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All Journal Jurnal Veteriner Buletin Penelitian Tanaman Rempah dan Obat Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Buletin Penelitian Tanaman Rempah dan Obat The International Journal of Tropical Veterinary and Biomedical Research

Djaenudin Gholib

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Author-ID : 2705215

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Cases of aspergillosis in Javan Hawk-eagle (Spizaetus bartelst): Isolation of the causative agents and the pathological changes of the diseas Sukardi Hastiono; Rini Damayanti; Djaenudin Gholib
Jurnal Ilmu Ternak dan Veteriner Vol 1, No 2 (1995)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Two cases of aspergillosis in Javan Hawk-eagle (Spizaetus banelsi) from Safari Garden of Indonesia, Cisarua, Bogor, have been reported . Specimens of lungs, airsacs, livers, spleens, kidneys, and intestines were examined mycologically and pathologically . Mycological examination revealed in the isolation of the causative agents from lungs and airsacs, ie . Aspergillus niger from one bird, and Aspergillusfianigarus from the other one . Pathological anatomy examination showed formation of yellowish white miliary nodule lesions, especially in the lungs and airsacs, while histopathological examination showed congestion and granulomatous tissue formation in the lungs, ie . specific lesion of mycotic infection with necrotic zone in the centre, surrounded by lymphocytes, macrophages, fibrin, and fungal hyphae . Pathological changes of the other organs were also described . These findings were the first officially description of aspergillosis cases in these carnivorous birds .

Development of serological technique for examination of aspergillosis in chicken Djaenudin Gholib
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 2 (2005): JUNE 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Aspergillosis detection by using serological method has not been reported in Indonesia. In this case, a study was conducted, by using mycelium extract of A. fumigatus as the antigen. Rabbits and chickens were injected with the antigen to produce positive serum (antiserum). The antigen and antiserum were tested serologicaly by Immunodiffusion/Agar Gel Precipitation (AGP), ELISA and Immunoblot. Chicken serum of broiler and layer collected from field were also included in the test. All positive serum of the experimentally animals gave positive results with all methods of serological tests. No bands of precipitation reaction in AGP test with chicken serum from the field. Both chicken and rabbit positive serum with ELISA test showed high Optical Density (OD), while field chicken serum from broiler commonly gave lower OD compared to layer. Immunoblot test of chicken positive serum showed bands of reaction with the antigen in nitrocellulose membrane, approximately on 33, 38, 44, 52, 70, 77, 97, and 110 kDa, meanwhile field chicken serum with high OD in ELISA test, showed bands approximately on 16, 18, 33, 38, 44, 47, 52, 70, 77, 84, 97, and 110 kDa. It means that the field chicken serum contain immunoglobulin molecules has spesific antibody of aspergillus antigen. It is concluded that the ELISA test can be used for screening on chicken aspergillosis in serological methode. Key Words: Antigen, Aspergillus fumigatus, Serology, Chicken

The preparation of Dermatophilus congolensis antigen and its testing by means of immunodiffusion test and electrophoresis Djaenudin Gholib; Subiyanto .
Jurnal Ilmu Ternak dan Veteriner Vol 3, No 3 (1998)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The filtrate antigen ofDermatophilus congolensis was prepared based on the Makinde method, whereas the whole cell antigen was based on the Bida and Kelley method. Filtrate antigen of Dermatophilus congolensis has been tested with positive serum from experimental animals, guinea pigs and sheep by means of immurrodiffitsion test and electrophoresis . Positive serum was produced by inoculation of whole cell antigen of D. congolensis to the animals . The results showed that the immunodiffusion test resulted in one and two precipitation lines with positive serum of sheep and guinea pigs respectively. Electrophoresis SDS-PAGE presented about 8 bands with molecular weight in the range from above 30 kD to more than 94 kD. The bands were then transferred into nitrocellulose membrane and gave positive reaction with positive serum from sheep. Key words : Antigen, Dermatophilus congolensis, immunodiffusion, electrophoresis

Co-Authors Riza Zainuddin Ahmad Riza Zainuddin Ahmad Riza Zainuddin Ahmad S Rachmawati Sembiring, Rinawati Subiyanto . Sukardi Hastiono

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Journal : Jurnal Ilmu Ternak Veteriner