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All Journal Majalah Kedokteran Bandung Indonesian Journal of Cancer Jurnal Dinamika Sosial Budaya MAHESA : Malahayati Health Student Journal
Danny Halim
Grup Peneliti Sel Punca, Unit Penelitian Kesehatan, Fakultas Kedokteran Universitas Padjadjaran
Arts Humanities Dentistry Health Professions Medicine & Pharmacology Nursing Public Health

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Cancer Stem Cell: Target Baru Obat Antikanker HALIM, DANNY; DJUWANTONO, TONO; AHMAD, TRI HANGGONO
Indonesian Journal of Cancer Vol 4, No 3 (2010): Jul - Sep 2010
Publisher : "Dharmais" Cancer Center Hospital

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Abstract

Relaps, resistance and metastasis has become prominent problems that oncologists and cancer patients have to dealt with. Various studies have been done previously concluded that there are a subpopulation of cancer cells, identified as cancer stem cell, most likely to be the cause of relaps, resistance and metastasis of cancer. Cancer stem cell is a subpopulation of cancer cells that possess tumorigenicity, hence it can initiate the growth of tumor. Cancer stem cell has been suspected to be originated from normal stem cells reside in mature tissues, or from progenitor cells that gone through some series of alterations on its characteristics, including mutagenic and non-mutagenic changes. As seen in normal stem cells, cancer stem cell is also oftenly found in its inactive state. Therefore, cancer stem cell is not affected when it treated with many chemotherapeutic agents that are targeting cancer cells that proliferate extensively. Eventually, this event leads to the incidence of cancer relaps on cancer patients who already had series of cancer therapy. Based on this knowledge, it can be concluded that the only absolute way to overcome the incidence of metastasis, resistance and relaps on cancer patients, is to targeting cancer stem cell. Therefore, optimization on protocols of cancer stem cell identification and isolation strived continously. Some molecular markers that are oftenly used as a standard on cancer stem cell isolation are CD34, CD44 and CD133. In line with that, isolation methods that are based on sphere formation and the absorption of coloring dye could also be done to obtain cancer stem cell population. This review article would like to explain the nature of cancer stem cell existence, the pathology underlies its formation, characteristics and identification techniques that are commonly used, and challenges that have to be faced by scientists and physicians in order to optimize the application of cancer stem cell theory for the progress of science and patients’ sake.
MODIFIKASI METODE ISOLASI SEL ENDOTEL PEMBULUH DARAH OTAK (EPDO) TIKUS: TEKNIK DASAR KULTUR SEL PRIMER DI BIDANG NEUROSAINS Faried, Ahmad; Zafrullah Arifin, Muhammad; Sutiono, Agung Budi; Halim, Danny; Djuwantono, Tono; Achmad, Tri Hanggono
Majalah Kedokteran Bandung Vol 42, No 4
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Metode konvensional isolasi sel endotel pembuluh darah otak (EPDO) masih tergolong sulit, sehingga upaya mendapatkan populasi murni sel ini adalah tantangan. Pada penelitian ini dilakukan isolasi endotel dari tikus Wistar dan mencit C57/Bl6, berdasarkan protokol the care and use of laboratory animals, Universitas Gunma, Jepang. Modifikasi metode isolasi adalah menggunakan gradasi bovine serum albumin (BSA), bukan Dextran-70 yang umumnya dipakai, untuk memisahkan sel EPDO yang bersatu menjadi sel EPDO tunggal. Penelitian ini dilakukan di laboratorium sel kultur, Universitas Padjadjaran bekerjasama dengan Universitas Gunma, Jepang, Januari 2008?Juni 2009. Uji hasil isolasi dan karakteristik sel EPDO dilakukan dengan teknik imunofloresen. Ekspresi tight junction ZO-1, menunjukkan sel EPDO membentuk selapis sel utuh, rapat, tidak bertumpuk dan kompak, sesuai dengan karakteristik dinding EPDO. Fenotip sel EPDO dikonfirmasi dengan acethylated LDL, faktor von Willebrand dan CD31. Penghancuran kapiler dengan collagenase/dispase masih menghasilkan populasi sel yang terkontaminasi perisit. Kontaminasi dimurnikan dengan menggunakan puromycin, tingkat pemurnian sel EPDO mencapai 98,3%. Simpulan, teknik modifikasi berhasil mengisolasi sel EPDO tikus dan mencit, tanpa melakukan intervensi genetik. Puromycin dapat digunakan untuk memurnikan sel EPDO. [MKB. 2010;42(4):161?8].Kata kunci: Metode modifikasi isolasi sel EPDO, pembuluh sawar otak, teknik pemurnian Isolation Modified-Method of Mouse-Brain Microvessel Endothelial Cells: Primary Cell Culture Technique in NeuroscienceIsolation method to obtain pure BMVECs is hard to be done consistently and remains a challenge. In this study, we isolated BMVECs from Wistar rat and C57/Bl6 mouse from Japan SLC. All procedures performed according to guidelines for the care and use of laboratory animals of Gunma University, Japan. The modification of isolation method was using bovine serum albumin (BSA) gradation, not Dextran-70 in which generally used, to separate clusters of BMVECs into single cell. This study was done at Universitas Padjadjaran, in colaboration with Gunma University, Japan, January 2008?June 2009. Further,characteristic and purification results were proven by imunofluorescene staining. The results showed that staining of tight junction, ZO-1, formed a monolayer, tightly packed, non-overlapping and contact-inhibited BMVECs, as expected for a vessel wall endothelial. ECs phenotype confirmed by acethylated LDL, von Willebrand and CD31. The digestion of capillaries generated contaminating pericytes. Contamination was purified using puromycin and the results considered satisfactory (98.3%). In conclusion, our modification procedure allows the isolation of primary rat and mouse BMVECs, which form an endothelial-like monolayer in few days. Puromycin can be used for purification of primary rat and mouse BMVECs. [MKB. 2010;42(4):161?8].Key words: Blood brain barrier, isolation modified-method of mouse-BMVECs, purification methods DOI: http://dx.doi.org/10.15395/mkb.v42n4.30
Analisis Komunitas Online Girl We Can Terkait Efektivitas Komunikasi Interpersonal antara Pengurus dan Anggota Halim, Danny
Jurnal Dinamika Sosial Budaya Vol. 24 No. 2 (2022): Desember (2022)
Publisher : Universitas Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26623/jdsb.v24i2.3611

Abstract

The Covid-19 pandemic has caused communities that previously worked offline to turn online, and the communities that have been online since the beginning have become more. It also has an impact on the online community of Girl We Can. Changes due to the pandemic require that all activities be carried out virtually, including communication. This study was conducted to determine the effectiveness of interpersonal communication between administrators and members in the Girl We Can. The method used is a qualitative phenomenological approach, and data collection techniques using observation and interviews. The results show that there is still a communication gap between the management and members which has a fatal effect on Girl We Can communication, communication between the management and members is still not effective because of the lack of interaction carried out by the administrators in the Girl We Can WhatsApp group which causes a loss of sense of family and increases sense of individualism. So it s  important for the community to increase the quantity of interaction, hold offline meetings, involve community members in internal management, and always pay attention to communication within the community and the needs of members such as member psychology.
Gambaran Epidemiologi Kasus Tumor Meningioma Intrakranial WHO Derajat II dan III di Rumah Sakit Kanker Dharmais Singh, Arwinder; Firdaus, Muhammad; Widarta, Oskar Ady; Sugiarto, Yosafat Kurniawan; Halim, Danny; Faried, Ahmad
MAHESA : Malahayati Health Student Journal Vol 3, No 11 (2023): Volume 3 Nomor 11 (2023)
Publisher : Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/mahesa.v3i11.11573

Abstract

ABSTRACT Meningioma is the most common primary intracranial tumors in adults. Although most meningioma cases are regarded as benign, certain types have been suggested of having higher proliferative capacities compared to the majority. Knowing the epidemiology of the more aggressive types of meningioma are important to anticipate the disease burden and improve their management.  This study aims to describe the epidemiology of WHO grade II and III intracranial meningiomas at Dharmais National Cancer Center Hospital. All patients diagnosed with WHO grade II or III intracranial meningiomas between 2011 and 2022 were included in this study. Information on patient’s characteristics, tumor location, and histopathological analyses. As many as thirty-three patients diagnosed with WHO grade II and III intracranial meningiomas between 2011 and 2022. Most patients were female (72.72%), aged between 40- to 60-year-old (57.57%), classified as WHO grade II (60.6%), and had their tumors located at convexity regions (48.48%). The most common grade II intracranial meningioma is atypical (95%), while the majority of grade III intracranial meningiomas is anaplastic (76.92%). In both WHO grade II and III intracranial meningiomas, the highest number of patients aged between 40- to 60-year-old, 60% and 53.85%, respectively. Interestingly, most WHO grade II intracranial meningiomas were in skullbase regions (50%); meanwhile, majority of WHO grade III intracranial meningiomas were in convexity regions (69.23%). Although it only represents a minor fraction from the total meningioma cases, patients who are diagnosed with WHO grade II and III intracranial meningiomas are faced with higher risks of morbidity and mortality compared to WHO grade I intracranial meningiomas. The results of this study describe the current epidemiology of this challenging tumor. Keywords : Intracranial, Meningioma, Benign, WHO Grading ABSTRAK Meningioma adalah tumor intrakranial yang paling sering terjadi pada pasien dewasa. Meskipun kebanyakan kasus meningioma intrakranial tergolong jinak, namun beberapa tipe meningioma tertentu terbukti memiliki kapasitas proliferatif yang lebih tinggi dibandingkan dengan mayoritas tipe meningioma. Pengetahuan mengenai epidemiologi dari tipe meningioma yang bersifat agresif sangatlah penting untung mengetahui beban penyakit dan melakukan upaya peningkatan manajemen penyakit ini. Penelitian ini bertujuan untuk mengetahui data epidemiologi meningioma intrakranial derajat 2 dan 3 berdasarkan klasifikasi histopatologis yang ditetapkan oleh badan kesehatan dunia / World Health Organization (WHO). Seluruh pasien yang didiagnosis dengan meningioma intrakranial derajat 2 dan 3 di RS Kanker Dharmais selama periode tahun 2011 s.d. 2022 dimasukkan ke dalam studi ini. Data mengenai karakteristik pasien, lokasi tumor, dan hasil analisis histopatologis diambil dan diolah sebagai data penelitian. Sebanyak 33 pasien didiagnosis dengan meningioma intrakranial derajat 2 dan 3 selama periode tahun 2011 s.d. 2022. Mayoritas pasien adalah perempuan (72.72%), berusia antara 40 s.d. 60 tahun (57.57%), didiagnosis dengan meningioma intrakranial derajat 2 (60.6%), dengan lokasi tumor di daerah konveksitas (48.48%). Jenis meningioma derajat 2 tersering adalah atipikal (95%), sedangkan jenis meningioma derajat 3 tersering adalah anaplastik (76.92%). Rentang usia terbanyak dari pasien penderita meningioma intrakranial derajat 2 (60%) dan 3 (53.85%) adalah 40 s.d. 60 tahun. Dalam hal lokasi tumor, lokasi tumor tersering dari meningioma derajat 2 adalah dasar tengkorak (50%), sedangkan lokasi tumor tersering dari meningioma derajat 3 adalah konveksitas (69.23%). Meskipun mayoritas pasien yang didiagnosis dengan meningioma intrakranial tergolong sebagai derajat 1, namun pasien meningioma intrakranial derajat 2 dan 3 memiliki resiko morbiditas dan mortalitas yang lebih tinggi. Hasil dari penelitian ini memaparkan data epidemiologi terkini dari jenis meningioma intrakranial yang terbilang lebih agresif. Kata Kunci: Meningioma, Intrakranial, Jinak, Derajat WHO
Co-Authors Agung Budi Sutiono Ahmad Faried Muhammad Firdaus Muhammad Zafrullah Arifin Singh, Arwinder Sugiarto, Yosafat Kurniawan Tono Djuwantono Tri Hanggono Achmad TRI HANGGONO AHMAD, TRI HANGGONO Widarta, Oskar Ady