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Journal : Jurnal Veteriner

Mastitis Mikotik Akibat Terinfeksi Candida spp dan Trichosporon spp pada Peternakan Sapi Perah di Bogor, Bandung, dan Jakarta (MYCOTIC MASTITIS CAUSED BY CANDIDA SPP AND TRICHOSPORON SPP ON DAIRY FARM IN BOGOR, BANDUNG, AND JAKARTA) Riza Zainuddin Ahmad; Djaenudin Gholib
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Mycotic mastitis is a disease affecting dairy cows and the causative agent is fungi of yeast, and alsomolds, but the disease is dominantly caused by yeast. In Indonesia reported of the disease is very rare.Generally, the mycotic mastitis is chronic causing great economic losses. This study aim was to show thatthere are still cases of mycotic mastitis in dairy cows in the area of Bandung, Bogor and Jakarta. Isolationand identification of fungi that caused mycotic mastitis were performed from the positive milk samplesteseted with California Mastitis Test (CMT). Of the 184 positive samples mastitis tested with CMT, 71samples mycotic mastitis were obtained, The 71 samples consisted of 13 molds and 84 yeasts based onthe isolates findings. Consecutive areas where commonly found mycotic mastitis were Bogor (50%);Bandung (38%); and Jakarta (27%). The number of positive samples in mycotic mastitis caused by moldinfection findings obtained less than yeasts. The yeast of Trichosporon spp found 34 isolates which washigher than Candida spp at 26 isolates. The existence of this yeast contamination was associated with thehygiene of the cage and the dairy cows. Control needs to be done to reduce the cases of mycotic mastitis.
Pemberian Duddingtonia flagrans dan Saccharomyces cerevisiae Meningkatkan Produksi Susu dan Menurunkan Populasi Cacing pada Sapi (THE TREATMENT OF DUDDINGTONIA FLAGRANS AND SACCHAROMYCES CEREVISIAE INCREASE MILK PRODUCTION AND DECREASE WORM POPULATION IN Riza Zainuddin Ahmad; Djaenudin Gholib
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Duddingtonia flagrans isolates are commonly used as biological control, and Saccharomyces cerevisiaeas probiotics. This study aimed to test isolates D. flagrans and S. cerevisiae as a biological agent andprobiotics. Tests carried out on beef cattle and dairy cows, which grouped into treatment (Administrationof D. flagrans and S. cerevisiae) and controls. For examination worm was also made in the grass. The studywas done in seven months The results of this study indicate that there is increase of 10 to 15% of the milkproduction in dairy cows after treatment (p> 0.05) but the beef was no difference between the treatmentand control. Treatment of D. flagrans significantly reduced the population of worms in the grass andanimals. Base on the result, it was concluded that D. flagrans and S. cerevisiae can be used together toincrease milk production and reduce the population of worms in cattle.
Cemaran Kapang pada Pakan Sapi dan Uji In Vitro Sirih terhadap Pertumbuhan Kapang Aspergillus flavus (MOLD CONTAMINATION IN CATTLE FEED AND IN VITRO ASSAY OF PIPER BETEL AGAINTS GROWTH OF MOLD CONTAMINANT ASPERGILLUS FLAVUS ) Riza Zainuddin Ahmad; Djaenudin Gholib
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (101.083 KB) | DOI: 10.19087/jveteriner.2017.18.3.453

Abstract

Contamination of mold in feed and Ingridients of feed is important because pathogenic and toxigenic mold will contaminate and cause mycotic and mycotoxicosis on livestock especially cattle. Information regarding the data is required in an attempt to controll of mold contaminant. Base on the previous study piper betel leaf (Piper betle) showed high activity as antimold. The aim of this study were to obtain data of mold contamination in cattle feed and ingredients of feed from the provinces of Banten, Lampung, Jakarta and West Java, and to test piper betel as an antimold herbal from traditional medicinal plants originated from Indonesia. Isolation and identification of fungi were conducted on the flour, glycerides, onggok, corn, peanut, coconut, coffee, concentrates, lamtoro, pineapple, rice, grass, palm, cassava, tofu lees, fish meal, bone meal from the provinces of Banten, Lampung, Jakarta and West Java. Isolation was done by plating the samples on agar medium, The mold have grown on media was identified. Feed that has been mixed with the extracts and powders plus mold inoculum was incubated. After 3=7 days incubation, colony forming unit (CFU) of the mixtures were counted. The results showed that the majority of feed contaminated with mold, but still below the threshold. The mold contamination in wheat flour, corn, concentrates and tofu lees exceeds from the threshold. Aspergillus sp, A. amstelodami, A. clavatus, A. Candidus, A. flavus, A. fumigatus, A. glaucus, A. niger, Cladosporium sp., Curvularia sp., Fusarium sp., Hyphomycetes sp., Mycelia sterilata, Mucor sp., Paecilomyces sp., Penicillium sp., and Rhizopus sp. Penicillium sp were most commonly found in the feed as much as 2.56 x 107 CFU. At a concentration of 10%. in vitro test showed that the piper betel leaf in powder form is more effective than extract form to inhibit the growth of A.flavus The conclusion of this study was flour, corn, concentrates and tofu lees contaminated by molds. Penicillium sp and 17 species of mold were the most frequently found compared to other fungi. Powders the best form of the piper betel as antimold. ABSTRAK Cemaran kapang pada pakan dan bahan penyusunnya adalah penting sebab kapang yang tergolong patogenik dan toksigenik dapat mencemari dan menyebabkan mikosis dan mikotoksikosis pada ternak sapi. Informasi mengenai kapang pencemar diperlukan dalam usaha pengendaliannya. Berdasarkan penelitian sebelumnya diketahui daun sirih (Piper betle) mempunyai aktivitas antikapang yang tinggi. Penelitian ini bertujuan untuk memperoleh data cemaran kapang pada pakan ternak sapi dan bahan penyusunnya dari propinsi Banten, Lampung, DKI Jakarta, dan Jawa Barat, serta menguji sirih sebagai obat herbal antikapang yang telah terpilih dari tanaman obat tradisional asli Indonesia. Isolasi dan identifikasi kapang telah dilakukan pada tepung, gliserida, onggok, jagung, kacang, kelapa, kopi, konsentrat, lamtoro, nenas, beras, rumput, sawit, singkong, ampas tahu, tepung ikan, tepung tulang dari provinsi Banten, Lampung, DKI Jakarta, dan Jawa Barat. Isolasi dilakukan dengan membiakkan sampel pada media agar, Kapang yang sudah tumbuh pada media diidentifikasi. Pakan yang telah dicampur dengan ektrak dan serbuk ditambahkan inokulum kapang, kemudian diinkubasi. Setelah 3-7 hari diinkubasi, dihitung colony forming unit (CFU) yang berkembang. Hasil penelitian menunjukkan bahwa sebagian besar pakan tercemar kapang, tetapi levelnya masih berada di bawah batas ambang. Cemaran kapang pada tepung, jagung, konsentrat, dan ampas tahu melebihi batas ambang. Kapang-kapang tersebut adalah Aspergillus sp, A. amstelodami, A. clavatus, A. candidus, A. flavus, A. fumigatus, A. glaucus, A. niger, Cladosporium sp., Curvularia sp., Fusarium sp., Hyphomycetes sp., Miselia sterilata, Mucor sp., Paecilomyces sp., Penicillium sp., dan Rhizopus sp. Kapang Penicillium sp adalah yang paling banyak ditemukan pada pakan yakni sebanyak 2,56.107 CFU. Uji in vitro menunjukkan bahwa daun sirih dalam bentuk serbuk lebih efektif dibandingkan bentuk ekstrak untuk menghambat pertumbuhan A. flavus pada konsentrasi 10%. Simpulan penelitian ini adalah tepung, jagung, konsentrat dan ampas tahu tercemar oleh kapang. Ditemukan 17 jenis kapang pencemar pakan dan kapang Penicillium sp yang paling banyak jumlahnya. Antikapang sirih yang terbaik adalah dalam bentuk serbuk.