dilakukan dengan uji in vitro dengan metode tuang (pouring dilution method). Ekstrak kencur diencerkan pada taraf 0,03; 0,06; 0,09; 0,12; dan 0,15% untuk diuji daya hambatnya ter-hadap T. mentagrophytes, dan 0,25; 0,50; 1,0; 1,5; dan 2% untuk uji daya hambat terhadap C. neoformans. Masing-masing 1 ml ekstrak dan jamur uji yang dilarutkan dalam air suling steril (enceran 10-3) dituangkan ke dalam cawan petri steril, lalu dicampur secara merata. Kemudian media agar Sabouraud yang dicairkan, dituang-kan ke masing-masing cawan petri. Setelah membeku, biakan diinkubasi pada suhu 37o C selama 5 hari. Pengujian dilakukan dengan 3 kali ulangan. Pengamatan dilakukan terhadap pertumbuhan koloni jamur uji, dan dihitung jumlahnya. Pada enceran ekstrak yang menunjukkan tidak adanya pertumbuhan koloni jamur, ditentukan sebagai nilai konsentrasi hambat minimal (KHM). Hasil penelitian menunjukkan nilai KHM ekstrak adalah 0,15% terhadap T. mentagrophytes, dan 2% terhadap C. neoformans. Daya hambat ekstrak rimpang kencur lebih besar terhadap T. mentagrophytes diban-dingkan dengan C. neoformans.

Cases of aspergillosis in Javan Hawk-eagle (Spizaetus bartelst): Isolation of the causative agents and the pathological changes of the diseas Sukardi Hastiono; Rini Damayanti; Djaenudin Gholib
Jurnal Ilmu Ternak dan Veteriner Vol 1, No 2 (1995)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Two cases of aspergillosis in Javan Hawk-eagle (Spizaetus banelsi) from Safari Garden of Indonesia, Cisarua, Bogor, have been reported . Specimens of lungs, airsacs, livers, spleens, kidneys, and intestines were examined mycologically and pathologically . Mycological examination revealed in the isolation of the causative agents from lungs and airsacs, ie . Aspergillus niger from one bird, and Aspergillusfianigarus from the other one . Pathological anatomy examination showed formation of yellowish white miliary nodule lesions, especially in the lungs and airsacs, while histopathological examination showed congestion and granulomatous tissue formation in the lungs, ie . specific lesion of mycotic infection with necrotic zone in the centre, surrounded by lymphocytes, macrophages, fibrin, and fungal hyphae . Pathological changes of the other organs were also described . These findings were the first officially description of aspergillosis cases in these carnivorous birds .

Development of serological technique for examination of aspergillosis in chicken Djaenudin Gholib
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 2 (2005): JUNE 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Aspergillosis detection by using serological method has not been reported in Indonesia. In this case, a study was conducted, by using mycelium extract of A. fumigatus as the antigen. Rabbits and chickens were injected with the antigen to produce positive serum (antiserum). The antigen and antiserum were tested serologicaly by Immunodiffusion/Agar Gel Precipitation (AGP), ELISA and Immunoblot. Chicken serum of broiler and layer collected from field were also included in the test. All positive serum of the experimentally animals gave positive results with all methods of serological tests. No bands of precipitation reaction in AGP test with chicken serum from the field. Both chicken and rabbit positive serum with ELISA test showed high Optical Density (OD), while field chicken serum from broiler commonly gave lower OD compared to layer. Immunoblot test of chicken positive serum showed bands of reaction with the antigen in nitrocellulose membrane, approximately on 33, 38, 44, 52, 70, 77, 97, and 110 kDa, meanwhile field chicken serum with high OD in ELISA test, showed bands approximately on 16, 18, 33, 38, 44, 47, 52, 70, 77, 84, 97, and 110 kDa. It means that the field chicken serum contain immunoglobulin molecules has spesific antibody of aspergillus antigen. It is concluded that the ELISA test can be used for screening on chicken aspergillosis in serological methode. Key Words: Antigen, Aspergillus fumigatus, Serology, Chicken

The preparation of Dermatophilus congolensis antigen and its testing by means of immunodiffusion test and electrophoresis Djaenudin Gholib; Subiyanto .
Jurnal Ilmu Ternak dan Veteriner Vol 3, No 3 (1998)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The filtrate antigen ofDermatophilus congolensis was prepared based on the Makinde method, whereas the whole cell antigen was based on the Bida and Kelley method. Filtrate antigen of Dermatophilus congolensis has been tested with positive serum from experimental animals, guinea pigs and sheep by means of immurrodiffitsion test and electrophoresis . Positive serum was produced by inoculation of whole cell antigen of D. congolensis to the animals . The results showed that the immunodiffusion test resulted in one and two precipitation lines with positive serum of sheep and guinea pigs respectively. Electrophoresis SDS-PAGE presented about 8 bands with molecular weight in the range from above 30 kD to more than 94 kD. The bands were then transferred into nitrocellulose membrane and gave positive reaction with positive serum from sheep. Key words : Antigen, Dermatophilus congolensis, immunodiffusion, electrophoresis

Dermatophytes Fungi Trichophyton Verrucosum A Causative Agent of Ringworm Disease on Cattle Djaenudin Gholib; S Rachmawati
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 20, No 1 (2010): MARCH 2010
Publisher : Indonesian Center for Animal Research and Development

Ringworm is a superficial mycosis which affected surface parts of the body such as skin, hair, nail or horn. These parts are rich of keratine that required for the fungi to grow. The disease affects both animals and human (zoonosis), and it results in hair loss, crusted of skin, swelling, erythema and itchy. The disease is considered as important because it affects health condition and animal production. The causative agent is fungal dermatophytes group especially Trichophyton verrucosum. Cases occurred in Indonesia and first officially reported and published in 1980 on imported dairy cows from Australia, and a causative agent was identified as Trichophyton verrucosum. Recently, the same cases occurred in young dairy calves and lactating cattle. Pathogenity test in rabbits by artificial infection revealed the growth of the colonies. Post infection and vaccination provide resistency on cattle. Therefore beside therapeutic method, the vaccination program is useful to be included in prevention of the disease. Key words: Trichophyton verrucosum, ringworm, cattle

DAYA HAMBAT EKSTRAK KENCUR (Kaempferia galanga L.) TERHADAP Trichophyton mentagrophytes DAN Cryptococcus neoformans JAMUR PENYEBAB PENYAKIT KURAP PADA KULIT DAN PENYAKIT PARU Djaenudin Gholib
Buletin Penelitian Tanaman Rempah dan Obat Vol 20, No 1 (2009): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v20n1.2009.%p

Penelitian ini bertujuan untuk menge-tahui adanya efek daya hambat ekstrak etanol rimpang kencur (Kaempferia galanga L.) ter-hadap Trichophyton mentagrophytes, yaitu jamur jenis kapang penyebab penyakit kurap pada kulit, dan Cryptococcus neoformans, jamur jenis ragi penyebab penyakit paru pada manusia atau hewan. Penelitian dilakukan di laboratorium Mikologi Balai Besar Penelitian Veteriner (BBALITVET) Bogor mulai Feb-ruari sampai April 2008. Ekstrak etanol rim-pang kencur yang digunakan diekstraksi di Balai Penelitian Tanaman Obat dan Aromatik (BALITTRO), Bogor. Pengujian dilakukan dengan uji in vitro dengan metode tuang (pouring dilution method). Ekstrak kencur diencerkan pada taraf 0,03; 0,06; 0,09; 0,12; dan 0,15% untuk diuji daya hambatnya ter-hadap T. mentagrophytes, dan 0,25; 0,50; 1,0; 1,5; dan 2% untuk uji daya hambat terhadap C. neoformans. Masing-masing 1 ml ekstrak dan jamur uji yang dilarutkan dalam air suling steril (enceran 10-3) dituangkan ke dalam cawan petri steril, lalu dicampur secara merata. Kemudian media agar Sabouraud yang dicairkan, dituang-kan ke masing-masing cawan petri. Setelah membeku, biakan diinkubasi pada suhu 37o C selama 5 hari. Pengujian dilakukan dengan 3 kali ulangan. Pengamatan dilakukan terhadap pertumbuhan koloni jamur uji, dan dihitung jumlahnya. Pada enceran ekstrak yang menunjukkan tidak adanya pertumbuhan koloni jamur, ditentukan sebagai nilai konsentrasi hambat minimal (KHM). Hasil penelitian menunjukkan nilai KHM ekstrak adalah 0,15% terhadap T. mentagrophytes, dan 2% terhadap C. neoformans. Daya hambat ekstrak rimpang kencur lebih besar terhadap T. mentagrophytes diban-dingkan dengan C. neoformans.

5. Important Mycotic Diseases In Animal Livestock Caused By Some Agriculture Fungi In Indonesia Riza Zainuddin Ahmad; Djaenudin Gholib
The International Journal of Tropical Veterinary and Biomedical Research Vol 1, No 1 (2016): Vol. 1 (1) May 2016
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

One of contribution for Indonesian state from Agriculture sector is livestock animals. This livestock animals in Indonesia is divided to large and small ruminant, poultry, and other commercial animals. All these animals can be infected by some agriculture fungi which causes mycosis. The disease cause economic loss by morbidity and mortality. In chicken (poultry), aspergillosis caused by Aspergillus sp can be very important among mycotic diseases. In ruminant ringworm (dermatofitosis) caused by dermatofit fungi which infected skin, and mastitis caused by fungi in Dairy cattle causes decrease milk production. Histoplasmosis caused by fungi such as Histoplasma farciminosum is called Selakarang, infect horse. This paper discribes these mycotic diseases and control and to give more information of fungal disease caused by some agriculture fungi.

Title

Found 7 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 7 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 7 Documents
Search