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All Journal Jurnal Kesehatan Lingkungan (JKL) Jurnal Tumbuhan Obat Indonesia
Gusti Ayu Made Ratih Kusuma Ratna Dewi
Poltekkes Kemenkes Denpasar
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Medicine & Pharmacology Public Health

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IDENTIFIKASI CEMARAN Escherichia coli DAN FAKTOR PENCEMAR PADA DAGING AYAM DI PASAR KETAPIAN DENPASAR TIMUR Putu Indah Arta Pratiwi; Gusti Ayu Made Ratih Kusuma Ratna Dewi; I Gede Sudarmanto
Jurnal Kesehatan Lingkungan (JKL) Vol 12, No 1 (2022)
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/jkl.v12i1.2013

Abstract

Chicken meat has high protein and easily to be found in traditional markets. Chicken meat can be contamination by bacteria, especially by Escherichia coli. This research was to identify Escherichia coli contamination and contamination factors on chicken meat in East Denpasar Ketapian market. This research used a descriptive method with probability sampling technique, namely simple random sampling. Total samples used were 12 samples of chicken meat. The results show 6 samples (50 %) were positively contaminated by Escherichia coli. Contamination factors cause by environmental factors (garbage with heaped conditions, trash in open conditions, water not flow), equipment hygiene (not clean knives), and personal hygiene factors (sellers not clean their hands or use gloves and wear aprons in dirty conditions). Conclusion, 50 % of chicken meat samples from East Denpasar Ketapian market were contaminated by Escherichia coli and contamination factors that can affect are environmental factor, equipment hygiene and personal hygiene.
The Anticancer Potential of Ethanol and Aqueous Extracts of Pluchea indica L. Leaves Against the WiDr Colorectal Cancer Cell Line Nur Habibah; Ni Nyoman Astika Dewi; Luh Ade Wilan Krisna; Gusti Ayu Made Ratih
Jurnal Tumbuhan Obat Indonesia Vol. 18 No. 2 (2025): December 2025
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jtoi.v18i2.3061

Abstract

Colorectal cancer represents the second leading cause of cancer-related mortality worldwide and ranks asthe third most common cancer among both men and women. P. indica L. has demonstrated significantanticancer potential, attributed to its bioactive constituents, including phenolic and flavonoid compounds.Solvent type significantly influences extract quality and quantity, directly affecting pharmacological activity.This study aimed to evaluate the anticancer activity of ethanol and aqueous extracts from P. indica L. leavesin vitro, against the WiDr colorectal cancer cell line. Anticancer effects and IC50 values were determined bymeasuring cytotoxicity using the MTT assay. Results showed that both ethanol and aqueous extractsexhibited anticancer potential against the WiDr cell line. The ethanol extract exhibited higher cytotoxicity,as evidenced by reduced cancer cell viability (IC₅₀ of 21.82μg/mL±3.53), and was classified as active. Theaqueous extract had an IC₅₀ of 1,591.58μg/mL±272.8 and was classified as inactive. In conclusion, theethanol extract of P. indica L. showed greater potential for development as an anticancer agent againstcolorectal cancer. Keywords: Anticancer, Colorectal_cancer, Extract, Pluchea indica L., WiDr
Co-Authors I Gede Sudarmanto Luh Ade Wilan Krisna Ni Nyoman Astika Dewi Putu Indah Arta Pratiwi