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All Journal Jurnal Tanaman Industri dan Penyegar
Nur Kholilatul Izzah
(Genetics and Molecular Breeding), (Scopus ID: 55584249900; H-index: 6), Balai Penelitian Tanaman Industri dan Penyegar
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Industrial & Manufacturing Engineering

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Keragaman Genetik Klon Kakao Lokal Sulawesi Tenggara Berdasarkan Marka SSR dan Karakter Morfologi Nur Kholilatul Izzah; Budi Martono; Baharuddin Baharuddin; Edi Wardiana
Jurnal Tanaman Industri dan Penyegar Vol 5, No 3 (2018): Jurnal Tanaman Industri dan Penyegar
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v5n3.2018.p95-104

Abstract

Molecular and morphological characterization of cacao clones obtained from exploration in Southeast Sulawesi is very important to know their superiority and genetic relationships. Analysis of genetic diversity using molecular markers is also useful for detecting duplication found among collected clones. The research aimed to determine the genetic diversity of local cacao clones derived from Southeast Sulawesi based on SSR markers and morphological characters. The research was conducted at Cacao Research Sub-Station, Lebojaya Village, Konda Subdistrict, South Konawe Regency, Southeast Sulawesi, and Integrated Laboratory of Indonesian Industrial and Beverage Crops Research Institute Sukabumi, and Molecular Biology Laboratory of Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development Bogor, from April to November 2015. Genetic diversity analysis was performed on 21 cacao clones covering 19 local clones and 2 national varieties using 22 SSR markers. The molecular characterization results showed that 11 markers are polymorphic, and subsequently used to group cacao clones using NTSYS program. The grouping results divided the cacao clones into 4 main groups at 0.46 genetic similarity values. Based on genetic distance values >0.7, 8 combinations of cacao clones can be selected as parental clones with the expectation to increase the effect of heterosis on progeny. On the other hand, result of morphological characterization generally indicated the diversity between the four cacao groups. Based on molecular and morphological characterization, it can be seen that cacao clones derived from Southeast Sulawesi have a high diversity and can be utilized in the development program of new improved varieties.
Analisis Daya Gabung dan Korelasi Karakter Jumlah Buah Kakao pada Persilangan Setengah Dialel Nur Kholilatul Izzah; Ilham Nur Ardhi Wicaksono; Cici Tresniawati; Edi Wardiana
Jurnal Tanaman Industri dan Penyegar Vol 6, No 3 (2019): Jurnal Tanaman Industri dan Penyegar
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v6n3.2019.p99-108

Abstract

Analysis of combining ability and correlation between characters in cacao plays an important role and benefits in an effort to obtain superior high-yield genetic material. This analysis makes it easy to find out the best combination of parents in producing superior progeny. The study aimed to analyze the combining ability and correlation of cacao pod numbers in F1 population generated from half-diallel crossing. The study was conducted at Pakuwon Experimental Station, Sukabumi, West Java, at an altitude of 450 m above sea level with Latosol soil type and B climate type (Schmidt and Fergusson), from 2014 until 2018. The experiment was arranged using a randomized block design (RBD) with 10 combinations of half-diallel crossing method that were repeated three times. The parental genotypes used were ICCRI 03, TSH 858, DR 1, ICS 13, and SCA 6. The variables observed were the number of healthy pods, number of rotten pods, number of cherelle wilts, and total pod numbers. The combining ability was analyzed using the Griffing 4 method, while correlation analysis was carried out phenotypically and genotypically.The results showed the action of non-additive genes in the F1 generation and three combinations of crosses, namely ICCRI 03 x TSH 858, TSH 858 x DR 1, and ICS 13 x SCA 6 had the opportunity to be developed into superior hybrids. In addition, it is known that there were phenotypic and genotypic positive correlations between the observed pod component characters.
Co-Authors Baharuddin Baharuddin Budi Martono Cici Tresniawati Edi Wardiana Ilham Nur Ardhi Wicaksono