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All Journal Bioscience Jurnal Pendidikan Biologi Indonesia BIOEDUKASI Jurnal SOLMA Inteligensi : Jurnal Ilmu Pendidikan
Endrik Nurrohman
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Mathematics Other

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Journal : Bioscience

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The number of trichoma leaves, preference of Bemisia tabaci, and resistance soybean genotype against cowpea mild motle virus after treatment variation doses of Nitrogen Endrik Nurrohman; Siti Zubaidah; Heru Kuswantoro
Bioscience Vol 6, No 1 (2022): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202261113785-0-00

Abstract

Bemisia tabaci is a dangerous insect for soybeans because it attacks the leaves and is a major obstacle in soybean cultivation and is considered a vector of Cowpea Mildmotle Virus (CpMMV). The part of the plant that defends itself from invading organisms is the trichome. Nitrogen nutrients can increase the number of trichomes in plant. This type of research is experimental research. The study design is a factorial design which is maintained in a complete group of randomized groups (RAKL). The first factor was a nitrogen dose consisting of four levels, such as N1 (0g), N2 (0.180g), N3 (0.36g), and N4 (0.543g), while the second factor was a genotype consisting of seven soybean genotypes UM.4-1, UM.7-2, UM.2-4, UM.7-6, UM.6-2, Wilis and Gumitir varieties. The results showed that there was a significant relationship between the number of upper trichomes and lower trichomes with the number of Bemisia tabaci. The interaction treatment had significant effect on the amount of upper surface of leaf tricoma and lower surface of leaf. Treatment of 0,543/ polybag nitrogen dose gave the most significant influence to the number of upper trichomes and lower trichomes. The results of correlation-regression analysis show that the number of lower trichomes contributes 65.7% in describing the number of Bemisia tabaci. The number of upper trichomes contributed 89.4% in describing the number of B. tabaci. Plants with a large number of trichomes and dense have low Bemisia tabaci preference and resistance to Cowpea Mild Motle Virus attacks in high level.Bemisia tabaci merupakan serangga berbahaya bagi kedelai karena menyerang daun dan menjadi kendala utama dalam budidaya kedelai dan dianggap sebagai vektor Cowpea Mildmotle Virus (CpMMV). Bagian tumbuhan yang mempertahankan diri dari serangan organisme pengganggu adalah trikoma. Unsur hara nitrogen dapat meningkatkan jumlah trikoma pada tanaman. Jenis penelitian ini adalah penelitian eksperimen. Desain penelitian adalah desain faktorial yang dipertahankan dalam kelompok lengkap kelompok acak (RAKL). Faktor pertama adalah dosis nitrogen yang terdiri dari empat taraf yaitu N1 (0g), N2 (0,180g), N3 (0,36g), dan N4 (0,543g), sedangkan faktor kedua adalah genotipe yang terdiri dari tujuh genotipe kedelai. Varietas UM.4-1, UM.7-2, UM.2-4, UM.7-6, UM.6-2, Wilis dan Gumitir. Hasil penelitian menunjukkan bahwa terdapat hubungan yang signifikan antara jumlah trikoma atas dan trikoma bawah dengan jumlah Bemisia tabaci. Perlakuan interaksi berpengaruh nyata terhadap jumlah permukaan atas daun trikoma dan permukaan bawah daun. Perlakuan dosis nitrogen 0,543/polybag memberikan pengaruh paling nyata terhadap jumlah trikoma atas dan trikoma bawah. Hasil analisis korelasi-regresi menunjukkan bahwa jumlah trikoma bawah berkontribusi 65,7% dalam menggambarkan jumlah Bemisia tabaci. Jumlah trikoma atas memberikan kontribusi 89,4% dalam menggambarkan jumlah B. tabaci. Tanaman dengan jumlah trikoma yang banyak dan lebat memiliki preferensi Bemisia tabaci yang rendah dan ketahanan terhadap serangan Cowpea Mild Motle Virus pada tingkat yang tinggi.
Stomata leaves characteristics of sapindaceae family in malabar forest, Malang city Endrik Nurrohman; Roimil Latifa; Samsun Hadi
Bioscience Vol 6, No 2 (2022): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202262118189-0-00

Abstract

Malabar forests are composed of various plant families, one of which is the Sapindaceae family. Differences in family, plant species, and environmental factors can affect the number of stomata and the size of plant cells. The purpose of this study was to explore the characteristics (number, density, type, shape, and size) of leaf stomata of plants of the Sapindaceae family in the Malabar forest of Malang City. This type of research is descriptive. The research was conducted in the Malabar City Forest of Malang City and the Biology Laboratory of the University of Muhammadiyah Malang. The study started from March 2021 to September 2021. The sampling technique was carried out using the cruising method. The method of collecting stomatal characteristics data using a light microscope and Scanning Electrone Microscope (SEM). The research data obtained were analyzed by descriptive statistics. The results showed that (1) there were 5 types of plants from the Sapindaceae family, there were variations in the size of the stomata parts of the Sapindaceae (2) family, the longest guard cell length was the stomata of the matoa leaf (22.4 m), the widest guard cell width was the matoa leaf (14.1 m). The longest pore length of rambutan leaves (15.8 m), and the widest pore width of longan leaf stomata (3.88 m), overall stomata are kidney-shaped, stomata types are anomocytic, paracytic, and cyclocytic, the highest stomata density kiara payung leaves (37.44 cells/mm2).Hutan malabar terdiri dari berbagai famili tumbuhan, salah satunya adalah famili Sapindaceae. Perbedaan famili, jenis tumbuhan, dan faktor lingkungan dapat mempengaruhi jumlah stomata dan ukuran sel tumbuhan. Tujuan penelitian ini adalah untuk mengetahui karakteristik (jumlah, kerapatan, jenis, bentuk, dan ukuran) stomata daun tumbuhan famili Sapindaceae di hutan Malabar Kota Malang. Jenis penelitian ini adalah deskriptif. Penelitian dilakukan di Hutan Kota Malabar Kota Malang dan Laboratorium Biologi Universitas Muhammadiyah Malang. Penelitian dimulai pada bulan Maret 2021 hingga September 2021. Teknik pengambilan sampel dilakukan dengan menggunakan metode cruising. Metode pengumpulan data karakteristik stomata menggunakan mikroskop cahaya dan Scanning Electrone Microscope (SEM). Data penelitian yang diperoleh dianalisis dengan statistik deskriptif. Hasil penelitian menunjukkan bahwa (1) terdapat 5 jenis tumbuhan dari famili Sapindaceae, terdapat variasi ukuran bagian stomata dari famili Sapindaceae (2) panjang sel penjaga yang paling panjang adalah stomata daun matoa (22,4 m), lebar sel penjaga terluas adalah daun matoa (14,1 m). Panjang pori terpanjang daun rambutan (15,8 m), dan lebar pori terlebar stomata daun kelengkeng (3,88 m), stomata keseluruhan berbentuk ginjal, tipe stomata anomositik, parasitik, dan siklositik, kerapatan stomata tertinggi daun kiara payung (37,44 sel/mm2).
Co-Authors Abdulkadir Rahardjanto Ainur Rofieq Eko Cahyono Eko Susetyarini Heru Kuswantoro Roimil Latifa Roimil Latifa Samsun Hadi Siti Zubaidah Sri Wahyuni