Dwinita W. Utami
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar No. 3A, Bogor 16111 Telp. (0251) 8337975; Faks. (0251) 8338820

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Keragaan Sifat Tahan Penyakit Blas dan Agronomi Populasi Silang Balik dan Haploid Ganda Turunan IR64 dan Oryza rufipogon Dwinita W. Utami; A. Dinar Ambarwati; Aniversari Apriana; Atmitri Sisharmini; Ida Hanarida; Sugiono Moeljopawiro
Buletin Plasma Nutfah Vol 16, No 2 (2010): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v16n2.2010.p90-95

Abstract

Blast Resistance Performance of Promising Lines Derived from Backcross and Double Haploid Population Between IR64 and Oryza rufipogon. Developing blast resistance varieties with superior agronomical performance has been the one of the important priorities in rice breeding program. Based on the purpose of this study the double haploid and backcross populations were developed using the most popular cultivar IR64 as recurrent parent and wild rice species Oryza rufipogon (Acc. IRGC 105491) as blast resistance donor parent. This study was initiated to analyze the blast resistance and agronomical performance of double haploid populations (DH_I, DH_II and DH_III) and backcross populations (BC2, BC3, and BC5), based on the green house and field screening tests. The results of statistical analysis showed that the blast resistance performance of DH population were diverse among DH_I, DH_II and DH_III. The smallest diversity was on the DH_III population. The same results were also detected on BC populations. The smallest diversity was on BC5 population. The diversity comparison between DH and BC population showed that DH_III population had smaller variation than BC5. Indicated that DH_III population has the most fixed population. The agronomic performance evaluation of DH_III population selected lines showed that Bio1, Bio2, and Bio8 qualitified as the candidate of promising lines. AbstrakPerakitan varietas tahan blas sebagai galur harapan, merupakan salah satu prioritas dalam program pemuliaan padi. Dalam rangka mendukung program tersebut, telah dilakukan pembentukan populasi haploid ganda (HG) dan silang balik (BC) dengan IR64 sebagai tetua berulang dan Oryza rufipogon (No. aksesi IRGC 105491) sebagai tetua donor gen tahan penyakit blas. Penelitian ini bertujuan menganalisis keragaan tingkat ketahanan galur-galur haploid ganda (HG_I, HG_II, dan HG_III) dan galur-galur silang balik (BC2, BC3, dan BC5) terhadap penyakit blas di rumah kaca dan lapang, sehingga diperoleh kandidat galur harapan. Hasil pengujian beberapa populasi HG dan BC menunjukan bahwa terdapat variasi keragaan yang berbeda-beda. Variasi paling kecil terdapat pada populasi HG_III. Hasil yang sama juga diperoleh pada populasi silang balik (BC2-BC5). Variasi paling kecil terdapat pada populasi BC5. Bila dibandingkan antar populasi HG dan BC, tingkat variasi pada populasi HG_III lebih kecil dibandingkan dengan tingkat variasi pada populasi BC5. Hal ini menunjukkan bahwa tingkat homosigositas paling tinggi terdapat pada populasi HG_III. Berdasarkan evaluasi penampilan agronomis beberapa galur HG_III terpilih, diperoleh tiga galur kandidat galur harapan Bio1, Bio2, dan Bio8.
Evaluasi Toleransi Plasma Nutfah Padi Lokal pada Lahan Masam Kahat Fosfor Tintin Suhartini; Dwinita W. Utami; Ida Hanarida
Buletin Plasma Nutfah Vol 19, No 1 (2013): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v19n1.2013.p%p

Abstract

Phosporus (P) deficiency is one oflimiting factor for rice growth. In Indonesia P deficiency muchoccurs in acid soils. The use of P deficiency tolerant varietiesis the best solution compared to the application of P fertilizerdue to more efficient in cost. The purpose of this study was toevaluate the local rice germplasm collected from severalregions in Indonesia to P deficiency in acid soil. The study wasconducted in Jasinga West Java during wet season of 2006-2007 with the soil condition lack of P. The experiments wereconducted with the two treatments: first, without P fertilizerand second with P fertilizer equivalent of 25 kg P/ha. The total100 accessions of rice germplasm were screened in this fieldbased on a randomized block design with three replications.Fertilizing were given on experiments I and II with thecomposition: urea 300 kg/ha and 100 kg KCl. The field designwas done by plotting size is 1 x 5 m2, spacing plant of 25 cm x25 cm, and planting two seeds per hole. The yield componentscharacters which were observed: number of tillers, plant dryweight, plant height and flowering. The results of combinedanalysis showed that there is a significant interaction betweenP and genotype on the tiller number, whereas the dry weight ofplant, plant height, and flowering were is not significant. Theevaluation of 100 local rice genotypes to P deficiency byindicators of the tiller number and dry weight of plantsobtained 19 genotypes that are tolerant to P deficiency with therelative value of tillers number and dry weight of plants morethan 80%. Local varieties Mandalet, Ganefo, Padi Belanda,Pulut Jangan, Padi Ubek Bala, and Padi Krayan were the mosttolerant to conditions without P which showed the tillersnumber more 1-21% rather than the conditions with Pfertilizer. There are three selected genotypes, Pulut Jangan,Padi Ubek Bala, and Padi Krayan which increased the numberof tillers and dry weight of plants in without P conditions. Theincreasing of tillers number were reach 1-17% while the dryweight increased 12-41%.