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All Journal Indonesian Journal of Chemistry Fullerene Journal of Chemistry Medika Kartika : Jurnal Kedokteran dan Kesehatan JURNAL PENGABDIAN MASYARAKAT YAMASI JURNAL KESEHATAN YAMASI MAKASSAR
Harningsih Karim
Departemen Farmasi,Akademi Farmasi YAMASI, Kota Makassar, Sulawesi Selatan, Indonesia
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Purification and Characterization of Amyloglucosidase Enzyme from the Thermophilic Endomycopsis fibuligera Using Sago Starch as a Substrate Ahyar Ahmad; Harningsih Karim
Indonesian Journal of Chemistry Vol 16, No 3 (2016)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (429.249 KB) | DOI: 10.22146/ijc.21147

Abstract

An investigation on purification and characterization of amyloglucosidase enzyme from Endomycopsis fibuligera by fermentation of sago starch has been carried out. This enzyme is inductive and can be produced by fermenting sago starch in a medium containing E. fibuligera. Crude enzyme was obtained by centrifuging the medium cultures containing E. fibuligera at 6,000 rpm for 20 min and then adding with 0.15 M acetate buffer (pH 5.0). Enzyme activity was determined using Somogyi-Nelson method by quantifying the released glucose from amyloglucosidase catalysis of starch (0.2%) as substrate. Prepurification process was conducted by ammonium sulphate fractionation and it showed that the ammonium sulphate fractionation with the degree of saturation of 40-60% produced a maximum activity of enzyme. Purification by DEAE-Cellulose and Sephadex G-75 column chromatography produced three and one fractions with purifity 17.4 and 22.5 times, respectively, compared to the crude extract enzyme. Characterization of this enzyme showed the optimum condition at pH 5.0 and 55 °C with 0.2% starch as substrate. The amyloglucosidase activities was strongly increased by addition of Co2+ and Mn2+ ions, whereas the activities were weakly decreased by addition of K+, Mg2+, and Fe3+ ions.
EXPRESSION AND FUNCTIONAL INTERACTION OF CHICKEN RbAp46 POLYPEPTIDE WITH HISTONES, HISTONE DEACETYLASE-1, AND HISTONE ACETYLTRANSFERASE-1 Ahyar Ahmad; Harningsih Karim
Indonesian Journal of Chemistry Vol 13, No 2 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (410.548 KB) | DOI: 10.22146/ijc.21296

Abstract

In this study, we cloned and sequenced cDNA encoding the chicken p46 polypeptide, RbAp46. The cDNA encoding a protein consists of 424 amino acids is a member of the WD protein family, with seven WD repeat motifs, and exhibits 90.3% identity to RbAp48, and 94.3% identity to the human RbAp46. The RbAp46 fusion protein were synthesized by in vitro translation system and in Escherichia coli under induction by 50 µM IPTG and single step purified with glutathione-Agarose beads, showed that GST-tagged protein of approximately 72 kDa. The in vitro experiment established that RbAp46 interacts with chicken histones, chHDAC-1, and chHAT-1. The in vitro immunoprecipitation experiment, involving truncated mutants of RbAp46, revealed not only that two regions comprising amino acids 33-179 and 375-404 are necessary for its binding to H2B, but also that two regions comprising amino acids 1-32 and 405-424 are necessary for its binding to H4. Furthermore, the GST pulldown affinity assay, involving truncated mutants of RbAp46, revealed that a region comprising amino acids 359-404 binds to chHAT-1 in vitro. Taken together, these results indicate not only that RbAp46 should participate differentially in a number of DNA-utilizing processes through interactions of its distinct regions with histones and chHAT-1, but also that the proper propeller structure of RbAp46 is not necessary for its interaction with chHAT-1.
MUTATION ON WD DIPEPTIDE MOTIFS OF THE p48 SUBUNIT OF CHROMATIN ASSEMBLY FACTOR-1 CAUSING VIABILITY AND GROWTH OF DT40 CHICKEN B CELL LINE Ahyar Ahmad; Harningsih Karim
Indonesian Journal of Chemistry Vol 10, No 2 (2010)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (795.816 KB) | DOI: 10.22146/ijc.21468

Abstract

Chromatin assembly factor-1 (CAF-1), a protein complex consisting of three subunits, p150, p60, and p48, is highly conserved from yeast to humans and facilitated nucleosome assembly of newly replicated DNA. The p48 subunit, CAF-1p48 (p48), with seven WD (Trp-Asp) repeat motifs, is a member of the WD protein family. The immunoprecipitation experiment revealed that ß-propeller structure of p48 was less stringent for it's binding to HDAC-1, but more stringent for its binding to both histones H4 and CAF-1p60 but not to ASF-1, indicating that the proper ß-propeller structure of p48 is essential for the binding to these two proteins histone H4 and CAF-1p60. Complementation experiments, involving missense and truncated mutants of FLAG-tagged p48, revealed that mutations of every of seven WD dipeptide motifs, like both the N-terminal and C-terminal truncated mutations, could not rescue for the tet-induced lethality. These results indicate not only that p48 is essential for the viability of vertebrate cells, although the yeast p48 homolog is nonessential, but also that all the seven WD dipeptide motifs are necessary for the maintenance of the proper structure of p48 that is fundamentally important for cell viability.
Review on Anticancer Activity of Essential Metal Dithiocarbamate Complexes Rizal Irfandi; Indah Raya; Ahyar Ahmad; Ahmad Fudholi; Hasnah Natsir; Desy Kartina; Harningsih Karim; Santi Santi; Subakir Salnus
Indonesian Journal of Chemistry Vol 22, No 6 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.73738

Abstract

The importance of essential metal ions and their metal complexes in the creation of prospective medical therapies has long been recognized. In chemistry, molecular biology, and medicinal fields; the interaction of metal complexes with DNA has been a subject of study. The dithiocarbamate essential metal complex is described extensively in the literature for its various benefits and advantages. With proper use of ligands, it is proven to increase the cytotoxic activity of metal complexes against cancer cells. Some researches have shown significant progress regarding the biological activities of the dithiocarbamate essential metal complex as antimicrobial, antioxidant, and anticancer agents. Metal complexes form complexes with dithiocarbamate ligands with unique structural variations. In this study, we presented an overview of the cytotoxic effects of some dithiocarbamate essential metal complexes on cancer cells, as well as fresh approaches to the design of essential metal-based therapeutics containing dithiocarbamate and molecular targets in cancer therapy. This review may provide an update on recent developments in the medicinal use of essential metals with dithiocarbamate ligands, carried out to identify recent relevant literature. Finally, we predict that the essential metal complexed with dithiocarbamate can be a new breakthrough in the future development of cancer drugs.
MORFOLOGI DERMATOFITA PADA KUKU DAN KULIT SELA JARI KAKI PENDERITA DIABETES MELITUS DI KECAMATAN MAMAJANG KOTA MAKASSAR Anita Anita; Ahyar Ahmad; Hasnah Natsir; Muh.Rifo Rianto; Hasnah Sarean; Harningsih Karim; Nur Hidayah Bano
Medika Kartika : Jurnal Kedokteran dan Kesehatan Vol 5 No 4 (2022): Medika Kartika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran Universitas Jenderal Achmad Yani

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Abstract

Diabetes Melitus merupakan kelompok penyakit metabolik dengan karakteristik hiperglikemia akibat kelainan sekresi insulin. Tingginya kadar glukosa pada kulit dapat berkontribusi terhadap manifestasi kulit sehingga rentan terinfeksi jamur, di antaranya jamur dermatofita. Dermatofita ini merupakan golongan jamur yang melekat dan tumbuh pada jaringan keratin seperti kulit dan kuku, sebagai sumber nutrisi untuk membentuk kolonisasi. Tujuan penelitian ini untuk mengidentifikasi jamur dermatofita serta mengetahui morfologi dermatofita pada kuku dan kulit sela jari kaki penderita diabetes melitus di Kecamatan Mamajang, Kota Makassar. Subjek pada penelitian ini adalah 15 orang penderita diabetes melitus, dengan sampel berupa kerok kuku dan kulit sela jari kaki. Hasil penelitian yang dilakukan pemeriksaan makroskopik dan mikroskopik, ditemukan dua sampel yang jamur dermatofita yaitu Trichophyton rubrum dan Microsporum gypseum, sedangkan sampel lainnya ditemukan jamur non dermatofita, antara lain Candida albicans, Mucor michelli, Aspergillus fumigatus, Aspergillus flavus, dan Aspergillus niger. Kesimpulan penelitian ini adalah ditemukan dua jamur dermatofita spesies Trichophyton rubrum dan Microsporum gypseum pada kuku dan kulit sela jari kaki penderita diabetes melitus di Kecamatan Mamajang, Kota Makassar. Kata kunci : dermatofita, diabetes melitus, microsporum gypseum, trichophyton rubrum DOI : 10.35990/mk.v5n4.p388-400
PENYULUHAN DAN PELATIHAN PEMBUATAN PEWANGI PAKAIAN PADA IBU PKK DESA JONJO KECAMATAN PARIGI KABUPATEN GOWA Sukirawati; Yusriyani; Agust Dwi Djajanti; Harningsih Karim; Ermawati; Maulana Zulkarnain Imansyah; Nirwana Ibrahim; Dhandy Dharmawan; Fauzan Nursyawal
JURNAL PENGABDIAN MASYARAKAT YAMASI Vol. 1 No. 1 (2022): Jurnal Pengabdian Masyarakat Yamasi
Publisher : Akademi Farmasi Yamasi Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (551.028 KB) | DOI: 10.59060/jpmy.v1i1.183

Abstract

Perfume is very familiar with everyday life to increase selfconfidence in socializing. The training for making laundry perfume in Jonjo village is aimed at making people able to make their own perfumes so that they can grow confident and can also be used as business fields. The dedication method that we do is by explaining the perfume formula followed by the practice of making pefume directly involving the service participants. Furthermore, after the laundry perfume is finished, it is packaged and then distributed to the service participants. This activity was attended by 6 AKFAR Yamasi lecturers and 3 AKFAR Yamasi Makassar students, Pak Desa Jonjo along with 5 staff and 20 PKK women in Jonjo village was very enthusiastic in participating in the training for making laundry perfume.
PEMANFAATAN BAHAN ALAM SEBAGAI ALTERNATIF KOSMETIK ALAMI PADA REMAJA: Utilization Of Natural Ingredients As An Alternative To Natural Cosmetics In Adolescents Arief Azis; Harningsih Karim; Ermawati; Yuyun Sri Wahyuni; Muhammad Tahir; Maulana Zulkarnain Imansyah
JURNAL PENGABDIAN MASYARAKAT YAMASI Vol. 1 No. 1 (2022): Jurnal Pengabdian Masyarakat Yamasi
Publisher : Akademi Farmasi Yamasi Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (464.182 KB) | DOI: 10.59060/jpmy.v1i1.186

Abstract

Telah dilakukan kegiatan pengabdian kepada masyarakat dengan tujuan untuk mengenalkan pemanfaaatan bahan alam sebagai alternatif kosmetik kepada remaja.Tampil cantik adalah dambaan remaja yang sedang tumbuh menuju dewasa. Pada masa ini, remaja lebih senang berdandan, karena itu, siswi remaja banyak yang menggunakan produk skin care dan kosmetik ke sekolah. Seiring perkembangan zaman, kosmetik merupakan kebutuhan sehari-hari. Melihat banyaknya para wanita gemar menggunakan make up, banyak produk kecantikan yang ditawarkan baik dari segi harga, tekstur, formula hingga jenis. Namun ada beberapa produk kosmetik yang aman untuk kulit remaja dan tidak baik jika tercampur dengan bahan kimia. Banyak kosmetik yang tidak layak beredar di pasaran saat ini karena mengandung bahan yang tidak diizinkan digunakan di dalam kosmetik dan tidak mempunyai nomor registrasi. Bahan tersebut antara lain merkuri, hidrokinon, dan asam retinoat. Kesadaran akan kandungan kimia yang berbahaya bagi wajah menjadi pertimbangan kuat banyak wanita yang beralih menggunakan kosmetik berbahan herbal. Dengan penggunaan bahan herbal, wajah cantik tanpa efek samping jangka panjang. Kosmetik alami adalah produk kecantikan tanpa bahan kimia berbahaya yang mudah dibuat. Ada beberapa bahan herbal dengan khasiatnya masing-masing dan banyak dijadikan untuk kosmetik, antara lain: Kumyit, Lidah Buaya, Tomat., Seledri. Kegiatan ini dilaksanakan secara daring melalui aplikasi zoom, dengan jumlah peserta 10 orang dari SMK Farmasi Manado. Pemahaman peserta dilakukan melalui diskusi pemahaman materi. Kata kunci :Bahan alam, Kosmetik Remaja.
UJI DAYA HAMBAT KRIM EKSTRAK KULIT BUAH NANAS (Ananas comosus L.) TERHADAP Pseudomonas aeroginosa harningsih karim
Jurnal Kesehatan Yamasi Makassar Vol. 3 No. 1 (2019): Jurnal Kesehatan
Publisher : Akademi Farmasi Yamasi Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (250.477 KB)

Abstract

Nanas (Ananas comosus L.) merupakan salah satu diantara tumbuh- tumbuhan berkhasiat obat yangmengandung vitamin A dan C, kalsium, fosfor, magnesium, natrium, kalium dan enzim bromelin. Bagian buahnanas yang bersifat buangan seperti kulit buah yang memiliki tekstur yang tidak rata dan berduri kecil padapermukaan luarnya juga mengandung zat berkhasiat. Kulit buah nanas mengandung flavonoid, alkaloida,saponin dan steroid. Penelitian ini bertujuan untuk mengetahui apakah krim ekstrak kulit buah nanas dapatmenghambat pertumbuhan bakteri Pseudomonas aeruginosa dengan menggunakan konsentrasi ekstrak kulitbuah nanas 2% dan 5%.Hasil penelitian ini menunjukkan bahwa krim ekstrak kulit buah nanas dapat menghambat pertumbuhanbakteri Pseudomonas aeruginosa dengan rata-rata pada konsentrasi 2% sebesar daerah zona hambat 6,63 mmdan pada konsentrasi 5% sebesar 13,6 mm.Kata Kunci :Nanas, Pseudomonas aeruginosa, Ananas comosus L.
Sintesis Selulosa Suksinat Melalui Reaksi Esterifikasi Asam Suksinat Miftahul Jannah; Ahyar Ahmad; Paulina Taba; Harningsih Karim
Jurnal Kimia Fullerene Vol 8 No 1 (2023): Fullerene Journal of Chemistry
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37033/fjc.v8i1.499

Abstract

Cellulose is a biopolymer that is widely applied in various fields. Modification of cellulose is needed to increase the added value of cellulose. One of these cellulose derivatives is cellulose succinate. This study aims to synthesize succinic cellulose using succinic acid. This research covers the synthesis of cellulose succinate via esterification reaction with a variation of succinic acid (1.6%; 2.4%; 3.2% and 4.0%), DS determination by titration method, and characterization with FTIR. Based on the synthesis results, the optimum DS was obtained at 2.4% succinic acid (SS3) concentration, namely 0.692. The results of the characterization with FTIR also showed the presence of a C=O absorption band indicating the presence of cellulose succinate monoester.
The Effect of Thermal Treatment on the Characteristics of Porous Ceramic-Based Natural Clay and Chitosan Biopolymer Precursors Suriati Eka Putri; Ahyar Ahmad; Indah Raya; Rachmat Triandi Tjahjanto; Rizal Irfandi; Harningsih Karim; Susilo Sudarman Desa; Abd Rahman
Indonesian Journal of Chemistry Vol 23, No 3 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.80375

Abstract

This study was conducted to determine the role of thermal treatment on the crystallinity and pore characteristics of porous ceramic, which was prepared from natural clay (NC) and chitosan (CS) biopolymer using the gel casting method. CS was used as an environmentally friendly pore-forming agent. The applied temperature treatment was based on thermal analysis (TGA/DTA) results and followed a sintering temperature of 900 to 1100 °C. The results showed that at sintering temperatures from 900 to 1000 °C, the crystallinities of the ceramic decrease (from 76.06 to 74.06%) and the crystallite size decreases (from 35.71 to 34.47 nm) while the lattice strain increases (calculated from the Full Width at Half Maximum (β) of the diffraction peak). The highest porosity of ceramic occurred at a sintering temperature of 1000 °C of 37.82 ± 0.19, but the formation of heterogeneous microstructure was observed. The resulting pore size for all temperature treatments was almost mesoporous (19.1 Å). Based on the results obtained, it is emphasized that the sintering temperature can be used to adjust the porosity and microstructure of porous ceramics.
Co-Authors A. Tenriugi Daeng Pine Abd Rahman Agust Dwi Djajanti Agust Dwi Djajanti Ahmad Fudholi Ahyar Ahmad Ahyar Ahmad Ahyar Ahmad Ahyar Ahmad Ahyar Ahmad Ahyar Ahmad Ahyar Ahmad Anita Anita Base, Nurul Hidayah Desy Kartina Dhandy Dharmawan Dwi Sasongko Mardiono Ermawati Ermawati Fauzan Nursyawal Hasnah Natsir Hasnah Natsir Hasnah Sarean Hernawati Basir Indah Raya Indah Raya Irfandi, Rizal Irman Idrus Junaidin Maulana Zulkarnain Imansyah Maulana Zulkarnain Imansyah Maulana Miftahul Jannah Mora Muh. Rifo Rianto Muhammad Nur Alam Muhammad Tahir Nirwana Ibrahim Nur Hidayah Bano Paulina Taba Paulina Taba Rachmat Triandi Tjahjanto Raymond Arief N Noena Santi Santi Sitti Fauziah Noer Subakir Salnus Sukirawati Sukirawati Suriati Eka Putri Suriati Eka Putri Susilo Sudarman Desa Tahirah Hasan Yasnidar Yusriyani Yusriyani Yuyun Sri Wahyuni Zakiah Thahir