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All Journal MARINA CHIMICA ACTA Indonesia Chimica Acta Nusantara Medical Science Journal Indonesian Journal of Chemistry
Ahyar Ahmad
Chemistry Department, Faculty Of Mathematics And Natural Sciences, Hasanuddin University
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Materials Science & Nanotechnology Neuroscience

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Journal : Nusantara Medical Science Journal

 1 
DETECTION OF SHIGELLA SP ON STOOL CHILDREN WITH DIARRHEA USING CULTURE METHODE AND PCR Rusdianto Rusdianto; Muh Nasrum Massi; Ahyar Ahmad
Nusantara Medical Science Journal Volume 1 No. 4 Oktober - Desember 2016
Publisher : Faculty of Medicine, Hasanuddin University.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/nmsj.v1i4.2250

Abstract

ABSTRACT Introduction: Diarrhea is one health problem in the world with mortality and morbidity rates are high, both in developed and developing countries. This research aims to detect Shigella sp in the stool of children with diarrhea using culture method and PCR. Methods: This research is a descriptive with cross-sectional design. A total of 50 samples were obtained from four health centers in Makassar city, i.e Barabaraya, Pampang, Antang Perumnas and Tamangapa from May - July 2016 with rectal swab method. For culture test, the samples were inoculated on medium SSA. While with PCR test, primer icsA (526 bp) was used. Results: The results indicate that, from 50 samples tested by using culture method, there is no sample (0%) posi- tive detected Shigella sp. While the PCR method there 6 samples (24%) positive detected Shigella sp. Conclusion: The sensitivity of PCR in the detection of Shigella sp from the level of dilution 100 - 10-3. Al together, it can be inferred that PCR method is more accurate method detect Shigellasp than other culture methode. Keywords: Diarrhea, Shigellasp, Culture, PCR
DETECTION OF ESCHERICHIA COLI IN CHILDREN STOOL WITH DIARRHEA PATIENTS USING CULTURE AND POLYMERASE CHAIN REACTION METHOD Lokita Purnamasari; Muh Nasrum Massi; Ahyar Ahmad
Nusantara Medical Science Journal Volume 2 No. 1 Januari - Juni 2017
Publisher : Faculty of Medicine, Hasanuddin University.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/nmsj.v2i1.2256

Abstract

ABSTRACT Introduction : Diarrheal diseases are  a serious issue on health in developing countries and the liquid cause’s morbidity and mortality in children that cause the diarrhea pathogenic bacte- ria including Escherichia coli.  This research aims to detect E. coli in stool diarrhea patient of the  child with culture method and  Polymerase Chain Reaction. Methods : This research is descriptive research design with cross-sectional approach. The sampling was collected from some public health centers namely Pampang, Barabarayya, Antang  Perumnas, Tamangapa public health centers. Specimen testing process was conducted at Microbiology laboratory of the Teaching Hospital University of Hasanuddin to identify E. coli bacterium with the culture and  to detect eae gene and bfp with PCR technique. Results : The results indicate that from50 samples, there  are  15 samples (30%) of samples positively detection E. coli using culture method and  PCR  test using eae and  bfp primer found 20 (40%) and  1 sample (2%) positive enteropathogen E. coli. Conclusion : PCR  methods indicated the  result of E. coli bacteria results faster and more accurate that other  culture methods. Keywords : Diarrhea, Escherichia coli, Polymerase Chain Reaction
Co-Authors Abdul Wahid Wahab Alfian Noor Alfian Noor Alfian Noor Armus, Rakhmad Asrul Sani Emmi Astuti Eva Johannes Firdaus Zenta Hanapi Usman Harningsih Karim Lokita Purnamasari Mahdalia Mahmud Mashuni Mashuni Muh. Nasrum Massi Muhammad Lukman Muhammad Syahrul Rusdianto Rusdianto Seniwati Dali