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Makrozoobentos Diversity in Various Substrates Type in Way Sindalapai River, Liwa Botanical Garden Rani Setiana; Tugiyono Tugiyono; Gregorius Nugroho Susanto; Suratman Suratman
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 7 No. 1 (2020)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v7i1.15

Abstract

The study of macrozoobenthos diversity in Way Sindalapai River can be used as one of the basic concepts in sustainable resource management in Liwa Botanical Garden area. Macrozoobenthos has an important role in the aquatic ecosystem, such as a food source for aquatic biota and as a detritivore. This study aims to determine the diversity of macrozoobenthos based on different sediment substrates in Way Sindalapai River, Liwa Botanical Garden, West Lampung. This study uses a purposive sampling method based on differences in the sediment substrates: muddy, sandy, rocky, and scrub with 3 replications. Sampling was carried out using a stratified filter and tweezers to separate the organism from the substrate. Samples were preserved in 70% alcohol and were identified by identification books at the Ecology Laboratory, FMIPA Universitas Lampung. The results of the study found 10 macrozoobenthos orders with a total of 155 individuals included in 13 families. The results of the calculation of diversity index values (H ') macrozoobenthos for all types of 2.26 (medium category). The highest macrozoobenthos dominance index (D) of the four substrates is in the rocky substrate that is 0.41 (low category). While the evenness index value (E) of macrozoobenthos in the Sindalapai Way River was 0.44 (medium category).
Determination of Aedes Spp. Mosquito Breeding Spots as a High-Risk Area Through Maya Index Analysis in Way Halim Bandar Lampung Athiyya Nurfadhilah; Emantis Rosa; M. Kanedi; Tugiyono Tugiyono
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 6 No. 2 (2019)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v6i2.36

Abstract

Dengue Haemorrhagic Fever (DHF) is a disease that is transmitted through the Aedes aegypti and Aedes albopictus mosquito vectors. The Maya index (MI) is an indicator to identify whether an area is at high risk as a breeding site for mosquitoes. This study aims to determine the types of breeding places and types of mosquito larvae of DHF and MI vectors in the Way Halim District of Bandar Lampung. The method used was a survey method on 100 houses in 5 villages in Way Halim District, namely Jaga Baya 1, Jaga Baya 2, Jaga Baya 3, Way Halim Permai, and Gunung Sulah. Data analysis to determine MI uses the Breeding Risk Index (BRI) and Hygiene Risk Indicator (HRI) indicators. The results of this study indicate that there were 225 containers inside the house observed, where 51 containers of them were found to have Aedes mosquito larvae while outside the house found as many as 28 containers with 8 containers including larvae. The identification results found 2 types of Aedes mosquitoes namely, Ae. aegypti (88.24%) and Ae. albopictus (11.76%). The results of the MI analysis show that Way Halim District is classified as a District with a moderate MI category with an MI value of 46%
Does Expired-Larvicides Impacted to Plankton Abundance and Diversity? Isni Uswatun Khasanah; Endah Setyaningrum; Tugiyono Tugiyono; Gregorius Nugroho Susanto
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 6 No. 2 (2019)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v6i2.45

Abstract

ISOLASI DAN IDENTIFIKASI BAKTERI AEROMONAS HYDROPHILA PADA IKAN LELE DUMBO (Clarias gariepinus) Melly Triana Eryesma Anwar; Tugiyono Tugiyono
Agros Journal of Agriculture Science Vol 25, No 3 (2023): edisi Juli
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jpa.v25i3.3070

Abstract

This research is to isolate, identify and know the pathogenicity of Aeromonas sp . originating from African catfish in Ngawi Regency. Fish that had symptoms of illness and were 20 - 25 cm in size obtained from rearing ponds in 3 sub-districts were used as research samples. Fish kidneys were used for bacterial isolation and inoculated using GSP medium. Furthermore, the characterization and identification of bacteria was carried out by observing the colony morphology, cells, and biochemical properties. Furthermore, Koch's Postulate test was carried out which aims to prove the nature of the pathogen. Furthermore, catfish seeds measuring 7 - 9 cm were measured with a Lethal Dosage of 50 to determine their level of pathogenicity. The results showed that fish were infected with bacteria, where symptoms appeared, namely the skin had ulcers, damage to the kidneys and swelling of the stomach. A total of 15 bacterial isolates obtained consisted of six from Geneng District, five from Karang Jati District and four isolates from Paron District. The identification results showed that Aeromonas hydrophila was identified from 12 isolates, A. salmonicida was identified from three isolates. Isolates A. hydrophila GKJ1, GKJ4, GGN2, GGN5, GPR2 and GPR4 were virulent against these fish.INTISARI Penelitian ini untuk mengisolasi, mengidentifikasi dan mengetahui patogenitias bakteri Aeromonas sp . yang berasal dari lele dumbo di Kabupaten Ngawi. Ikan yang memiliki gejala sakit dan berukuran 20 - 25 cm yang diperoleh dari kolam pembesaran di 3 kecamatan digunakan untuk sampel penelitian. Ginjal ikan digunakan untuk isolasi bakteri dan diinokulasikan menggunakan medium GSP. Selanjutnya dilakukan karakterisasi dan identifikasi bakteri dengan mengamati morfologi koloni, sel, dan sifat biokimia. Selanjutnya dilakukan uji Postulat Koch yang bertujuan untu membuktikan sifat patogen. Selanjutnya benih lele yang berukuran 7 - 9 cm diukur nilai Lethal Dosage 50 untuk mengetahui tingkat patogenitasnya. Hasil menunjukkan ikan terinfeksi bakteri, dimana munculnya gejala yaitu bagian kulit terdapat luka borok, kerusakan pada ginjal dan perut mengalami pembekakan. Total isolasi bakteri yang didapat sebanyak 15 yang terdiri dari enam berasal dari Kecamatan Geneng, lima berasal dari Kecamatan Karang Jati dan empat isolate berasal dari Kecamatan Paron. Hasil identifikasi menunjukkan bahwa bakteri Aeromonas hydrophila teridentifikasi dari 12 isolat, bakteri A. salmonicida teridentifikasi dari tiga isolate. Isolat A. hydrophila GKJ1, GKJ4, GGN2, GGN5, GPR2 dan GPR4 bersifat virulen terhadap ikan tersebut.pathogenicity 
PENGARUH FAKTOR BIOTIK DAN ABIOTIK PADA KEANEKARAGAMAN MAKROBENTOS DI KAWASAN MANGROVE Fatina Azhar; Tugiyono Tugiyono
Agros Journal of Agriculture Science Vol 25, No 3 (2023): edisi Juli
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jpa.v25i3.3305

Abstract

Human activities that often occur in coastal areas affect biota such as mangrove ecosystems and macrobenthos. Benthic biodiversity reflects the slow-growing and sensitive nature of mangrove ecosystems. This study aims to determine the influence of the biotic and abiotic environment on macrobenthos in Cendi Manik Village, Sekotong District, West Lombok Regency, West Nusa Tenggara. Data on macrobenthos, biotic and abiotic environments in natural and restored mangrove forests were collected, and multiple regression was used to analyze macrobenthic species diversity and biotic and abiotic environmental variables. The main plants are Rhizophora mucronata Lam and Avicennia marina Forssk. The native and restored mangroves have a higher macrobenthic biodiversity index (H' 1.207). The macrobenthos similarity between the two places reached 84.6%. The results of the multiple regression test showed that mud thickness and brightness had the greatest effect on macrobenthos. Keywords: beach, macrobenthos, mud thickness, brightness INTISARIAktivitas manusia yang sering terjadi di wilayah pesisir mempengaruhi biota seperti ekosistem mangrove dan makrobenthos. Keanekaragaman hayati bentos mencerminkan sifat ekosistem mangrove yang tumbuh lambat dan sensitif. Penelitian ini bertujuan untuk mengetahui pengaruh lingkungan biotik dan abiotik terhadap makrobentos di Desa Cendi Manik Kecamatan Sekotong Kabupaten Lombok Barat Nusa Tenggara Barat. Data makrobentos, lingkungan biotik dan abiotik di mangrove alami dan restorasi dikumpulkan, dan regresi berganda digunakan untuk menganalisis keanekaragaman spesies makrobentos dan variabel lingkungan biotik dan abiotik. Vegetasi utamanya yaitu Rhizophora mucronata Lam dan Avicennia marina Forssk. Mangrove alami dan yang direstorasi memiliki indeks keanekaragaman hayati makrobentos yang lebih (H’ 1,207). Kemiripan makrobentos antara kedua tempat tersebut mencapai 84,6%. Hasil uji regresi berganda menunjukkan bahwa ketebalan dan kecerahan lumpur memiliki dampak tersebesar pada makrobentos.Kata kunci: pesisir, makrobentos, ketebalan lumpur, kecerahan