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All Journal MARINA CHIMICA ACTA Indonesia Chimica Acta Nusantara Medical Science Journal Indonesian Journal of Chemistry

Ahyar Ahmad

Chemistry Department, Faculty Of Mathematics And Natural Sciences, Hasanuddin University

Author-ID : 2734467

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Materials Science & Nanotechnology Neuroscience

Published : 11 Documents Claim Missing Document

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PROFILE OF AMINO ACIDS OF FOREST HONEYS FROM MALLAWA SOUTH SULAWESI Mahdalia Mahmud; Alfian Noor; Ahyar Ahmad
Marina Chimica Acta Vol. 19 No. 1 (2018): Volume 19, No. 1 (2018)
Publisher : Hasanuddin University

Mallawa is one of the areas that has production forests as the second largest honey producer in Maros. The aim of this research determined the profile of amino acids in honey. Amino acids of two forest honeys were analyzed using ultra performance liquid chromatography (UPLC). The main amino acids detected honeys were glutamic acid (1060.66 mg/kg, 928.85 mg/kg), histidine (700.37 mg/kg, 478.02 mg/kg), and arginin (683.11 mg/kg, 665.08 mg/kg), but tyrosine and serine not detected. Some other amino acids, including threonine, leucine, valine, phenylalanine and proline, displayed similar values contents of 239.36 mg/kg, 268.15 mg/kg; 225.81 mg/kg, 293.44 mg/kg; 170.12 mg/kg, 226.03 mg/kg; 174.74 mg/kg, 288.11 mg/kg; and 161.58 mg/kg, 257.44 mg/kg, respectively.

SURFACE TEMPERATURE DISTRIBUTION ANALYSIS USING REMOTE SENSING SYSTEM IN SPERMONDE ESTUARY Rakhmad Armus; Alfian Noor; Ahyar Ahmad; Muhammad Lukman
Marina Chimica Acta Vol. 19 No. 2 (2018): Volume 19, No. 2 (2018)
Publisher : Hasanuddin University

Research on the phenomenon of climate change on the region Makassar Strait waters that are part of the oceanographic activities that require observation of the sea surface temperature (SST), surface currents and ocean color imagery from satellite imagery. In this case, satellite remote sensing data using Aqua-Modis analyze both visually and raw-surface temperature parameter data in order to study a variety of other related phenomena that take place in the ocean. The existence of satellite imagery data for the observation of parameters and / or oceanographic phenomena will be more profitable in terms of time and cost and high accuracy. The purpose of this study was to review based on the description of the SST analysis using Aqua-Modis image data (non-commercial) and open source software in providing the information cepatdan applicable. Bodies Spermonde been considering the seas is quite unique because it is located right in the path of the current meeting of the Pacific Ocean through the sea of Sulawesi and spacious enough for the continuity of mesoscale oceanographic processes for coverage in the waters Spermonde in (islands) Indonesia

PLANKTON ABUNDANCE BETWEEN DRY AND RAINY SEASON IN TALLO FRESHWATER AND PANGKEP RIVER Rakhmad Armus; Alfian Noor; Ahyar Ahmad; Muhammad Lukman
Marina Chimica Acta Vol. 20 No. 2 (2019): Volume 20, No. 2 (2019)
Publisher : Hasanuddin University

Plankton abundance is influenced by seasons, sea surface currents (SSC), nutrients and surface temperature (SST). Is a marine organism has a function to stabilize the surface temperature of the earth by regulating the concentration of Dimetyl sulfide (DMS) due to exposure to direct sunlight. There is the ability of the ocean to regulate warming and to regulate the distribution of water vapor in the atmosphere which is controlled by sea surface temperature. Sampling using the transeck system in September and January represents the rainy season and the dry season which is closest to the Tallo river estuary and the Pangkep river estuary represents the estuary region, towards the coast of the spermonde (Litoral) islands and far from the coast (offshore) from freshwater sources, but the relationship of nutrient entry into estuary areas by sea surface currents (SSC) is still poorly known. The dominant aspect of phytoplankton is determined by the amount of nutrients flowing (runoff) from land to estuary to spermonde waters

Isolasi, Karakterisasi, dan Uji Bioaktivitas Metabolit Sekunder dari Hydroid Aglaophenia cupressina Lamoureoux Sebagai Bahan Dasar Antimikroba Eva Johannes; Hanapi Usman; Ahyar Ahmad
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 2 No 1 - June 2009
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v2i1.972

Isolation, structure elucidation, activity test against Salmonella thypi, Staphylococcus aureus of secondary metabolite from hydroid Aglaophenia cupressina Lamoureoux, were conducted. This study was aimed to identify and isolate the compound with antibacterial properties of hydroid Aglaophenia crupessina Lamoureoux. This study used stepwise method with the following sequences: extraction, isolation, purification, structure elucidation, and bioactivity test. Study results indicated three compounds found: (1)carboxylate acid compound: hexadecanoic acid and has antibacterial properties; (2) alkaloidcompound which was considered as a new compound: aglaounhas and has antimicrobialproperties; (3) steroid compound: β-sitosterol with no antimicrobial properties.Keywords: Isolation, characterization, secondary metabolite, Aglaophenia cupressina, Antibacterial.

Pengaruh Konsentrasi Glutaraldehid Pada Komposisi Membran Elektroda Biosensor Pestisida Karbamat Mashuni Mashuni; Muhammad Syahrul; Ahyar Ahmad; Abdul Wahid Wahab
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 3 No 1 - June 2010
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v3i1.5970

In recent years carbamic pesticides, which show low environmental persistence but a high acute toxicity. Their presence in environmental and food poses a potential hazard to human health and there is a growing interest in their rapid and accurate determination for food safety and environmental monitoring. The aim of this research is to design biosensor for analyzing carbamate pesticides residue in sample show with composite variable concentrate glutaraldehyde (GA) in electrode membrane. Enzim acetylcholinesterase (AChE) was co-immobilised with choline oxidase (ChO) onto a platinum (Pt) surface using a solution of 10% cellulose acetate and 15%, 20%, 25% glutaraldehyde. Result of this research show that glutaraldehyde 15% the detection limit is 10-7,8 M for glutaraldehyde 20% the detection limit is 10-8,4 M and glutaraldehyde 25% the detection limit is 10-8,7 M. This a results is approximately equal to 0,0022 ppm or 2,2 ppb – 0,0002 ppm or 0,2 ppb, which means that this biosensor is sensitive for determining carbamates pesticides residue where its detection limit is comparable to to the detection limit of conventional instrument such as Gas Chromatography (GC) and High Pressure Liquid Chromatography (HPLC), i.e. respectively 1,5 ppb and 2,0 ppb. The proposed electrochemical pesticide sensitivity test exhibited high sensitivity, desirable accuracy, low cost and simplified procedure.Keywords: Biosensors, glutaraldehyde, cellulose acetate, immobilised, carbamate Biosensors, glutaraldehyde, cellulose acetate, immobilised, carbamate pesticides

EFFECT OF METAL ION Cu (II) and Mg (II) ON THE ACTIVITIES ANTIOXIDANT ANTHOCYANIN OF EXTRACT ETHANOL SKIN DRAGON FRUIT RED(Hylocereuspolyrhizus) Asrul Sani; Ahyar Ahmad; Firdaus Zenta
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 11, No.1: June 2018
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v11i1.6400

The effect of Cu2+ and Mg2+ metal ions on the antioxidant activity of anthocyanins of ethanol extract from the red dragon skin fruit (Hylocereus polyrhizus) has been done. Anthocyanin pigments from ethanol extract the red dragon skin fruit, content and antioxidant activity of anthocyanins from the red dragon fruit skin with ethanol solvent, and the effect of Cu2+ and Mg2+ metal ions on the antioxidant activity of anthocyanins. Identification of anthocyanin was done by identification of color test, using spectrophotometer analysis of UV-Vis and FTIR. The anthocyanin content was determined using pH difference method, and determined effect of metal ions extract was done by adding 50 ppm, 100 ppm, and 150 ppm of metal ions Cu2+ and Mg2+ into the anthocyanin extract that was been acidified by HCl , using spectrophotometer analysis of FTIR. Antioxidant activity test using DPPH method. The results show that ethanol extracts the red dragon anthocyanin by content is 12.5241 mg / L and antioxidant activity (IC50) is 0.478 μg / mL and with addition of2+ and Mg2+ metal ions can be increased antioxidant activity (IC50) of anthocyanins to be 0.2259 μg / mL for Cu2+ at concentration 50 ppm and 0.3351 μg / mL for Mg2+ at concentration 50 ppm.

EFFECT OF Mg (II) METAL ION ON ANTIOXIDANT ACTIVITIES OF ETHANOL EXTRACTS OF RAMBUTAN PEEL (Nephelium lappaceum) Emmi Astuti; Ahyar Ahmad; Seniwati dali
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 11, No.1: June 2018
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v11i1.6403

Anthocyanin is the one of natural dyes that often used in foodstuffe, because anthocyanin can make a more color like red, blue and purple. Anthocyanin storage can make anthocyanin is damage, because the interaction with tempherature, light, and oxygen show that can given effect about the stability anthocyanin color, and decrease the anthocyanin as an antioxidant. That problem can be solved with copigmentation, that is an anthocyanin reaction with metal ion. In this study can showed that ethanol extract from Rambutan peel and we obtained 23.824 gram with total anthocyanin content of 709.3866 mg/L with antioxidant activity of 0.0046 μg/mL, and then the extract of anthocyanin with metal ion Mg2+ in concentration 50; 100 and 150 ppm had IC50 are 0.0061; 0.0048 and 0.0067 μg/mL. This conclution from the added of metal ion Mg2+ showed that has not show much different antioxidant activity.

DETECTION OF SHIGELLA SP ON STOOL CHILDREN WITH DIARRHEA USING CULTURE METHODE AND PCR Rusdianto Rusdianto; Muh Nasrum Massi; Ahyar Ahmad
Nusantara Medical Science Journal Volume 1 No. 4 Oktober - Desember 2016
Publisher : Faculty of Medicine, Hasanuddin University.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/nmsj.v1i4.2250

ABSTRACT Introduction: Diarrhea is one health problem in the world with mortality and morbidity rates are high, both in developed and developing countries. This research aims to detect Shigella sp in the stool of children with diarrhea using culture method and PCR. Methods: This research is a descriptive with cross-sectional design. A total of 50 samples were obtained from four health centers in Makassar city, i.e Barabaraya, Pampang, Antang Perumnas and Tamangapa from May - July 2016 with rectal swab method. For culture test, the samples were inoculated on medium SSA. While with PCR test, primer icsA (526 bp) was used. Results: The results indicate that, from 50 samples tested by using culture method, there is no sample (0%) posi- tive detected Shigella sp. While the PCR method there 6 samples (24%) positive detected Shigella sp. Conclusion: The sensitivity of PCR in the detection of Shigella sp from the level of dilution 100 - 10-3. Al together, it can be inferred that PCR method is more accurate method detect Shigellasp than other culture methode. Keywords: Diarrhea, Shigellasp, Culture, PCR

DETECTION OF ESCHERICHIA COLI IN CHILDREN STOOL WITH DIARRHEA PATIENTS USING CULTURE AND POLYMERASE CHAIN REACTION METHOD Lokita Purnamasari; Muh Nasrum Massi; Ahyar Ahmad
Nusantara Medical Science Journal Volume 2 No. 1 Januari - Juni 2017
Publisher : Faculty of Medicine, Hasanuddin University.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/nmsj.v2i1.2256

ABSTRACT Introduction : Diarrheal diseases are a serious issue on health in developing countries and the liquid cause’s morbidity and mortality in children that cause the diarrhea pathogenic bacte- ria including Escherichia coli. This research aims to detect E. coli in stool diarrhea patient of the child with culture method and Polymerase Chain Reaction. Methods : This research is descriptive research design with cross-sectional approach. The sampling was collected from some public health centers namely Pampang, Barabarayya, Antang Perumnas, Tamangapa public health centers. Specimen testing process was conducted at Microbiology laboratory of the Teaching Hospital University of Hasanuddin to identify E. coli bacterium with the culture and to detect eae gene and bfp with PCR technique. Results : The results indicate that from50 samples, there are 15 samples (30%) of samples positively detection E. coli using culture method and PCR test using eae and bfp primer found 20 (40%) and 1 sample (2%) positive enteropathogen E. coli. Conclusion : PCR methods indicated the result of E. coli bacteria results faster and more accurate that other culture methods. Keywords : Diarrhea, Escherichia coli, Polymerase Chain Reaction

Purification and Characterization of Amyloglucosidase Enzyme from the Thermophilic Endomycopsis fibuligera Using Sago Starch as a Substrate Ahyar Ahmad; Harningsih Karim
Indonesian Journal of Chemistry Vol 16, No 3 (2016)
Publisher : Universitas Gadjah Mada

An investigation on purification and characterization of amyloglucosidase enzyme from Endomycopsis fibuligera by fermentation of sago starch has been carried out. This enzyme is inductive and can be produced by fermenting sago starch in a medium containing E. fibuligera. Crude enzyme was obtained by centrifuging the medium cultures containing E. fibuligera at 6,000 rpm for 20 min and then adding with 0.15 M acetate buffer (pH 5.0). Enzyme activity was determined using Somogyi-Nelson method by quantifying the released glucose from amyloglucosidase catalysis of starch (0.2%) as substrate. Prepurification process was conducted by ammonium sulphate fractionation and it showed that the ammonium sulphate fractionation with the degree of saturation of 40-60% produced a maximum activity of enzyme. Purification by DEAE-Cellulose and Sephadex G-75 column chromatography produced three and one fractions with purifity 17.4 and 22.5 times, respectively, compared to the crude extract enzyme. Characterization of this enzyme showed the optimum condition at pH 5.0 and 55 °C with 0.2% starch as substrate. The amyloglucosidase activities was strongly increased by addition of Co2+ and Mn2+ ions, whereas the activities were weakly decreased by addition of K+, Mg2+, and Fe3+ ions.

Co-Authors Abdul Wahid Wahab Alfian Noor Alfian Noor Alfian Noor Armus, Rakhmad Asrul Sani Emmi Astuti Eva Johannes Firdaus Zenta Hanapi Usman Harningsih Karim Lokita Purnamasari Mahdalia Mahmud Mashuni Mashuni Muh. Nasrum Massi Muhammad Lukman Muhammad Syahrul Rusdianto Rusdianto Seniwati Dali

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