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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Tanah dan Lingkungan (Journal of Soil Science and Environment) Jurnal Natur Indonesia
Lukman Gunarto
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Environmental Science

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Pengaruh CdCl2 terhadap Produksi Eksopolisakarida dan Daya Hidup Azotobacter Hindersah, Reginawanti; Arief, Dedeh Hudaya; Soemitro, Soetijoso; Gunarto, Lukman
Jurnal Natur Indonesia Vol 12, No 1 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (49.62 KB) | DOI: 10.31258/jnat.12.1.34-37

Abstract

The contamination of toxic heavy metal Cadmium (Cd) in soils will be endanger the human health because it ismore available comparing to another toxic heavy metals. One method of Cd-contaminated soil bioremediation isusing exopolysachharide-producing bacteria Azotobacter. Exopolysachharides (EPS) can mobilize Cd through theformation of complex Cd-EPS which sequentially can increase the availability of Cd for plants uptake. A laboratoryexperiment has been done to study the EPS production and the viability of six Azotobacter isolates in the liquidculture containing 0.01, 0.1, and 1 mM CdCl2. The bacteria were cultured in liquid medium with and without CdCl2 for72 hours at room temperature. The EPS production was determined by gravimetric method after precipitationusing acetone and centrifugation at 7000 rpm. The result was that all of Azotobacter isolates produce EPS in thepresence of CdCl2. In the culture with 1 mM CdCl2, the density of Azotobacter sp. isolate BS3, LK5, LKM6 increasedsignificantly, and that of isolate LH16 decreased. No significant effect of CdCl2 on the density of isolate BS2 andLH15. This research suggested that some Azotobacter isolates were relatively resistence to the Cd and could bedeveloped as biological agents in Cd-contaminated soil bioremediation.
AKTIVITAS ENZIM SELULASE MIKROBA YANG DIISOLASI DARI JERAMI PADI DI PERSAWAHAN PASANG SURUT DI KALIMANTAN SELATAN Fakhrur Razie; Iswandi Anas; Atang Sutandi; Lukman Gunarto; Sugiyanta Sugiyanta
Jurnal Ilmu Tanah dan Lingkungan Vol 13 No 2 (2011): Jurnal Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (254.775 KB) | DOI: 10.29244/jitl.13.2.43-48

Abstract

Enzim selulase terdiri dari tiga enzim ekstraselular yang bekerja secara sinergis dalam mendegredasi selulosa, yakni endoglukanase, eksoglukanase dan β-glukosidase. Tiga enzim tersebut berperan dalam mendegradasi selulosa menjadi gula sederhana. Penelitian ini bertujuan untuk mengisolasi dan menyeleksi mikroba (bakteri dan fungi) berdasarkan aktivitas enzim selulase dari mikroba tanah yang diisolasi dari persawahan pasang surut Kalimantan Selatan. Kemampuan mengekskresikan enzim endoglukanase dinilai berdasarkan nilai indeks selulolitik pada media CMC dan kemampuan mengekskresikan enzim eksoglukanase dan β-glukosidase diukur dari aktivitas kedua enzim tersebut menggunakan metode Mandel yang dimodifikasi. Hasil yang diperoleh menunjukkan bahwa kisaran nilai indeks selulolitik dari mikroba selulolitik di persawahan pasang surut tipe A sebesar 2.29-3.72, di lahan tipe B sebesar 2.66-5.41, dan di lahan tipe C sebesar 1.84-3.34. Aktivitas eksoglukanase dari mikroba selulolitik di persawahan pasang surut tipe A sebesar 0.27-1.65 nkat mL-1, lahan tipe B sebesar 0.37-1.85 nkat mL-1, dan lahan tipe C sebesar 0.31-1.85 nkat mL-1. Mikroba selulolitik dari persawahan pasang surut Kalimantan Selatan memiliki aktivitas β-glukosidase sebesar 0.05-1.52 nkat mL-1. Isolat- isolat mikroba selulolitik yang memiliki aktivitas selulase tertinggi adalah isolat bakteri selulolitik J11, J42, R23, BK12, C52, TB41, B82 dan SN123, dan isolat fungi selulolitik ST33, ST22, TB31, B52, GA22, TD11, PI52 dan P31.
Efisiensi Serapan Hara dan Hasil Padi pada Budidaya SRI di Persawahan Pasang Surut dengan Menggunakan Kompos Diperkaya Razie, Fakhrur; Anas, Iswandi; Sutandi, Atang; Sugiyanta, ,; Gunarto, Lukman
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 41 No. 2 (2013): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (418.378 KB) | DOI: 10.24831/jai.v41i2.7509

Abstract

There are two main problems when system of rice intensification (SRI) cultivation is carried out at tidal areas, e.g. the presence of Fe which is potentially toxic to plants and low nutrients availability. Utilization of Azotobacter-enriched compost is a choice to overcome iron toxicity as well a source of nutrients. The aims of this research were to study the effect of Azotobacter enriched compost to reduce the rates of inorganic fertilizers applied, to increase the efficiency of N, P, and K uptake, and to increase plant growth and yield. Two rice cultivation methods were applied namely SRI and conventional rice cultivation methods on tidal lands (rice fields) of South Kalimantan. A completely randomized block design with two factors was applied. The main plots were SRI and conventional rice cultivation, and as subplots were eight types of fertilizer that is a combination between Azotobacter-enriched compost with inorganic fertilizers rates. The results showed that soil nutrient availability, uptake and efficiency of N, P, and K with SRI technique was higher than conventional cultivation. Azotobacter- enriched compost on the tidal land (rice fields) could reduce the use of N and K fertilizer by 25%. Furthermore, the efficiency of N and P uptake in enriched compost with 75% of fertilizer N, P, and K were not different with enriched compost with 75% of fertilizer. Ciherang rice yields with SRI cultivation was approximately 22% higher compared to that from conventional rice cultivation. Keywords: Azotobacter-enriched compost, conventional rice cultivation, inorganic fertizers
Co-Authors Atang Sutandi Dedeh Hudaya Arief Fakhrur Razie Fakhrur Razie Iswandi Anas Iswandi Anas Luh Putu Ratna Sundari Reginawanti Hindersah Soetijoso Soemitro Sugiyanta